Control of virulence by small RNAs in Streptococcus pneumoniae.

Small noncoding RNAs (sRNAs) play important roles in gene regulation in both prokaryotes and eukaryotes. Thus far, no sRNA has been assigned a definitive role in virulence in the major human pathogen Streptococcus pneumoniae. Based on the potential coding capacity of intergenic regions, we hypothesi...

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Autores principales:	Beth Mann, Tim van Opijnen, Jianmin Wang, Caroline Obert, Yong-Dong Wang, Robert Carter, Daniel J McGoldrick, Granger Ridout, Andrew Camilli, Elaine I Tuomanen, Jason W Rosch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b1f6e1760cbb44c5a08d464b17d027d8
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spelling	oai:doaj.org-article:b1f6e1760cbb44c5a08d464b17d027d82021-11-18T06:04:13ZControl of virulence by small RNAs in Streptococcus pneumoniae.1553-73661553-737410.1371/journal.ppat.1002788https://doaj.org/article/b1f6e1760cbb44c5a08d464b17d027d82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22807675/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Small noncoding RNAs (sRNAs) play important roles in gene regulation in both prokaryotes and eukaryotes. Thus far, no sRNA has been assigned a definitive role in virulence in the major human pathogen Streptococcus pneumoniae. Based on the potential coding capacity of intergenic regions, we hypothesized that the pneumococcus produces many sRNAs and that they would play an important role in pathogenesis. We describe the application of whole-genome transcriptional sequencing to systematically identify the sRNAs of Streptococcus pneumoniae. Using this approach, we have identified 89 putative sRNAs, 56 of which are newly identified. Furthermore, using targeted genetic approaches and Tn-seq transposon screening, we demonstrate that many of the identified sRNAs have important global and niche-specific roles in virulence. These data constitute the most comprehensive analysis of pneumococcal sRNAs and provide the first evidence of the extensive roles of sRNAs in pneumococcal pathogenesis.Beth MannTim van OpijnenJianmin WangCaroline ObertYong-Dong WangRobert CarterDaniel J McGoldrickGranger RidoutAndrew CamilliElaine I TuomanenJason W RoschPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 7, p e1002788 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Beth Mann Tim van Opijnen Jianmin Wang Caroline Obert Yong-Dong Wang Robert Carter Daniel J McGoldrick Granger Ridout Andrew Camilli Elaine I Tuomanen Jason W Rosch Control of virulence by small RNAs in Streptococcus pneumoniae.
description	Small noncoding RNAs (sRNAs) play important roles in gene regulation in both prokaryotes and eukaryotes. Thus far, no sRNA has been assigned a definitive role in virulence in the major human pathogen Streptococcus pneumoniae. Based on the potential coding capacity of intergenic regions, we hypothesized that the pneumococcus produces many sRNAs and that they would play an important role in pathogenesis. We describe the application of whole-genome transcriptional sequencing to systematically identify the sRNAs of Streptococcus pneumoniae. Using this approach, we have identified 89 putative sRNAs, 56 of which are newly identified. Furthermore, using targeted genetic approaches and Tn-seq transposon screening, we demonstrate that many of the identified sRNAs have important global and niche-specific roles in virulence. These data constitute the most comprehensive analysis of pneumococcal sRNAs and provide the first evidence of the extensive roles of sRNAs in pneumococcal pathogenesis.
format	article
author	Beth Mann Tim van Opijnen Jianmin Wang Caroline Obert Yong-Dong Wang Robert Carter Daniel J McGoldrick Granger Ridout Andrew Camilli Elaine I Tuomanen Jason W Rosch
author_facet	Beth Mann Tim van Opijnen Jianmin Wang Caroline Obert Yong-Dong Wang Robert Carter Daniel J McGoldrick Granger Ridout Andrew Camilli Elaine I Tuomanen Jason W Rosch
author_sort	Beth Mann
title	Control of virulence by small RNAs in Streptococcus pneumoniae.
title_short	Control of virulence by small RNAs in Streptococcus pneumoniae.
title_full	Control of virulence by small RNAs in Streptococcus pneumoniae.
title_fullStr	Control of virulence by small RNAs in Streptococcus pneumoniae.
title_full_unstemmed	Control of virulence by small RNAs in Streptococcus pneumoniae.
title_sort	control of virulence by small rnas in streptococcus pneumoniae.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b1f6e1760cbb44c5a08d464b17d027d8
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Control of virulence by small RNAs in Streptococcus pneumoniae.

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