Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.

A simple susceptibility test using 800 isolates of one Campylobacter strain with different degrees of susceptibility and four bacteriophages of the British phage typing scheme was developed and examined for its suitability. The test presented is economically cheaper and less time consuming than the...

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Autores principales:	Samuel Fischer, Sophie Kittler, Günter Klein, Gerhard Glünder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b2105180f55348618d89234a18b5329d
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spelling	oai:doaj.org-article:b2105180f55348618d89234a18b5329d2021-11-18T08:01:05ZMicroplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.1932-620310.1371/journal.pone.0053899https://doaj.org/article/b2105180f55348618d89234a18b5329d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23349761/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A simple susceptibility test using 800 isolates of one Campylobacter strain with different degrees of susceptibility and four bacteriophages of the British phage typing scheme was developed and examined for its suitability. The test presented is economically cheaper and less time consuming than the conventional agar overlay plate assay and therefore enables the monitoring of changes in the susceptibility pattern during phage therapy under practical field conditions. The main objective of this study was to compare the simplified test with the conventional agar overlay plate assay. The conventional test describes for a population of Campylobacter: i. the rate of resistant isolates (0 plaques) and ii. the degree of susceptibility, also called relative efficiency of plating (EOP), for the remaining isolates. The simplified test divides the isolates into four susceptibility ranks, which are easily distinguishable to the naked eye. Ten Campylobacter isolates out of each rank were subjected to the conventional method for validation of the simplified test. Each resistance rank contained isolates showing certain degrees of susceptibility, reflecting decreasing susceptibility by an increase of the rank. Thus, the simplified test correlated well with the conventional method. Nevertheless, it can be suggested for a clear cut to summarise the first thee ranks as "high susceptible" and to mark out the fourth rank as reduced susceptible. Further test improvements will enable the monitoring of the degree of susceptibility and potentially also of resistance during phage therapy in the field. To ensure a long-lasting successful use of phage therapy, further studies on both the loss of susceptibility and the development of resistance of Campylobacter against phages combined with their impact on phage therapy will be necessary.Samuel FischerSophie KittlerGünter KleinGerhard GlünderPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53899 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samuel Fischer Sophie Kittler Günter Klein Gerhard Glünder Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.
description	A simple susceptibility test using 800 isolates of one Campylobacter strain with different degrees of susceptibility and four bacteriophages of the British phage typing scheme was developed and examined for its suitability. The test presented is economically cheaper and less time consuming than the conventional agar overlay plate assay and therefore enables the monitoring of changes in the susceptibility pattern during phage therapy under practical field conditions. The main objective of this study was to compare the simplified test with the conventional agar overlay plate assay. The conventional test describes for a population of Campylobacter: i. the rate of resistant isolates (0 plaques) and ii. the degree of susceptibility, also called relative efficiency of plating (EOP), for the remaining isolates. The simplified test divides the isolates into four susceptibility ranks, which are easily distinguishable to the naked eye. Ten Campylobacter isolates out of each rank were subjected to the conventional method for validation of the simplified test. Each resistance rank contained isolates showing certain degrees of susceptibility, reflecting decreasing susceptibility by an increase of the rank. Thus, the simplified test correlated well with the conventional method. Nevertheless, it can be suggested for a clear cut to summarise the first thee ranks as "high susceptible" and to mark out the fourth rank as reduced susceptible. Further test improvements will enable the monitoring of the degree of susceptibility and potentially also of resistance during phage therapy in the field. To ensure a long-lasting successful use of phage therapy, further studies on both the loss of susceptibility and the development of resistance of Campylobacter against phages combined with their impact on phage therapy will be necessary.
format	article
author	Samuel Fischer Sophie Kittler Günter Klein Gerhard Glünder
author_facet	Samuel Fischer Sophie Kittler Günter Klein Gerhard Glünder
author_sort	Samuel Fischer
title	Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.
title_short	Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.
title_full	Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.
title_fullStr	Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.
title_full_unstemmed	Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.
title_sort	microplate-test for the rapid determination of bacteriophage-susceptibility of campylobacter isolates-development and validation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b2105180f55348618d89234a18b5329d
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Microplate-test for the rapid determination of bacteriophage-susceptibility of Campylobacter isolates-development and validation.

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