Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity

D Prakash, J BehariSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi, IndiaAbstract: The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis ind...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	D Prakash, J Behari
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b1eaa3383bec47d79415f6043608019f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b1eaa3383bec47d79415f6043608019f
record_format	dspace
spelling	oai:doaj.org-article:b1eaa3383bec47d79415f6043608019f2021-12-02T02:42:17ZSynergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity1176-91141178-2013https://doaj.org/article/b1eaa3383bec47d79415f6043608019f2009-07-01T00:00:00Zhttp://www.dovepress.com/synergistic-role-of-hydroxyapatite-nanoparticles-and-pulsed-electromag-a3383https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013D Prakash, J BehariSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi, IndiaAbstract: The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We used the hind-limb suspension (HLS) rat model to simulate microgravity-induced bone losses for 45 days. In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted. As a countermeasure to the above, the effect of PEMF and HAp application were examined. Three-month-old female Wistar rats were randomly divided into control (n = 8), HLS (n = 8), HLS with PEMF (n = 8), HLS with HAp nanoparticles (n = 8), and HLS with HAp and PEMF (n = 8). We observed: 1) significant decrease (p &lt; 0.01) in BMD, Ca, P, type I collagen, and ALP activity in femur and tibia in hind-limb bone and serum osteocalcin in HLS rats as compared with the ground control. 2) Nonsignificant increase in BMD (p &lt; 0.1), Ca (p &lt; 0.1), P (p &lt; 0.5), type I collagen (p &lt; 0.1), and ALP activity (p &lt; 0.5) in femur and tibia in hind-limb bone and serum osteocalcin (p &lt; 0.5) in HLS + PEMF rats compared with HLS rats. 3) Significant increase in BMD (p &lt; 0.02), Ca (p &lt; 0.05), P (p &lt; 0.05), type I collagen (p &lt; 0.02), and ALP activity (p &gt; 0.02) in femur and tibia in hind-limb bone with a nonsignificant increase in serum osteocalcin (p &gt; 0.1) in HLS + HAp rats compared to HLS rats. 4) Significant increase in BMD (p &gt; 0.01). Ca (p &gt; 0.01). P (p &gt; 0.01). type I collagen (p &gt; 0.01). and ALP activity (p &gt; 0.01) in femur and tibia in hind-limb bone and serum osteocalcin (p &gt; 0.02) were also observed. Results suggest that a combination of low level PEMF and Hap nanoparticles has potential to control bone loss induced by simulated microgravity.Keywords: hydroxyapatite nanoparticles, simulated microgravity, osteocalcin, type I collagen D PrakashJ BehariDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 133-144 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 D Prakash J Behari Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity
description	D Prakash, J BehariSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi, IndiaAbstract: The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We used the hind-limb suspension (HLS) rat model to simulate microgravity-induced bone losses for 45 days. In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted. As a countermeasure to the above, the effect of PEMF and HAp application were examined. Three-month-old female Wistar rats were randomly divided into control (n = 8), HLS (n = 8), HLS with PEMF (n = 8), HLS with HAp nanoparticles (n = 8), and HLS with HAp and PEMF (n = 8). We observed: 1) significant decrease (p &lt; 0.01) in BMD, Ca, P, type I collagen, and ALP activity in femur and tibia in hind-limb bone and serum osteocalcin in HLS rats as compared with the ground control. 2) Nonsignificant increase in BMD (p &lt; 0.1), Ca (p &lt; 0.1), P (p &lt; 0.5), type I collagen (p &lt; 0.1), and ALP activity (p &lt; 0.5) in femur and tibia in hind-limb bone and serum osteocalcin (p &lt; 0.5) in HLS + PEMF rats compared with HLS rats. 3) Significant increase in BMD (p &lt; 0.02), Ca (p &lt; 0.05), P (p &lt; 0.05), type I collagen (p &lt; 0.02), and ALP activity (p &gt; 0.02) in femur and tibia in hind-limb bone with a nonsignificant increase in serum osteocalcin (p &gt; 0.1) in HLS + HAp rats compared to HLS rats. 4) Significant increase in BMD (p &gt; 0.01). Ca (p &gt; 0.01). P (p &gt; 0.01). type I collagen (p &gt; 0.01). and ALP activity (p &gt; 0.01) in femur and tibia in hind-limb bone and serum osteocalcin (p &gt; 0.02) were also observed. Results suggest that a combination of low level PEMF and Hap nanoparticles has potential to control bone loss induced by simulated microgravity.Keywords: hydroxyapatite nanoparticles, simulated microgravity, osteocalcin, type I collagen
format	article
author	D Prakash J Behari
author_facet	D Prakash J Behari
author_sort	D Prakash
title	Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity
title_short	Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity
title_full	Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity
title_fullStr	Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity
title_full_unstemmed	Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity
title_sort	synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/b1eaa3383bec47d79415f6043608019f
work_keys_str_mv	AT dprakash synergisticroleofhydroxyapatitenanoparticlesandpulsedelectromagneticfieldtherapytopreventbonelossinratsfollowingexposuretosimulatedmicrogravity AT jbehari synergisticroleofhydroxyapatitenanoparticlesandpulsedelectromagneticfieldtherapytopreventbonelossinratsfollowingexposuretosimulatedmicrogravity
_version_	1718402245626691584

Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity

Ejemplares similares