Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs

Abstract Multiresistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) cause serious postoperative infections. A skin tolerant far-UVC (< 240 nm) irradiation system for their inactivation is presented here. It uses UVC LEDs in combination with a spectral filter and provides...

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Autores principales:	Johannes Glaab, Neysha Lobo-Ploch, Hyun Kyong Cho, Thomas Filler, Heiko Gundlach, Martin Guttmann, Sylvia Hagedorn, Silke B. Lohan, Frank Mehnke, Johannes Schleusener, Claudia Sicher, Luca Sulmoni, Tim Wernicke, Lucas Wittenbecher, Ulrike Woggon, Paula Zwicker, Axel Kramer, Martina C. Meinke, Michael Kneissl, Markus Weyers, Ulrike Winterwerber, Sven Einfeldt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b236578f9a8f4b3189007bc71395130c
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spelling	oai:doaj.org-article:b236578f9a8f4b3189007bc71395130c2021-12-02T17:03:50ZSkin tolerant inactivation of multiresistant pathogens using far-UVC LEDs10.1038/s41598-021-94070-22045-2322https://doaj.org/article/b236578f9a8f4b3189007bc71395130c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94070-2https://doaj.org/toc/2045-2322Abstract Multiresistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) cause serious postoperative infections. A skin tolerant far-UVC (< 240 nm) irradiation system for their inactivation is presented here. It uses UVC LEDs in combination with a spectral filter and provides a peak wavelength of 233 nm, with a full width at half maximum of 12 nm, and an irradiance of 44 µW/cm2. MRSA bacteria in different concentrations on blood agar plates were inactivated with irradiation doses in the range of 15–40 mJ/cm2. Porcine skin irradiated with a dose of 40 mJ/cm2 at 233 nm showed only 3.7% CPD and 2.3% 6-4PP DNA damage. Corresponding irradiation at 254 nm caused 15–30 times higher damage. Thus, the skin damage caused by the disinfectant doses is so small that it can be expected to be compensated by the skin's natural repair mechanisms. LED-based far-UVC lamps could therefore soon be used in everyday clinical practice to eradicate multiresistant pathogens directly on humans.Johannes GlaabNeysha Lobo-PlochHyun Kyong ChoThomas FillerHeiko GundlachMartin GuttmannSylvia HagedornSilke B. LohanFrank MehnkeJohannes SchleusenerClaudia SicherLuca SulmoniTim WernickeLucas WittenbecherUlrike WoggonPaula ZwickerAxel KramerMartina C. MeinkeMichael KneisslMarkus WeyersUlrike WinterwerberSven EinfeldtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johannes Glaab Neysha Lobo-Ploch Hyun Kyong Cho Thomas Filler Heiko Gundlach Martin Guttmann Sylvia Hagedorn Silke B. Lohan Frank Mehnke Johannes Schleusener Claudia Sicher Luca Sulmoni Tim Wernicke Lucas Wittenbecher Ulrike Woggon Paula Zwicker Axel Kramer Martina C. Meinke Michael Kneissl Markus Weyers Ulrike Winterwerber Sven Einfeldt Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs
description	Abstract Multiresistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) cause serious postoperative infections. A skin tolerant far-UVC (< 240 nm) irradiation system for their inactivation is presented here. It uses UVC LEDs in combination with a spectral filter and provides a peak wavelength of 233 nm, with a full width at half maximum of 12 nm, and an irradiance of 44 µW/cm2. MRSA bacteria in different concentrations on blood agar plates were inactivated with irradiation doses in the range of 15–40 mJ/cm2. Porcine skin irradiated with a dose of 40 mJ/cm2 at 233 nm showed only 3.7% CPD and 2.3% 6-4PP DNA damage. Corresponding irradiation at 254 nm caused 15–30 times higher damage. Thus, the skin damage caused by the disinfectant doses is so small that it can be expected to be compensated by the skin's natural repair mechanisms. LED-based far-UVC lamps could therefore soon be used in everyday clinical practice to eradicate multiresistant pathogens directly on humans.
format	article
author	Johannes Glaab Neysha Lobo-Ploch Hyun Kyong Cho Thomas Filler Heiko Gundlach Martin Guttmann Sylvia Hagedorn Silke B. Lohan Frank Mehnke Johannes Schleusener Claudia Sicher Luca Sulmoni Tim Wernicke Lucas Wittenbecher Ulrike Woggon Paula Zwicker Axel Kramer Martina C. Meinke Michael Kneissl Markus Weyers Ulrike Winterwerber Sven Einfeldt
author_facet	Johannes Glaab Neysha Lobo-Ploch Hyun Kyong Cho Thomas Filler Heiko Gundlach Martin Guttmann Sylvia Hagedorn Silke B. Lohan Frank Mehnke Johannes Schleusener Claudia Sicher Luca Sulmoni Tim Wernicke Lucas Wittenbecher Ulrike Woggon Paula Zwicker Axel Kramer Martina C. Meinke Michael Kneissl Markus Weyers Ulrike Winterwerber Sven Einfeldt
author_sort	Johannes Glaab
title	Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs
title_short	Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs
title_full	Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs
title_fullStr	Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs
title_full_unstemmed	Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs
title_sort	skin tolerant inactivation of multiresistant pathogens using far-uvc leds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b236578f9a8f4b3189007bc71395130c
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Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs

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