Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain

Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneum...

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Autores principales:	Xuemei Yang, Ning Dong, Xiaoxuan Liu, Chen Yang, Lianwei Ye, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Klebsiella pneumonia conjugative virulence plasmid blaKPC-bearing plasmid transmission CR-HvKP IncFIB/IncHI1B Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b1f4cce6b7a74d5e9e89d27da48dfb5e
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spelling	oai:doaj.org-article:b1f4cce6b7a74d5e9e89d27da48dfb5e2021-11-08T07:21:58ZCo-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain1664-302X10.3389/fmicb.2021.739461https://doaj.org/article/b1f4cce6b7a74d5e9e89d27da48dfb5e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.739461/fullhttps://doaj.org/toc/1664-302XCarbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type blaKPC–2-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the blaKPC–2-bearing plasmid. Co-transmission of virulence and blaKPC–2-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health.Xuemei YangNing DongXiaoxuan LiuChen YangLianwei YeEdward Wai-Chi ChanEdward Wai-Chi ChanRong ZhangSheng ChenFrontiers Media S.A.articleKlebsiella pneumoniaconjugative virulence plasmidblaKPC-bearing plasmidtransmissionCR-HvKPIncFIB/IncHI1BMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Klebsiella pneumonia conjugative virulence plasmid blaKPC-bearing plasmid transmission CR-HvKP IncFIB/IncHI1B Microbiology QR1-502
spellingShingle	Klebsiella pneumonia conjugative virulence plasmid blaKPC-bearing plasmid transmission CR-HvKP IncFIB/IncHI1B Microbiology QR1-502 Xuemei Yang Ning Dong Xiaoxuan Liu Chen Yang Lianwei Ye Edward Wai-Chi Chan Edward Wai-Chi Chan Rong Zhang Sheng Chen Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain
description	Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type blaKPC–2-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the blaKPC–2-bearing plasmid. Co-transmission of virulence and blaKPC–2-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health.
format	article
author	Xuemei Yang Ning Dong Xiaoxuan Liu Chen Yang Lianwei Ye Edward Wai-Chi Chan Edward Wai-Chi Chan Rong Zhang Sheng Chen
author_facet	Xuemei Yang Ning Dong Xiaoxuan Liu Chen Yang Lianwei Ye Edward Wai-Chi Chan Edward Wai-Chi Chan Rong Zhang Sheng Chen
author_sort	Xuemei Yang
title	Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain
title_short	Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain
title_full	Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain
title_fullStr	Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain
title_full_unstemmed	Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain
title_sort	co-conjugation of virulence plasmid and kpc plasmid in a clinical klebsiella pneumoniae strain
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b1f4cce6b7a74d5e9e89d27da48dfb5e
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Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain

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