Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2

Maria Luisa Bond&igrave;1,*, Giovanna Montana2,*, Emanuela Fabiola Craparo3, Roberto Di Ges&ugrave;3, Gaetano Giammona3, Angela Bonura2, Paolo Colombo21Istituto per lo Studio dei Materiali Nanostrutturati, 2Istituto di Biomedicina ed Immunologia Molecolare, Consiglio Nazionale delle...

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Autores principales:	Bond&igrave; ML, Montana G, Craparo EF, Di Ges&ugrave; R, Giammona G, Bonura A, Colombo P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b1dd68e201d84f909aa0c3b9ad70a85e
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spelling	oai:doaj.org-article:b1dd68e201d84f909aa0c3b9ad70a85e2021-12-02T04:59:17ZLipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 21176-91141178-2013https://doaj.org/article/b1dd68e201d84f909aa0c3b9ad70a85e2011-11-01T00:00:00Zhttp://www.dovepress.com/lipid-nanoparticles-as-delivery-vehicles-for-the-parietaria-judaica-ma-a8696https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Maria Luisa Bond&igrave;1,, Giovanna Montana2,, Emanuela Fabiola Craparo3, Roberto Di Ges&ugrave;3, Gaetano Giammona3, Angela Bonura2, Paolo Colombo21Istituto per lo Studio dei Materiali Nanostrutturati, 2Istituto di Biomedicina ed Immunologia Molecolare, Consiglio Nazionale delle Ricerche, 3Laboratory of Biocompatible Polymers, Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari Stembio, Universit&agrave; di Palermo, Palermo, Italy *These authors contributed equally to this workAbstract: Parietaria pollen is one of the major causes of allergic reaction in southern Europe, affecting about 30% of all allergic patients in this area. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease by restoring a normal immunity against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was expressed in a large amount in Escherichia coli and purified to homogeneity. Its immunological properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition. Solid lipid nanoparticles were obtained by water-in-oil-in-water multiple emulsion method and characterized in terms of mean size and surface charge. These systems (approximately 250 nm diameter and negative surface charge) incorporated recombinant Par j 2 with 40% or greater efficiency. Moreover, the endotoxin level and anaphylactic activity of the empty solid lipid nanoparticles and recombinant Par j 2-loaded solid lipid nanoparticles were evaluated by looking at the overexpression of CD203c marker on human basophils. These results demonstrate that recombinant Par j 2-nanoparticles could be proposed as safe compositions for the development of new therapeutic dosage forms to cure allergic reactions.Keywords: solid lipid nanoparticles, Parietaria judaica (Par j), drug delivery, recombinant allergens, specific immunotherapy, allergic rhinitisBond&igrave; MLMontana GCraparo EFDi Ges&ugrave; RGiammona GBonura AColombo PDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2953-2962 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Bond&igrave; ML Montana G Craparo EF Di Ges&ugrave; R Giammona G Bonura A Colombo P Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2
description	Maria Luisa Bond&igrave;1,, Giovanna Montana2,, Emanuela Fabiola Craparo3, Roberto Di Ges&ugrave;3, Gaetano Giammona3, Angela Bonura2, Paolo Colombo21Istituto per lo Studio dei Materiali Nanostrutturati, 2Istituto di Biomedicina ed Immunologia Molecolare, Consiglio Nazionale delle Ricerche, 3Laboratory of Biocompatible Polymers, Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari Stembio, Universit&agrave; di Palermo, Palermo, Italy *These authors contributed equally to this workAbstract: Parietaria pollen is one of the major causes of allergic reaction in southern Europe, affecting about 30% of all allergic patients in this area. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease by restoring a normal immunity against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was expressed in a large amount in Escherichia coli and purified to homogeneity. Its immunological properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition. Solid lipid nanoparticles were obtained by water-in-oil-in-water multiple emulsion method and characterized in terms of mean size and surface charge. These systems (approximately 250 nm diameter and negative surface charge) incorporated recombinant Par j 2 with 40% or greater efficiency. Moreover, the endotoxin level and anaphylactic activity of the empty solid lipid nanoparticles and recombinant Par j 2-loaded solid lipid nanoparticles were evaluated by looking at the overexpression of CD203c marker on human basophils. These results demonstrate that recombinant Par j 2-nanoparticles could be proposed as safe compositions for the development of new therapeutic dosage forms to cure allergic reactions.Keywords: solid lipid nanoparticles, Parietaria judaica (Par j), drug delivery, recombinant allergens, specific immunotherapy, allergic rhinitis
format	article
author	Bond&igrave; ML Montana G Craparo EF Di Ges&ugrave; R Giammona G Bonura A Colombo P
author_facet	Bond&igrave; ML Montana G Craparo EF Di Ges&ugrave; R Giammona G Bonura A Colombo P
author_sort	Bond&igrave; ML
title	Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2
title_short	Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2
title_full	Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2
title_fullStr	Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2
title_full_unstemmed	Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2
title_sort	lipid nanoparticles as delivery vehicles for the parietaria judaica major allergen par j 2
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/b1dd68e201d84f909aa0c3b9ad70a85e
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Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2

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