An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

In vitro culture has detrimental effects on transcriptomes and epigenetic programming of zygotes. Here the authors use microfluidic technology to co-culture bovine oviduct epithelial cells with zygotes and show that the transcriptomes and global methylation patterns of these zygotes are more similar...

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Autores principales:	Marcia A. M. M. Ferraz, Hoon Suk Rho, Daiane Hemerich, Heiko H. W. Henning, Helena T. A. van Tol, Michael Hölker, Urban Besenfelder, Michal Mokry, Peter L. A. M. Vos, Tom A. E. Stout, Séverine Le Gac, Bart M. Gadella
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b2189c0b6d4a43509e715b245fc4d76a
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spelling	oai:doaj.org-article:b2189c0b6d4a43509e715b245fc4d76a2021-12-02T14:41:17ZAn oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming10.1038/s41467-018-07119-82041-1723https://doaj.org/article/b2189c0b6d4a43509e715b245fc4d76a2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07119-8https://doaj.org/toc/2041-1723In vitro culture has detrimental effects on transcriptomes and epigenetic programming of zygotes. Here the authors use microfluidic technology to co-culture bovine oviduct epithelial cells with zygotes and show that the transcriptomes and global methylation patterns of these zygotes are more similar to in vivo zygotes than to conventionally cultured zygotes.Marcia A. M. M. FerrazHoon Suk RhoDaiane HemerichHeiko H. W. HenningHelena T. A. van TolMichael HölkerUrban BesenfelderMichal MokryPeter L. A. M. VosTom A. E. StoutSéverine Le GacBart M. GadellaNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Marcia A. M. M. Ferraz Hoon Suk Rho Daiane Hemerich Heiko H. W. Henning Helena T. A. van Tol Michael Hölker Urban Besenfelder Michal Mokry Peter L. A. M. Vos Tom A. E. Stout Séverine Le Gac Bart M. Gadella An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
description	In vitro culture has detrimental effects on transcriptomes and epigenetic programming of zygotes. Here the authors use microfluidic technology to co-culture bovine oviduct epithelial cells with zygotes and show that the transcriptomes and global methylation patterns of these zygotes are more similar to in vivo zygotes than to conventionally cultured zygotes.
format	article
author	Marcia A. M. M. Ferraz Hoon Suk Rho Daiane Hemerich Heiko H. W. Henning Helena T. A. van Tol Michael Hölker Urban Besenfelder Michal Mokry Peter L. A. M. Vos Tom A. E. Stout Séverine Le Gac Bart M. Gadella
author_facet	Marcia A. M. M. Ferraz Hoon Suk Rho Daiane Hemerich Heiko H. W. Henning Helena T. A. van Tol Michael Hölker Urban Besenfelder Michal Mokry Peter L. A. M. Vos Tom A. E. Stout Séverine Le Gac Bart M. Gadella
author_sort	Marcia A. M. M. Ferraz
title	An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
title_short	An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
title_full	An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
title_fullStr	An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
title_full_unstemmed	An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
title_sort	oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b2189c0b6d4a43509e715b245fc4d76a
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An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

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