Selective Tropism of Dengue Virus for Human Glycoprotein Ib

Abstract Since the hemorrhage in severe dengue seems to be primarily related to the defect of the platelet, the possibility that dengue virus (DENV) is selectively tropic for one of its surface receptors was investigated. Flow cytometric data of DENV-infected megakaryocytic cell line superficially e...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nattapol Attatippaholkun, Nont Kosaisawe, Yaowalak U-Pratya, Panthipa Supraditaporn, Chanchao Lorthongpanich, Kovit Pattanapanyasat, Surapol Issaragrisil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1e3089262e64129b89fc3fba2395319
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b1e3089262e64129b89fc3fba2395319
record_format	dspace
spelling	oai:doaj.org-article:b1e3089262e64129b89fc3fba23953192021-12-02T11:41:03ZSelective Tropism of Dengue Virus for Human Glycoprotein Ib10.1038/s41598-018-20914-z2045-2322https://doaj.org/article/b1e3089262e64129b89fc3fba23953192018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20914-zhttps://doaj.org/toc/2045-2322Abstract Since the hemorrhage in severe dengue seems to be primarily related to the defect of the platelet, the possibility that dengue virus (DENV) is selectively tropic for one of its surface receptors was investigated. Flow cytometric data of DENV-infected megakaryocytic cell line superficially expressing human glycoprotein Ib (CD42b) and glycoprotein IIb/IIIa (CD41 and CD41a) were analyzed by our custom-written software in MATLAB. In two-dimensional analyses, intracellular DENV was detected in CD42b+, CD41+ and CD41a+ cells. In three-dimensional analyses, the DENV was exclusively detected in CD42b+ cells but not in CD42b− cells regardless of the other expressions. In single-cell virus-protein analyses, the amount of DENV was directly correlated with those of CD42b at the Pearson correlation coefficient of 0.9. Moreover, RT- PCR and apoptosis assays showed that DENV was able to replicate itself and release its new progeny from the infected CD42b+ cells and eventually killed those cells. These results provide evidence for the involvement of CD42b in DENV infection.Nattapol AttatippaholkunNont KosaisaweYaowalak U-PratyaPanthipa SupraditapornChanchao LorthongpanichKovit PattanapanyasatSurapol IssaragrisilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nattapol Attatippaholkun Nont Kosaisawe Yaowalak U-Pratya Panthipa Supraditaporn Chanchao Lorthongpanich Kovit Pattanapanyasat Surapol Issaragrisil Selective Tropism of Dengue Virus for Human Glycoprotein Ib
description	Abstract Since the hemorrhage in severe dengue seems to be primarily related to the defect of the platelet, the possibility that dengue virus (DENV) is selectively tropic for one of its surface receptors was investigated. Flow cytometric data of DENV-infected megakaryocytic cell line superficially expressing human glycoprotein Ib (CD42b) and glycoprotein IIb/IIIa (CD41 and CD41a) were analyzed by our custom-written software in MATLAB. In two-dimensional analyses, intracellular DENV was detected in CD42b+, CD41+ and CD41a+ cells. In three-dimensional analyses, the DENV was exclusively detected in CD42b+ cells but not in CD42b− cells regardless of the other expressions. In single-cell virus-protein analyses, the amount of DENV was directly correlated with those of CD42b at the Pearson correlation coefficient of 0.9. Moreover, RT- PCR and apoptosis assays showed that DENV was able to replicate itself and release its new progeny from the infected CD42b+ cells and eventually killed those cells. These results provide evidence for the involvement of CD42b in DENV infection.
format	article
author	Nattapol Attatippaholkun Nont Kosaisawe Yaowalak U-Pratya Panthipa Supraditaporn Chanchao Lorthongpanich Kovit Pattanapanyasat Surapol Issaragrisil
author_facet	Nattapol Attatippaholkun Nont Kosaisawe Yaowalak U-Pratya Panthipa Supraditaporn Chanchao Lorthongpanich Kovit Pattanapanyasat Surapol Issaragrisil
author_sort	Nattapol Attatippaholkun
title	Selective Tropism of Dengue Virus for Human Glycoprotein Ib
title_short	Selective Tropism of Dengue Virus for Human Glycoprotein Ib
title_full	Selective Tropism of Dengue Virus for Human Glycoprotein Ib
title_fullStr	Selective Tropism of Dengue Virus for Human Glycoprotein Ib
title_full_unstemmed	Selective Tropism of Dengue Virus for Human Glycoprotein Ib
title_sort	selective tropism of dengue virus for human glycoprotein ib
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b1e3089262e64129b89fc3fba2395319
work_keys_str_mv	AT nattapolattatippaholkun selectivetropismofdenguevirusforhumanglycoproteinib AT nontkosaisawe selectivetropismofdenguevirusforhumanglycoproteinib AT yaowalakupratya selectivetropismofdenguevirusforhumanglycoproteinib AT panthipasupraditaporn selectivetropismofdenguevirusforhumanglycoproteinib AT chanchaolorthongpanich selectivetropismofdenguevirusforhumanglycoproteinib AT kovitpattanapanyasat selectivetropismofdenguevirusforhumanglycoproteinib AT surapolissaragrisil selectivetropismofdenguevirusforhumanglycoproteinib
_version_	1718395480110530560

Selective Tropism of Dengue Virus for Human Glycoprotein Ib

Ejemplares similares