Insulin micro-secretion in Type 1 diabetes and related microRNA profiles

Abstract The aim of this cross-sectional study was to compare plasma C-peptide presence and levels in people without diabetes (CON) and with Type 1 diabetes and relate C-peptide status to clinical factors. In a subset we evaluated 50 microRNAs (miRs) previously implicated in beta-cell death and asso...

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Autores principales:	Andrzej S. Januszewski, Yoon Hi Cho, Mugdha V. Joglekar, Ryan J. Farr, Emma S. Scott, Wilson K. M. Wong, Luke M. Carroll, Yik W. Loh, Paul Z. Benitez-Aguirre, Anthony C. Keech, David N. O’Neal, Maria E. Craig, Anandwardhan A. Hardikar, Kim C. Donaghue, Alicia J. Jenkins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1ec56fdb12a4c0cb5cb32be78d2f006
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spelling	oai:doaj.org-article:b1ec56fdb12a4c0cb5cb32be78d2f0062021-12-02T17:51:21ZInsulin micro-secretion in Type 1 diabetes and related microRNA profiles10.1038/s41598-021-90856-62045-2322https://doaj.org/article/b1ec56fdb12a4c0cb5cb32be78d2f0062021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90856-6https://doaj.org/toc/2045-2322Abstract The aim of this cross-sectional study was to compare plasma C-peptide presence and levels in people without diabetes (CON) and with Type 1 diabetes and relate C-peptide status to clinical factors. In a subset we evaluated 50 microRNAs (miRs) previously implicated in beta-cell death and associations with clinical status and C-peptide levels. Diabetes age of onset was stratified as adult (≥ 18 y.o) or childhood (< 18 y.o.), and diabetes duration was stratified as ≤ 10 years, 10–20 years and > 20 years. Plasma C-peptide was measured by ultrasensitive ELISA. Plasma miRs were quantified using TaqMan probe-primer mix on an OpenArray platform. C-peptide was detectable in 55.3% of (n = 349) people with diabetes, including 64.1% of adults and 34.0% of youth with diabetes, p < 0.0001 and in all (n = 253) participants without diabetes (CON). C-peptide levels, when detectable, were lower in the individuals with diabetes than in the CON group [median lower quartile (LQ)–upper quartile (UQ)] 5.0 (2.6–28.7) versus 650.9 (401.2–732.4) pmol/L respectively, p < 0.0001 and lower in childhood versus adult-onset diabetes [median (LQ–UQ) 4.2 (2.6–12.2) pmol/L vs. 8.0 (2.3–80.5) pmol/L, p = 0.02, respectively]. In the childhood-onset group more people with longer diabetes duration (> 20 years) had detectable C-peptide (60%) than in those with shorter diabetes duration (39%, p for trend < 0.05). Nine miRs significantly correlated with detectable C-peptide levels in people with diabetes and 16 miRs correlated with C-peptide levels in CON. Our cross-sectional study results are supportive of (a) greater beta-cell function loss in younger onset Type 1 diabetes; (b) persistent insulin secretion in adult-onset diabetes and possibly regenerative secretion in childhood-onset long diabetes duration; and (c) relationships of C-peptide levels with circulating miRs. Confirmatory clinical studies and related basic science studies are merited.Andrzej S. JanuszewskiYoon Hi ChoMugdha V. JoglekarRyan J. FarrEmma S. ScottWilson K. M. WongLuke M. CarrollYik W. LohPaul Z. Benitez-AguirreAnthony C. KeechDavid N. O’NealMaria E. CraigAnandwardhan A. HardikarKim C. DonaghueAlicia J. JenkinsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrzej S. Januszewski Yoon Hi Cho Mugdha V. Joglekar Ryan J. Farr Emma S. Scott Wilson K. M. Wong Luke M. Carroll Yik W. Loh Paul Z. Benitez-Aguirre Anthony C. Keech David N. O’Neal Maria E. Craig Anandwardhan A. Hardikar Kim C. Donaghue Alicia J. Jenkins Insulin micro-secretion in Type 1 diabetes and related microRNA profiles
description	Abstract The aim of this cross-sectional study was to compare plasma C-peptide presence and levels in people without diabetes (CON) and with Type 1 diabetes and relate C-peptide status to clinical factors. In a subset we evaluated 50 microRNAs (miRs) previously implicated in beta-cell death and associations with clinical status and C-peptide levels. Diabetes age of onset was stratified as adult (≥ 18 y.o) or childhood (< 18 y.o.), and diabetes duration was stratified as ≤ 10 years, 10–20 years and > 20 years. Plasma C-peptide was measured by ultrasensitive ELISA. Plasma miRs were quantified using TaqMan probe-primer mix on an OpenArray platform. C-peptide was detectable in 55.3% of (n = 349) people with diabetes, including 64.1% of adults and 34.0% of youth with diabetes, p < 0.0001 and in all (n = 253) participants without diabetes (CON). C-peptide levels, when detectable, were lower in the individuals with diabetes than in the CON group [median lower quartile (LQ)–upper quartile (UQ)] 5.0 (2.6–28.7) versus 650.9 (401.2–732.4) pmol/L respectively, p < 0.0001 and lower in childhood versus adult-onset diabetes [median (LQ–UQ) 4.2 (2.6–12.2) pmol/L vs. 8.0 (2.3–80.5) pmol/L, p = 0.02, respectively]. In the childhood-onset group more people with longer diabetes duration (> 20 years) had detectable C-peptide (60%) than in those with shorter diabetes duration (39%, p for trend < 0.05). Nine miRs significantly correlated with detectable C-peptide levels in people with diabetes and 16 miRs correlated with C-peptide levels in CON. Our cross-sectional study results are supportive of (a) greater beta-cell function loss in younger onset Type 1 diabetes; (b) persistent insulin secretion in adult-onset diabetes and possibly regenerative secretion in childhood-onset long diabetes duration; and (c) relationships of C-peptide levels with circulating miRs. Confirmatory clinical studies and related basic science studies are merited.
format	article
author	Andrzej S. Januszewski Yoon Hi Cho Mugdha V. Joglekar Ryan J. Farr Emma S. Scott Wilson K. M. Wong Luke M. Carroll Yik W. Loh Paul Z. Benitez-Aguirre Anthony C. Keech David N. O’Neal Maria E. Craig Anandwardhan A. Hardikar Kim C. Donaghue Alicia J. Jenkins
author_facet	Andrzej S. Januszewski Yoon Hi Cho Mugdha V. Joglekar Ryan J. Farr Emma S. Scott Wilson K. M. Wong Luke M. Carroll Yik W. Loh Paul Z. Benitez-Aguirre Anthony C. Keech David N. O’Neal Maria E. Craig Anandwardhan A. Hardikar Kim C. Donaghue Alicia J. Jenkins
author_sort	Andrzej S. Januszewski
title	Insulin micro-secretion in Type 1 diabetes and related microRNA profiles
title_short	Insulin micro-secretion in Type 1 diabetes and related microRNA profiles
title_full	Insulin micro-secretion in Type 1 diabetes and related microRNA profiles
title_fullStr	Insulin micro-secretion in Type 1 diabetes and related microRNA profiles
title_full_unstemmed	Insulin micro-secretion in Type 1 diabetes and related microRNA profiles
title_sort	insulin micro-secretion in type 1 diabetes and related microrna profiles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b1ec56fdb12a4c0cb5cb32be78d2f006
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Insulin micro-secretion in Type 1 diabetes and related microRNA profiles

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