Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes

Acute lymphoblastic leukemia (ALL) is the most common type of malignant neoplasms in the pediatric population. B-cell precursor ALLs (BCP-ALLs) are derived from the progenitors of B lymphocytes. Traditionally, risk factors stratifying therapy in ALL patients included age at diagnosis, initial leukoc...

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Autores principales:	Patrycja Leszczenko, Aleksandra Borek-Dorosz, Anna Maria Nowakowska, Adriana Adamczyk, Sviatlana Kashyrskaya, Justyna Jakubowska, Marta Ząbczyńska, Agata Pastorczak, Kinga Ostrowska, Malgorzata Baranska, Katarzyna Maria Marzec, Katarzyna Majzner
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Raman spectroscopy acute lymphoblastic leukemia (ALL) fusion genes chemometric techniques <i>BCR-ABL1</i> <i>TCF-PBX1</i> Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/b23478871ab340618ebc3930ee9d4788
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spelling	oai:doaj.org-article:b23478871ab340618ebc3930ee9d47882021-11-11T15:33:40ZTowards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes10.3390/cancers132154832072-6694https://doaj.org/article/b23478871ab340618ebc3930ee9d47882021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5483https://doaj.org/toc/2072-6694Acute lymphoblastic leukemia (ALL) is the most common type of malignant neoplasms in the pediatric population. B-cell precursor ALLs (BCP-ALLs) are derived from the progenitors of B lymphocytes. Traditionally, risk factors stratifying therapy in ALL patients included age at diagnosis, initial leukocytosis, and the response to chemotherapy. Currently, treatment intensity is modified according to the presence of specific gene alterations in the leukemic genome. Raman imaging is a promising diagnostic tool, which enables the molecular characterization of cells and differentiation of subtypes of leukemia in clinical samples. This study aimed to characterize and distinguish cells isolated from the bone marrow of patients suffering from three subtypes of BCP-ALL, defined by gene rearrangements, i.e., <i>BCR-ABL1</i> <i>(Philadelphia</i>-positive, t(9;22)), <i>TEL-AML1</i> (t(12;21)) and <i>TCF3-PBX1</i> (t(1;19)), using single-cell Raman imaging combined with multivariate statistical analysis. Spectra collected from clinical samples were compared with single-cell spectra of B-cells collected from healthy donors, constituting the control group. We demonstrated that Raman spectra of normal B cells strongly differ from spectra of their malignant counterparts, especially in the intensity of bands, which can be assigned to nucleic acids. We also showed that the identification of leukemia subtypes could be automated with the use of chemometric methods. Results prove the clinical suitability of Raman imaging for the identification of spectroscopic markers characterizing leukemia cells.Patrycja LeszczenkoAleksandra Borek-DoroszAnna Maria NowakowskaAdriana AdamczykSviatlana KashyrskayaJustyna JakubowskaMarta ZąbczyńskaAgata PastorczakKinga OstrowskaMalgorzata BaranskaKatarzyna Maria MarzecKatarzyna MajznerMDPI AGarticleRaman spectroscopyacute lymphoblastic leukemia (ALL)fusion geneschemometric techniques<i>BCR-ABL1</i><i>TCF-PBX1</i>Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5483, p 5483 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Raman spectroscopy acute lymphoblastic leukemia (ALL) fusion genes chemometric techniques <i>BCR-ABL1</i> <i>TCF-PBX1</i> Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Raman spectroscopy acute lymphoblastic leukemia (ALL) fusion genes chemometric techniques <i>BCR-ABL1</i> <i>TCF-PBX1</i> Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Patrycja Leszczenko Aleksandra Borek-Dorosz Anna Maria Nowakowska Adriana Adamczyk Sviatlana Kashyrskaya Justyna Jakubowska Marta Ząbczyńska Agata Pastorczak Kinga Ostrowska Malgorzata Baranska Katarzyna Maria Marzec Katarzyna Majzner Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes
description	Acute lymphoblastic leukemia (ALL) is the most common type of malignant neoplasms in the pediatric population. B-cell precursor ALLs (BCP-ALLs) are derived from the progenitors of B lymphocytes. Traditionally, risk factors stratifying therapy in ALL patients included age at diagnosis, initial leukocytosis, and the response to chemotherapy. Currently, treatment intensity is modified according to the presence of specific gene alterations in the leukemic genome. Raman imaging is a promising diagnostic tool, which enables the molecular characterization of cells and differentiation of subtypes of leukemia in clinical samples. This study aimed to characterize and distinguish cells isolated from the bone marrow of patients suffering from three subtypes of BCP-ALL, defined by gene rearrangements, i.e., <i>BCR-ABL1</i> <i>(Philadelphia</i>-positive, t(9;22)), <i>TEL-AML1</i> (t(12;21)) and <i>TCF3-PBX1</i> (t(1;19)), using single-cell Raman imaging combined with multivariate statistical analysis. Spectra collected from clinical samples were compared with single-cell spectra of B-cells collected from healthy donors, constituting the control group. We demonstrated that Raman spectra of normal B cells strongly differ from spectra of their malignant counterparts, especially in the intensity of bands, which can be assigned to nucleic acids. We also showed that the identification of leukemia subtypes could be automated with the use of chemometric methods. Results prove the clinical suitability of Raman imaging for the identification of spectroscopic markers characterizing leukemia cells.
format	article
author	Patrycja Leszczenko Aleksandra Borek-Dorosz Anna Maria Nowakowska Adriana Adamczyk Sviatlana Kashyrskaya Justyna Jakubowska Marta Ząbczyńska Agata Pastorczak Kinga Ostrowska Malgorzata Baranska Katarzyna Maria Marzec Katarzyna Majzner
author_facet	Patrycja Leszczenko Aleksandra Borek-Dorosz Anna Maria Nowakowska Adriana Adamczyk Sviatlana Kashyrskaya Justyna Jakubowska Marta Ząbczyńska Agata Pastorczak Kinga Ostrowska Malgorzata Baranska Katarzyna Maria Marzec Katarzyna Majzner
author_sort	Patrycja Leszczenko
title	Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes
title_short	Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes
title_full	Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes
title_fullStr	Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes
title_full_unstemmed	Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes
title_sort	towards raman-based screening of acute lymphoblastic leukemia-type b (b-all) subtypes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b23478871ab340618ebc3930ee9d4788
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Towards Raman-Based Screening of Acute Lymphoblastic Leukemia-Type B (B-ALL) Subtypes

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