NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results

Abstract Epilepsy in children is the most frequent, heterogeneous and difficult to classify chronic neurologic condition with the etiology found in 35–40% of patients. Our aim is to detect the metabolic differences between the epileptic children and the children with no neurological abnormalities in...

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Autores principales:	Łukasz Boguszewicz, Ewa Jamroz, Mateusz Ciszek, Ewa Emich-Widera, Marek Kijonka, Tomasz Banasik, Agnieszka Skorupa, Maria Sokół
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1da03ec3f484f2f9063765b5714d234
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spelling	oai:doaj.org-article:b1da03ec3f484f2f9063765b5714d2342021-12-02T16:08:16ZNMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results10.1038/s41598-019-51337-z2045-2322https://doaj.org/article/b1da03ec3f484f2f9063765b5714d2342019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51337-zhttps://doaj.org/toc/2045-2322Abstract Epilepsy in children is the most frequent, heterogeneous and difficult to classify chronic neurologic condition with the etiology found in 35–40% of patients. Our aim is to detect the metabolic differences between the epileptic children and the children with no neurological abnormalities in order to define the metabolic background for therapy monitoring. The studied group included 28 epilepsy patients (median age 12 months) examined with a diagnostic protocol including EEG, videoEEG, 24-hour-EEG, tests for inborn errors of metabolism, chromosomal analysis and molecular study. The reference group consisted of 20 patients (median age 20 months) with no neurological symptoms, no development delay nor chronic diseases. 1H-NMR serum spectra were acquired on 400 MHz spectrometer and analyzed using multivariate and univariate approach with the application of correction for age variation. The epilepsy group was characterized by increased levels of serum N-acetyl-glycoproteins, lactate, creatine, glycine and lipids, whereas the levels of citrate were decreased as compared to the reference group. Choline, lactate, formate and dimethylsulfone were significantly correlated with age. NMR-based metabolomics could provide information on the dynamic metabolic processes in drug-resistant epilepsy yielding not only disease-specific biomarkers but also profound insights into the disease course, treatment effects or drug toxicity.Łukasz BoguszewiczEwa JamrozMateusz CiszekEwa Emich-WideraMarek KijonkaTomasz BanasikAgnieszka SkorupaMaria SokółNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Łukasz Boguszewicz Ewa Jamroz Mateusz Ciszek Ewa Emich-Widera Marek Kijonka Tomasz Banasik Agnieszka Skorupa Maria Sokół NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results
description	Abstract Epilepsy in children is the most frequent, heterogeneous and difficult to classify chronic neurologic condition with the etiology found in 35–40% of patients. Our aim is to detect the metabolic differences between the epileptic children and the children with no neurological abnormalities in order to define the metabolic background for therapy monitoring. The studied group included 28 epilepsy patients (median age 12 months) examined with a diagnostic protocol including EEG, videoEEG, 24-hour-EEG, tests for inborn errors of metabolism, chromosomal analysis and molecular study. The reference group consisted of 20 patients (median age 20 months) with no neurological symptoms, no development delay nor chronic diseases. 1H-NMR serum spectra were acquired on 400 MHz spectrometer and analyzed using multivariate and univariate approach with the application of correction for age variation. The epilepsy group was characterized by increased levels of serum N-acetyl-glycoproteins, lactate, creatine, glycine and lipids, whereas the levels of citrate were decreased as compared to the reference group. Choline, lactate, formate and dimethylsulfone were significantly correlated with age. NMR-based metabolomics could provide information on the dynamic metabolic processes in drug-resistant epilepsy yielding not only disease-specific biomarkers but also profound insights into the disease course, treatment effects or drug toxicity.
format	article
author	Łukasz Boguszewicz Ewa Jamroz Mateusz Ciszek Ewa Emich-Widera Marek Kijonka Tomasz Banasik Agnieszka Skorupa Maria Sokół
author_facet	Łukasz Boguszewicz Ewa Jamroz Mateusz Ciszek Ewa Emich-Widera Marek Kijonka Tomasz Banasik Agnieszka Skorupa Maria Sokół
author_sort	Łukasz Boguszewicz
title	NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results
title_short	NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results
title_full	NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results
title_fullStr	NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results
title_full_unstemmed	NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results
title_sort	nmr-based metabolomics in pediatric drug resistant epilepsy – preliminary results
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b1da03ec3f484f2f9063765b5714d234
work_keys_str_mv	AT łukaszboguszewicz nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults AT ewajamroz nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults AT mateuszciszek nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults AT ewaemichwidera nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults AT marekkijonka nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults AT tomaszbanasik nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults AT agnieszkaskorupa nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults AT mariasokoł nmrbasedmetabolomicsinpediatricdrugresistantepilepsypreliminaryresults
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NMR-based metabolomics in pediatric drug resistant epilepsy – preliminary results

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