Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk

Abstract Cases of human gastric cancer due to Helicobacter pylori have been reported worldwide and animals might act as a reservoir of infection in certain circumstances. The recent few decades showed a rapid decline in the incidence of gastric cancer, which was mainly due to the decrease in H. pylo...

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Autores principales:	Mahmoud Elhariri, Dalia Hamza, Rehab Elhelw, Eman Hamza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Pylori Load Gastric Infection Pylori Isolates Pylori Strains Mouse Gastric Mucosa Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1f4ba2e9d714fd6841754c3aee4232f
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spelling	oai:doaj.org-article:b1f4ba2e9d714fd6841754c3aee4232f2021-12-02T15:08:48ZOccurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk10.1038/s41598-018-32671-02045-2322https://doaj.org/article/b1f4ba2e9d714fd6841754c3aee4232f2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32671-0https://doaj.org/toc/2045-2322Abstract Cases of human gastric cancer due to Helicobacter pylori have been reported worldwide and animals might act as a reservoir of infection in certain circumstances. The recent few decades showed a rapid decline in the incidence of gastric cancer, which was mainly due to the decrease in H. pylori infection. The aims of the present study were to determine the prevalence of H. pylori among livestock and investigate whether the animal isolates can be transmitted through contaminated milk causing gastric infection. Feces and milk samples were collected from apparently healthy cows, buffaloes, and sheep, and were examined by nested PCR and genotyping. The PCR positive samples were further subjected to bacterial culture followed by partial 16s sequencing of the isolates. Twenty-nine percent of the animals showed the presence of H. pylori, mainly the virulent cagA + vacA + s1a m1 i1 genotype, which is known to be associated with serious diseases in humans. The spiral viable culturable form (SVCF) of this strain was inoculated into UHT (ultra-high temperature) milk and remained viable for up to 10 days at 4 °C. Increasing period of storage and or temperature led to a decrease in the number of the SVCF and occurrence of the coccoid viable non-culturable form (CVNCF). The infectivity of the survived forms was determined by feeding healthy groups of laboratory mice with the contaminated UHT milk containing SVCF or CVNCF for 40 days. The gastric mucosa of the two mice groups showed similar levels of H. pylori load. This highlights that H. pylori can persist in contaminated milk by entering a non-culturable state, which can induce gastric infection.Mahmoud ElhaririDalia HamzaRehab ElhelwEman HamzaNature PortfolioarticlePylori LoadGastric InfectionPylori IsolatesPylori StrainsMouse Gastric MucosaMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pylori Load Gastric Infection Pylori Isolates Pylori Strains Mouse Gastric Mucosa Medicine R Science Q
spellingShingle	Pylori Load Gastric Infection Pylori Isolates Pylori Strains Mouse Gastric Mucosa Medicine R Science Q Mahmoud Elhariri Dalia Hamza Rehab Elhelw Eman Hamza Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk
description	Abstract Cases of human gastric cancer due to Helicobacter pylori have been reported worldwide and animals might act as a reservoir of infection in certain circumstances. The recent few decades showed a rapid decline in the incidence of gastric cancer, which was mainly due to the decrease in H. pylori infection. The aims of the present study were to determine the prevalence of H. pylori among livestock and investigate whether the animal isolates can be transmitted through contaminated milk causing gastric infection. Feces and milk samples were collected from apparently healthy cows, buffaloes, and sheep, and were examined by nested PCR and genotyping. The PCR positive samples were further subjected to bacterial culture followed by partial 16s sequencing of the isolates. Twenty-nine percent of the animals showed the presence of H. pylori, mainly the virulent cagA + vacA + s1a m1 i1 genotype, which is known to be associated with serious diseases in humans. The spiral viable culturable form (SVCF) of this strain was inoculated into UHT (ultra-high temperature) milk and remained viable for up to 10 days at 4 °C. Increasing period of storage and or temperature led to a decrease in the number of the SVCF and occurrence of the coccoid viable non-culturable form (CVNCF). The infectivity of the survived forms was determined by feeding healthy groups of laboratory mice with the contaminated UHT milk containing SVCF or CVNCF for 40 days. The gastric mucosa of the two mice groups showed similar levels of H. pylori load. This highlights that H. pylori can persist in contaminated milk by entering a non-culturable state, which can induce gastric infection.
format	article
author	Mahmoud Elhariri Dalia Hamza Rehab Elhelw Eman Hamza
author_facet	Mahmoud Elhariri Dalia Hamza Rehab Elhelw Eman Hamza
author_sort	Mahmoud Elhariri
title	Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk
title_short	Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk
title_full	Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk
title_fullStr	Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk
title_full_unstemmed	Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk
title_sort	occurrence of caga + vaca s1a m1 i1 helicobacter pylori in farm animals in egypt and ability to survive in experimentally contaminated uht milk
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b1f4ba2e9d714fd6841754c3aee4232f
work_keys_str_mv	AT mahmoudelhariri occurrenceofcagavacas1am1i1helicobacterpyloriinfarmanimalsinegyptandabilitytosurviveinexperimentallycontaminateduhtmilk AT daliahamza occurrenceofcagavacas1am1i1helicobacterpyloriinfarmanimalsinegyptandabilitytosurviveinexperimentallycontaminateduhtmilk AT rehabelhelw occurrenceofcagavacas1am1i1helicobacterpyloriinfarmanimalsinegyptandabilitytosurviveinexperimentallycontaminateduhtmilk AT emanhamza occurrenceofcagavacas1am1i1helicobacterpyloriinfarmanimalsinegyptandabilitytosurviveinexperimentallycontaminateduhtmilk
_version_	1718388021336735744

Occurrence of cagA + vacA s1a m1 i1 Helicobacter pylori in farm animals in Egypt and ability to survive in experimentally contaminated UHT milk

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