Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2

Abstract Reduction of elastin in the skin causes various skin diseases as well as wrinkles and sagging with aging. Sialidase is a hydrolase that cleaves a sialic acid residue from sialoglycoconjugate. Cleavage of sialic acid from microfibrils by the sialidase isozyme Neu1 facilitates elastic fiber a...

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Autores principales:	Akira Minami, Yuka Fujita, Jun Goto, Ayano Iuchi, Kosei Fujita, Yasuyo Mikami, Mako Shiratori, Ami Ishii, Samir Mitragotri, Yasunori Iwao, Hiroaki Kanazawa, Yuuki Kurebayashi, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b206dd55f73d4226b1ced499d9b7d30c
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spelling	oai:doaj.org-article:b206dd55f73d4226b1ced499d9b7d30c2021-12-02T14:27:01ZEnhancement of elastin expression by transdermal administration of sialidase isozyme Neu210.1038/s41598-021-82820-12045-2322https://doaj.org/article/b206dd55f73d4226b1ced499d9b7d30c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82820-1https://doaj.org/toc/2045-2322Abstract Reduction of elastin in the skin causes various skin diseases as well as wrinkles and sagging with aging. Sialidase is a hydrolase that cleaves a sialic acid residue from sialoglycoconjugate. Cleavage of sialic acid from microfibrils by the sialidase isozyme Neu1 facilitates elastic fiber assembly. In the present study, we showed that a lower layer of the dermis and muscle showed relatively intense sialidase activity. The sialidase activity in the skin decreased with aging. Choline and geranate (CAGE), one of the ionic liquids, can deliver the sialidase subcutaneously while maintaining the enzymatic activity. The elastin level in the dermis was increased by applying sialidase from Arthrobacter ureafaciens (AUSA) with CAGE on the skin for 5 days in rats and senescence-accelerated mice prone 1 and 8. Sialidase activity in the dermis was considered to be mainly due to Neu2 based on the expression level of sialidase isozyme mRNA. Transdermal administration of Neu2 with CAGE also increased the level of elastin in the dermis. Therefore, not only Neu1 but also Neu2 would be involved in elastic fiber assembly. Transdermal administration of sialidase is expected to be useful for improvement of wrinkles and skin disorders due to the loss of elastic fibers.Akira MinamiYuka FujitaJun GotoAyano IuchiKosei FujitaYasuyo MikamiMako ShiratoriAmi IshiiSamir MitragotriYasunori IwaoHiroaki KanazawaYuuki KurebayashiTadanobu TakahashiTadamune OtsuboKiyoshi IkedaTakashi SuzukiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akira Minami Yuka Fujita Jun Goto Ayano Iuchi Kosei Fujita Yasuyo Mikami Mako Shiratori Ami Ishii Samir Mitragotri Yasunori Iwao Hiroaki Kanazawa Yuuki Kurebayashi Tadanobu Takahashi Tadamune Otsubo Kiyoshi Ikeda Takashi Suzuki Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2
description	Abstract Reduction of elastin in the skin causes various skin diseases as well as wrinkles and sagging with aging. Sialidase is a hydrolase that cleaves a sialic acid residue from sialoglycoconjugate. Cleavage of sialic acid from microfibrils by the sialidase isozyme Neu1 facilitates elastic fiber assembly. In the present study, we showed that a lower layer of the dermis and muscle showed relatively intense sialidase activity. The sialidase activity in the skin decreased with aging. Choline and geranate (CAGE), one of the ionic liquids, can deliver the sialidase subcutaneously while maintaining the enzymatic activity. The elastin level in the dermis was increased by applying sialidase from Arthrobacter ureafaciens (AUSA) with CAGE on the skin for 5 days in rats and senescence-accelerated mice prone 1 and 8. Sialidase activity in the dermis was considered to be mainly due to Neu2 based on the expression level of sialidase isozyme mRNA. Transdermal administration of Neu2 with CAGE also increased the level of elastin in the dermis. Therefore, not only Neu1 but also Neu2 would be involved in elastic fiber assembly. Transdermal administration of sialidase is expected to be useful for improvement of wrinkles and skin disorders due to the loss of elastic fibers.
format	article
author	Akira Minami Yuka Fujita Jun Goto Ayano Iuchi Kosei Fujita Yasuyo Mikami Mako Shiratori Ami Ishii Samir Mitragotri Yasunori Iwao Hiroaki Kanazawa Yuuki Kurebayashi Tadanobu Takahashi Tadamune Otsubo Kiyoshi Ikeda Takashi Suzuki
author_facet	Akira Minami Yuka Fujita Jun Goto Ayano Iuchi Kosei Fujita Yasuyo Mikami Mako Shiratori Ami Ishii Samir Mitragotri Yasunori Iwao Hiroaki Kanazawa Yuuki Kurebayashi Tadanobu Takahashi Tadamune Otsubo Kiyoshi Ikeda Takashi Suzuki
author_sort	Akira Minami
title	Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2
title_short	Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2
title_full	Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2
title_fullStr	Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2
title_full_unstemmed	Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2
title_sort	enhancement of elastin expression by transdermal administration of sialidase isozyme neu2
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b206dd55f73d4226b1ced499d9b7d30c
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Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2

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