Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging

Yunn-Hwa Ma,1 Si-Yi Chen,1 Shu-Ju Tu,2 Hung-Wei Yang,3 Hao-Li Liu41Department of Physiology and Pharmacology and Healthy Aging Research Center, 2Department of Medical Image and Radiological Sciences, 3Department of Chemical and Material Engineering, 4Department of Electrical Engineering, College of...

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Autores principales:	Ma YH, Chen SY, Tu SJ, Yang HW, Liu HL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b2232598bdab43df8f69a6e64c9b00fd
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spelling	oai:doaj.org-article:b2232598bdab43df8f69a6e64c9b00fd2021-12-02T00:58:52ZManipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging1176-91141178-2013https://doaj.org/article/b2232598bdab43df8f69a6e64c9b00fd2012-06-01T00:00:00Zhttp://www.dovepress.com/manipulation-of-magnetic-nanoparticle-retention-and-hemodynamic-conseq-a10074https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yunn-Hwa Ma,1 Si-Yi Chen,1 Shu-Ju Tu,2 Hung-Wei Yang,3 Hao-Li Liu41Department of Physiology and Pharmacology and Healthy Aging Research Center, 2Department of Medical Image and Radiological Sciences, 3Department of Chemical and Material Engineering, 4Department of Electrical Engineering, College of Medicine and College of Engineering, Chang Gung University, Kuei-Shan, Tao-Yuan, Taiwan, ROCAbstract: Magnetic nanoparticles (MNPs) have been proposed for targeted or embolization therapeutics. How MNP retention occurs in circulation may critically determine local hemodynamics, tissue distribution of MNPs, and the therapeutic effects. We attempted to establish a microcirculation model to study the magnetic capture of MNPs in small vessels and to determine the factors affecting MNP retention. Two-dimensional hemodynamic changes in response to magnet-induced MNP retention in the microvessels of the cremaster muscle in vivo were observed in a real-time manner using a laser speckle imaging technique. Changes in tissue perfusion of the cremaster muscle appeared to be closely correlated with the location of the magnet placement underneath the muscle in response to intra-arterial administration of dextran-coated MNPs. Magnet-related retention was observed along the edge of the magnet, as corroborated by the results of histology analysis and microcomputed tomography. In these preparations, tissue iron content almost doubled, as revealed by inductively coupled plasma optical emission spectroscopy. In addition, MNP retention was associated with reduced downstream flow in a dose-dependent manner. Dissipation of MNPs (5 mg/kg) occurred shortly after removal of the magnet, which was associated with significant recovery of tissue flow. However, MNP dissipation did not easily occur after administration of a higher MNP dose (10 mg/kg) or prolonged exposure to the magnetic field. An ultrasound after removal of the magnet may induce the partial dispersion of MNPs and thus partially improve hemodynamics. In conclusion, our results revealed the important correlation of local MNP retention and hemodynamic changes in microcirculation, which can be crucial in the application of MNPs for effective targeted therapeutics.Keywords: targeted delivery, magnetic nanoparticles, hemodynamics, microcirculationMa YHChen SYTu SJYang HWLiu HLDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2817-2827 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ma YH Chen SY Tu SJ Yang HW Liu HL Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging
description	Yunn-Hwa Ma,1 Si-Yi Chen,1 Shu-Ju Tu,2 Hung-Wei Yang,3 Hao-Li Liu41Department of Physiology and Pharmacology and Healthy Aging Research Center, 2Department of Medical Image and Radiological Sciences, 3Department of Chemical and Material Engineering, 4Department of Electrical Engineering, College of Medicine and College of Engineering, Chang Gung University, Kuei-Shan, Tao-Yuan, Taiwan, ROCAbstract: Magnetic nanoparticles (MNPs) have been proposed for targeted or embolization therapeutics. How MNP retention occurs in circulation may critically determine local hemodynamics, tissue distribution of MNPs, and the therapeutic effects. We attempted to establish a microcirculation model to study the magnetic capture of MNPs in small vessels and to determine the factors affecting MNP retention. Two-dimensional hemodynamic changes in response to magnet-induced MNP retention in the microvessels of the cremaster muscle in vivo were observed in a real-time manner using a laser speckle imaging technique. Changes in tissue perfusion of the cremaster muscle appeared to be closely correlated with the location of the magnet placement underneath the muscle in response to intra-arterial administration of dextran-coated MNPs. Magnet-related retention was observed along the edge of the magnet, as corroborated by the results of histology analysis and microcomputed tomography. In these preparations, tissue iron content almost doubled, as revealed by inductively coupled plasma optical emission spectroscopy. In addition, MNP retention was associated with reduced downstream flow in a dose-dependent manner. Dissipation of MNPs (5 mg/kg) occurred shortly after removal of the magnet, which was associated with significant recovery of tissue flow. However, MNP dissipation did not easily occur after administration of a higher MNP dose (10 mg/kg) or prolonged exposure to the magnetic field. An ultrasound after removal of the magnet may induce the partial dispersion of MNPs and thus partially improve hemodynamics. In conclusion, our results revealed the important correlation of local MNP retention and hemodynamic changes in microcirculation, which can be crucial in the application of MNPs for effective targeted therapeutics.Keywords: targeted delivery, magnetic nanoparticles, hemodynamics, microcirculation
format	article
author	Ma YH Chen SY Tu SJ Yang HW Liu HL
author_facet	Ma YH Chen SY Tu SJ Yang HW Liu HL
author_sort	Ma YH
title	Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging
title_short	Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging
title_full	Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging
title_fullStr	Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging
title_full_unstemmed	Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging
title_sort	manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/b2232598bdab43df8f69a6e64c9b00fd
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Manipulation of magnetic nanoparticle retention and hemodynamic consequences in microcirculation: assessment by laser speckle imaging

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