Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing

Abstract We xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they...

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Autores principales:	Giulia Nato, Alessandro Corti, Elena Parmigiani, Elena Jachetti, Daniele Lecis, Mario Paolo Colombo, Domenico Delia, Annalisa Buffo, Lorenzo Magrassi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b218f6be9a034a4a967efb0818500804
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spelling	oai:doaj.org-article:b218f6be9a034a4a967efb08185008042021-12-02T14:12:07ZImmune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing10.1038/s41598-020-79502-92045-2322https://doaj.org/article/b218f6be9a034a4a967efb08185008042021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79502-9https://doaj.org/toc/2045-2322Abstract We xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they were rejected by both species. Extended survival and differentiation of the same cells were obtained only when they were transplanted in NOD-SCID mice. Transplants of human neural precursor cells mixed with the same cells after partial in vitro differentiation or with a cellular extract obtained from adult rat cerebellum increased survival of the xeno-graft beyond one month. These findings are consistent with the hypothesis that the slower pace of differentiation of human neural precursors compared to that of rodents restricts induction of immune-tolerance to human antigens expressed before completion of maturation of the immune system. With further maturation the transplanted neural precursors expressed more mature antigens before the graft were rejected. Supplementation of the immature cells suspensions with more mature antigens may help to induce immune-tolerance for those antigens expressed only later by the engrafted cells.Giulia NatoAlessandro CortiElena ParmigianiElena JachettiDaniele LecisMario Paolo ColomboDomenico DeliaAnnalisa BuffoLorenzo MagrassiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giulia Nato Alessandro Corti Elena Parmigiani Elena Jachetti Daniele Lecis Mario Paolo Colombo Domenico Delia Annalisa Buffo Lorenzo Magrassi Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
description	Abstract We xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they were rejected by both species. Extended survival and differentiation of the same cells were obtained only when they were transplanted in NOD-SCID mice. Transplants of human neural precursor cells mixed with the same cells after partial in vitro differentiation or with a cellular extract obtained from adult rat cerebellum increased survival of the xeno-graft beyond one month. These findings are consistent with the hypothesis that the slower pace of differentiation of human neural precursors compared to that of rodents restricts induction of immune-tolerance to human antigens expressed before completion of maturation of the immune system. With further maturation the transplanted neural precursors expressed more mature antigens before the graft were rejected. Supplementation of the immature cells suspensions with more mature antigens may help to induce immune-tolerance for those antigens expressed only later by the engrafted cells.
format	article
author	Giulia Nato Alessandro Corti Elena Parmigiani Elena Jachetti Daniele Lecis Mario Paolo Colombo Domenico Delia Annalisa Buffo Lorenzo Magrassi
author_facet	Giulia Nato Alessandro Corti Elena Parmigiani Elena Jachetti Daniele Lecis Mario Paolo Colombo Domenico Delia Annalisa Buffo Lorenzo Magrassi
author_sort	Giulia Nato
title	Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
title_short	Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
title_full	Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
title_fullStr	Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
title_full_unstemmed	Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
title_sort	immune-tolerance to human ips-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b218f6be9a034a4a967efb0818500804
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Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing

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