ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)

Problems of regulation of oxidative stress and the search for biologically active substances with antioxidant activity are the focus of researchers. Chicory (Cichorium intybus L.) is a plant containing various biologically active substances (hydroxycinnamic acid, flavonoids, coumarins, ascorbic acid...

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Autores principales:	O. L. Saybel, T. D. Dargaeva, K. A. Pupykina, I. V. Petrova, R. R. Farkhutdinov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	cichorium intybus l antioxidant properties chemoluminescence Science Q
Acceso en línea:	https://doaj.org/article/b20a93c9ee694c4eb4c4affbfe318ba6
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spelling	oai:doaj.org-article:b20a93c9ee694c4eb4c4affbfe318ba62021-11-23T06:14:37ZASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)2541-94202587-959610.12737/article_59a614fcd18c42.95236968https://doaj.org/article/b20a93c9ee694c4eb4c4affbfe318ba62017-04-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/625https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Problems of regulation of oxidative stress and the search for biologically active substances with antioxidant activity are the focus of researchers. Chicory (Cichorium intybus L.) is a plant containing various biologically active substances (hydroxycinnamic acid, flavonoids, coumarins, ascorbic acid, carotenoids, etc.), exhibiting antioxidant properties. The aim of our study was to evaluate the effect of aqueous and alcoholic extracts of chicory herbs on the processes of free radical oxidation in model systems in vitro using rapid method of determining antioxidant activity, based on the registration of chemiluminescence. A model system generating active oxygen species (ROS) was phosphate buffer with the addition of luminol solution and sodium citrate to initiate reactions involving the formation of ROS injected Fe2+ salt solution. Impact of extracts on lipid peroxidation was studied in chicken yolks homogenized in phosphate buffer. For initiation of oxidation reactions Fe2+ solution was used. Lipid peroxidation (LPO) processes were judged by the light intensity. The effect of aqueous and alcoholic extracts of common chicory herbs in various concentrations on the processes of free radical oxidation in the active forms of oxygen in the system (ROS) and LPO was researched. The study revealed the ability of the extracts of the chicory herb suppress the generation of ROS and LPO in model systems that characterizes their antioxidant properties, which are most pronounced when administered aqueous extracts of chicory herbs compared with alcohol in model systems at a concentration of 0.1 mg/ml.O. L. SaybelT. D. DargaevaK. A. PupykinaI. V. PetrovaR. R. FarkhutdinovScientific Сentre for Family Health and Human Reproduction Problemsarticlecichorium intybus lcichorium intybus lantioxidant propertieschemoluminescenceScienceQRUActa Biomedica Scientifica, Vol 2, Iss 2, Pp 85-88 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	cichorium intybus l cichorium intybus l antioxidant properties chemoluminescence Science Q
spellingShingle	cichorium intybus l cichorium intybus l antioxidant properties chemoluminescence Science Q O. L. Saybel T. D. Dargaeva K. A. Pupykina I. V. Petrova R. R. Farkhutdinov ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)
description	Problems of regulation of oxidative stress and the search for biologically active substances with antioxidant activity are the focus of researchers. Chicory (Cichorium intybus L.) is a plant containing various biologically active substances (hydroxycinnamic acid, flavonoids, coumarins, ascorbic acid, carotenoids, etc.), exhibiting antioxidant properties. The aim of our study was to evaluate the effect of aqueous and alcoholic extracts of chicory herbs on the processes of free radical oxidation in model systems in vitro using rapid method of determining antioxidant activity, based on the registration of chemiluminescence. A model system generating active oxygen species (ROS) was phosphate buffer with the addition of luminol solution and sodium citrate to initiate reactions involving the formation of ROS injected Fe2+ salt solution. Impact of extracts on lipid peroxidation was studied in chicken yolks homogenized in phosphate buffer. For initiation of oxidation reactions Fe2+ solution was used. Lipid peroxidation (LPO) processes were judged by the light intensity. The effect of aqueous and alcoholic extracts of common chicory herbs in various concentrations on the processes of free radical oxidation in the active forms of oxygen in the system (ROS) and LPO was researched. The study revealed the ability of the extracts of the chicory herb suppress the generation of ROS and LPO in model systems that characterizes their antioxidant properties, which are most pronounced when administered aqueous extracts of chicory herbs compared with alcohol in model systems at a concentration of 0.1 mg/ml.
format	article
author	O. L. Saybel T. D. Dargaeva K. A. Pupykina I. V. Petrova R. R. Farkhutdinov
author_facet	O. L. Saybel T. D. Dargaeva K. A. Pupykina I. V. Petrova R. R. Farkhutdinov
author_sort	O. L. Saybel
title	ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)
title_short	ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)
title_full	ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)
title_fullStr	ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)
title_full_unstemmed	ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)
title_sort	assessment of antioxidant activity of witloof chicory herb (cichorium intybus l.)
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/b20a93c9ee694c4eb4c4affbfe318ba6
work_keys_str_mv	AT olsaybel assessmentofantioxidantactivityofwitloofchicoryherbcichoriumintybusl AT tddargaeva assessmentofantioxidantactivityofwitloofchicoryherbcichoriumintybusl AT kapupykina assessmentofantioxidantactivityofwitloofchicoryherbcichoriumintybusl AT ivpetrova assessmentofantioxidantactivityofwitloofchicoryherbcichoriumintybusl AT rrfarkhutdinov assessmentofantioxidantactivityofwitloofchicoryherbcichoriumintybusl
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ASSESSMENT OF ANTIOXIDANT ACTIVITY OF WITLOOF CHICORY HERB (CICHORIUM INTYBUS L.)

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