Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus

Abstract Background Type II diabetes mellitus (T2DM) is an important risk factor for osseointegration of implants. The aim of this study was to explore key genes of T2DM affecting bone metabolism through bioinformatic analysis of published RNA sequencing data, identify potential biomarkers, and prov...

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Autores principales:	Lingxiao Wang, Zhenhua Gao, Changying Liu, Jun Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bioinformatics analysis Biomarkers Osseointegration miRNAs T2DM Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/b2367f66993042d2987dcb0514ec8443
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spelling	oai:doaj.org-article:b2367f66993042d2987dcb0514ec84432021-11-21T12:32:25ZPotential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus10.1186/s12903-021-01939-91472-6831https://doaj.org/article/b2367f66993042d2987dcb0514ec84432021-11-01T00:00:00Zhttps://doi.org/10.1186/s12903-021-01939-9https://doaj.org/toc/1472-6831Abstract Background Type II diabetes mellitus (T2DM) is an important risk factor for osseointegration of implants. The aim of this study was to explore key genes of T2DM affecting bone metabolism through bioinformatic analysis of published RNA sequencing data, identify potential biomarkers, and provide a reference for finding the molecular mechanism of abnormal osseointegration caused by T2DM. Methods We identified differentially expressed mRNAs and miRNAs from the Gene Expression Omnibus database using the R package ‘limma’ and analysed the predicted target genes using Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene Ontology analysis. At the same time, miRNA–mRNA interactions were explored using miRWalk 2.0. Results We constructed an miRNA-gene regulatory network and a protein–protein interaction network. The enrichment pathways of differentially expressed mRNAs included extracellular matrix receptor interactions, protein digestion and absorption, the PI3K-Akt signalling pathway, cytokine–cytokine receptor interactions, chemokine signalling pathways, and haematopoietic cell lineage functions. We analysed the expression of these differentially expressed mRNAs and miRNAs in T2DM rats and normal rats with bone implants and identified Smpd3, Itga10, and rno-mir-207 as possible key players in osseointegration in T2DM. Conclusion Smpd3, Itga10, and rno-mir-207 are possible biomarkers of osseointegration in T2DM. This study sheds light on the possible molecular mechanism of abnormal osseointegration caused by bone metabolism disorder in T2DM.Lingxiao WangZhenhua GaoChangying LiuJun LiBMCarticleBioinformatics analysisBiomarkersOsseointegrationmiRNAsT2DMDentistryRK1-715ENBMC Oral Health, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bioinformatics analysis Biomarkers Osseointegration miRNAs T2DM Dentistry RK1-715
spellingShingle	Bioinformatics analysis Biomarkers Osseointegration miRNAs T2DM Dentistry RK1-715 Lingxiao Wang Zhenhua Gao Changying Liu Jun Li Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus
description	Abstract Background Type II diabetes mellitus (T2DM) is an important risk factor for osseointegration of implants. The aim of this study was to explore key genes of T2DM affecting bone metabolism through bioinformatic analysis of published RNA sequencing data, identify potential biomarkers, and provide a reference for finding the molecular mechanism of abnormal osseointegration caused by T2DM. Methods We identified differentially expressed mRNAs and miRNAs from the Gene Expression Omnibus database using the R package ‘limma’ and analysed the predicted target genes using Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene Ontology analysis. At the same time, miRNA–mRNA interactions were explored using miRWalk 2.0. Results We constructed an miRNA-gene regulatory network and a protein–protein interaction network. The enrichment pathways of differentially expressed mRNAs included extracellular matrix receptor interactions, protein digestion and absorption, the PI3K-Akt signalling pathway, cytokine–cytokine receptor interactions, chemokine signalling pathways, and haematopoietic cell lineage functions. We analysed the expression of these differentially expressed mRNAs and miRNAs in T2DM rats and normal rats with bone implants and identified Smpd3, Itga10, and rno-mir-207 as possible key players in osseointegration in T2DM. Conclusion Smpd3, Itga10, and rno-mir-207 are possible biomarkers of osseointegration in T2DM. This study sheds light on the possible molecular mechanism of abnormal osseointegration caused by bone metabolism disorder in T2DM.
format	article
author	Lingxiao Wang Zhenhua Gao Changying Liu Jun Li
author_facet	Lingxiao Wang Zhenhua Gao Changying Liu Jun Li
author_sort	Lingxiao Wang
title	Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus
title_short	Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus
title_full	Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus
title_fullStr	Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus
title_full_unstemmed	Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus
title_sort	potential biomarkers of abnormal osseointegration of implants in type ii diabetes mellitus
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b2367f66993042d2987dcb0514ec8443
work_keys_str_mv	AT lingxiaowang potentialbiomarkersofabnormalosseointegrationofimplantsintypeiidiabetesmellitus AT zhenhuagao potentialbiomarkersofabnormalosseointegrationofimplantsintypeiidiabetesmellitus AT changyingliu potentialbiomarkersofabnormalosseointegrationofimplantsintypeiidiabetesmellitus AT junli potentialbiomarkersofabnormalosseointegrationofimplantsintypeiidiabetesmellitus
_version_	1718418965057765376

Potential biomarkers of abnormal osseointegration of implants in type II diabetes mellitus

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