Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits

The fruit of Melothria scabra Naudin is traditionally used by natives of South East Sulawesi and has economic values in the local markets. Nonetheless, little scientific information was gained from this plant to support its development for nutraceutical and pharmaceutical aspects. This study aimed t...

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Autores principales:	Harni Sartika Kamaruddin, Megawati Megawati, Nurliana Nurliana, Carla Wulandari Sabandar
Formato:	article
Lenguaje:	EN ID
Publicado:	Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya 2021
Materias:	Cucurbitaceae Melothria scabra Naudin Cucurbitacin B glycoside Antioxidant activity Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/b1ead6f6202443a388108e354b20666e
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spelling	oai:doaj.org-article:b1ead6f6202443a388108e354b20666e2021-12-01T04:43:49ZChemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits10.33084/bjop.v4i4.28902621-4814https://doaj.org/article/b1ead6f6202443a388108e354b20666e2021-11-01T00:00:00Zhttp://journal.umpalangkaraya.ac.id/index.php/bjop/article/view/2890https://doaj.org/toc/2621-4814The fruit of Melothria scabra Naudin is traditionally used by natives of South East Sulawesi and has economic values in the local markets. Nonetheless, little scientific information was gained from this plant to support its development for nutraceutical and pharmaceutical aspects. This study aimed to investigate the phytochemicals contained in the ethanol extract and organic fractions (methanol, ethyl acetate, and hexane) of the fruits using specific reagents and an LC-MS/MS analysis, as well as to evaluate their total phenolics, total flavonoids, and DPPH radical scavenging activity using a dot-blot staining and spectrophotometric assays. Results showed that the fruits of M. scabra contained alkaloids, tannins, flavonoids, terpenoids, and saponins. Six compounds were successfully identified from the ethanol extract of the fruits for the first time that is D-1-[(3-carboxypropyl)amino]-1-deoxyfructose (1), fructose-C3H5NO (2), valine (3), 1β, 3α, 9β-trihydroxyeudesma-5,11(13)-dien-12-oic acid (4), Cucurbitacin B-2-O-α-L-rhamnopyranosyl-β-D-glucopyranoside (5), and 2-heptyl-3-hydroxy-4(H)-quinolone (6). Total phenolics in the extract and organic fractions were in the range of 54.2 ± 2.4 to 259.1 ± 8.4 mg GAE/g, while total flavonoids were in the range of 1.6 ± 0.2 to 22.4 ± 0.2 mg QE/g. The ethanol extract and its organic fractions (methanol and ethyl acetate) were potent radical scavengers with SC50 values ranging from 20.7 to 37.5 µg/mL when compared with ascorbic acid, gallic acid, and quercetin (SC50s 2.8 to 9.4 µg/mL). This study concludes that M. scabra fruits could be developed as a source of natural antioxidant agents for nutraceuticals and pharmaceuticals purposes. Harni Sartika KamaruddinMegawati MegawatiNurliana NurlianaCarla Wulandari SabandarInstitute for Researches and Community Services Universitas Muhammadiyah Palangkaraya articleCucurbitaceaeMelothria scabra NaudinCucurbitacin B glycosideAntioxidant activityPharmacy and materia medicaRS1-441ENIDBorneo Journal of Pharmacy, Vol 4, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Cucurbitaceae Melothria scabra Naudin Cucurbitacin B glycoside Antioxidant activity Pharmacy and materia medica RS1-441
spellingShingle	Cucurbitaceae Melothria scabra Naudin Cucurbitacin B glycoside Antioxidant activity Pharmacy and materia medica RS1-441 Harni Sartika Kamaruddin Megawati Megawati Nurliana Nurliana Carla Wulandari Sabandar Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits
description	The fruit of Melothria scabra Naudin is traditionally used by natives of South East Sulawesi and has economic values in the local markets. Nonetheless, little scientific information was gained from this plant to support its development for nutraceutical and pharmaceutical aspects. This study aimed to investigate the phytochemicals contained in the ethanol extract and organic fractions (methanol, ethyl acetate, and hexane) of the fruits using specific reagents and an LC-MS/MS analysis, as well as to evaluate their total phenolics, total flavonoids, and DPPH radical scavenging activity using a dot-blot staining and spectrophotometric assays. Results showed that the fruits of M. scabra contained alkaloids, tannins, flavonoids, terpenoids, and saponins. Six compounds were successfully identified from the ethanol extract of the fruits for the first time that is D-1-[(3-carboxypropyl)amino]-1-deoxyfructose (1), fructose-C3H5NO (2), valine (3), 1β, 3α, 9β-trihydroxyeudesma-5,11(13)-dien-12-oic acid (4), Cucurbitacin B-2-O-α-L-rhamnopyranosyl-β-D-glucopyranoside (5), and 2-heptyl-3-hydroxy-4(H)-quinolone (6). Total phenolics in the extract and organic fractions were in the range of 54.2 ± 2.4 to 259.1 ± 8.4 mg GAE/g, while total flavonoids were in the range of 1.6 ± 0.2 to 22.4 ± 0.2 mg QE/g. The ethanol extract and its organic fractions (methanol and ethyl acetate) were potent radical scavengers with SC50 values ranging from 20.7 to 37.5 µg/mL when compared with ascorbic acid, gallic acid, and quercetin (SC50s 2.8 to 9.4 µg/mL). This study concludes that M. scabra fruits could be developed as a source of natural antioxidant agents for nutraceuticals and pharmaceuticals purposes.
format	article
author	Harni Sartika Kamaruddin Megawati Megawati Nurliana Nurliana Carla Wulandari Sabandar
author_facet	Harni Sartika Kamaruddin Megawati Megawati Nurliana Nurliana Carla Wulandari Sabandar
author_sort	Harni Sartika Kamaruddin
title	Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits
title_short	Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits
title_full	Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits
title_fullStr	Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits
title_full_unstemmed	Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits
title_sort	chemical constituents and antioxidant activity of melothria scabra naudin fruits
publisher	Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya
publishDate	2021
url	https://doaj.org/article/b1ead6f6202443a388108e354b20666e
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Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits

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