Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
Abstract Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial...
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Nature Portfolio
2021
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oai:doaj.org-article:b20d6767af774235b79c8b38f1f283c12021-12-02T14:58:48ZAmine modification of calcium phosphate by low-pressure plasma for bone regeneration10.1038/s41598-021-97460-82045-2322https://doaj.org/article/b20d6767af774235b79c8b38f1f283c12021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97460-8https://doaj.org/toc/2045-2322Abstract Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH4/N2/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone.Joe KodamaAnjar Anggraini HarumningtyasTomoko ItoMiroslav MichlíčekSatoshi SugimotoHidekazu KitaRyota ChijimatsuYuichiro UkonJunichi KushiokaRintaro OkadaTakashi KamataniKunihiko HashimotoDaisuke TateiwaHiroyuki TsukazakiShinichi NakagawaShota TakenakaTakahiro MakinoYusuke SakaiDavid NečasLenka ZajíčkováSatoshi HamaguchiTakashi KaitoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
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Medicine R Science Q Joe Kodama Anjar Anggraini Harumningtyas Tomoko Ito Miroslav Michlíček Satoshi Sugimoto Hidekazu Kita Ryota Chijimatsu Yuichiro Ukon Junichi Kushioka Rintaro Okada Takashi Kamatani Kunihiko Hashimoto Daisuke Tateiwa Hiroyuki Tsukazaki Shinichi Nakagawa Shota Takenaka Takahiro Makino Yusuke Sakai David Nečas Lenka Zajíčková Satoshi Hamaguchi Takashi Kaito Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
| description |
Abstract Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH4/N2/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone. |
| format |
article |
| author |
Joe Kodama Anjar Anggraini Harumningtyas Tomoko Ito Miroslav Michlíček Satoshi Sugimoto Hidekazu Kita Ryota Chijimatsu Yuichiro Ukon Junichi Kushioka Rintaro Okada Takashi Kamatani Kunihiko Hashimoto Daisuke Tateiwa Hiroyuki Tsukazaki Shinichi Nakagawa Shota Takenaka Takahiro Makino Yusuke Sakai David Nečas Lenka Zajíčková Satoshi Hamaguchi Takashi Kaito |
| author_facet |
Joe Kodama Anjar Anggraini Harumningtyas Tomoko Ito Miroslav Michlíček Satoshi Sugimoto Hidekazu Kita Ryota Chijimatsu Yuichiro Ukon Junichi Kushioka Rintaro Okada Takashi Kamatani Kunihiko Hashimoto Daisuke Tateiwa Hiroyuki Tsukazaki Shinichi Nakagawa Shota Takenaka Takahiro Makino Yusuke Sakai David Nečas Lenka Zajíčková Satoshi Hamaguchi Takashi Kaito |
| author_sort |
Joe Kodama |
| title |
Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
| title_short |
Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
| title_full |
Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
| title_fullStr |
Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
| title_full_unstemmed |
Amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
| title_sort |
amine modification of calcium phosphate by low-pressure plasma for bone regeneration |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/b20d6767af774235b79c8b38f1f283c1 |
| work_keys_str_mv |
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1718389284864524288 |