Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice

Abstract Although Zika virus (ZIKV) is primarily transmitted to humans by the Aedes aegypti mosquito, human-to-human transmission has also been observed from males-to-females as well as mother-to-offspring. In the current study, we studied both sexual transmission (STx) and vertical transmission (VT...

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Autores principales:	Clayton W. Winkler, Tyson A. Woods, Rebecca Rosenke, Dana P. Scott, Sonja M. Best, Karin E. Peterson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b2388bceb32b45cc95f762289037d0c8
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spelling	oai:doaj.org-article:b2388bceb32b45cc95f762289037d0c82021-12-02T12:31:59ZSexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice10.1038/s41598-017-07099-72045-2322https://doaj.org/article/b2388bceb32b45cc95f762289037d0c82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07099-7https://doaj.org/toc/2045-2322Abstract Although Zika virus (ZIKV) is primarily transmitted to humans by the Aedes aegypti mosquito, human-to-human transmission has also been observed from males-to-females as well as mother-to-offspring. In the current study, we studied both sexual transmission (STx) and vertical transmission (VTx) of ZIKV using anti-IFNAR1-treatment of Rag1 −/− (AIR) mice. These mice have suppressed type I IFN responses and lack adaptive immune responses, leading to a prolonged infection prior to clinical disease. STx of ZIKV from infected AIR males to naive Ifnar1 −/− females was observed with greater than 50% incidence, with infection observed in the vaginal tract at early time points. In the case of a resulting pregnancy, virus was also found in the uterus and placental tissue. In additional studies, VTx of virus was observed in AIR female mice. Specifically, peripheral ZIKV infection of pregnant AIR females resulted in detectable virus in brain and/or lymph nodes of fetuses and/or pups. VTx of ZIKV was stochastic, in that not all fetuses/pups within the same dam had detectable virus and infection was not associated with breakdown of maternal/fetal placental barrier. This provides a new model to study the barriers to STx and VTx of ZIKV and the immune responses essential to preventing transmission.Clayton W. WinklerTyson A. WoodsRebecca RosenkeDana P. ScottSonja M. BestKarin E. PetersonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Clayton W. Winkler Tyson A. Woods Rebecca Rosenke Dana P. Scott Sonja M. Best Karin E. Peterson Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice
description	Abstract Although Zika virus (ZIKV) is primarily transmitted to humans by the Aedes aegypti mosquito, human-to-human transmission has also been observed from males-to-females as well as mother-to-offspring. In the current study, we studied both sexual transmission (STx) and vertical transmission (VTx) of ZIKV using anti-IFNAR1-treatment of Rag1 −/− (AIR) mice. These mice have suppressed type I IFN responses and lack adaptive immune responses, leading to a prolonged infection prior to clinical disease. STx of ZIKV from infected AIR males to naive Ifnar1 −/− females was observed with greater than 50% incidence, with infection observed in the vaginal tract at early time points. In the case of a resulting pregnancy, virus was also found in the uterus and placental tissue. In additional studies, VTx of virus was observed in AIR female mice. Specifically, peripheral ZIKV infection of pregnant AIR females resulted in detectable virus in brain and/or lymph nodes of fetuses and/or pups. VTx of ZIKV was stochastic, in that not all fetuses/pups within the same dam had detectable virus and infection was not associated with breakdown of maternal/fetal placental barrier. This provides a new model to study the barriers to STx and VTx of ZIKV and the immune responses essential to preventing transmission.
format	article
author	Clayton W. Winkler Tyson A. Woods Rebecca Rosenke Dana P. Scott Sonja M. Best Karin E. Peterson
author_facet	Clayton W. Winkler Tyson A. Woods Rebecca Rosenke Dana P. Scott Sonja M. Best Karin E. Peterson
author_sort	Clayton W. Winkler
title	Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice
title_short	Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice
title_full	Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice
title_fullStr	Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice
title_full_unstemmed	Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice
title_sort	sexual and vertical transmission of zika virus in anti-interferon receptor-treated rag1-deficient mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b2388bceb32b45cc95f762289037d0c8
work_keys_str_mv	AT claytonwwinkler sexualandverticaltransmissionofzikavirusinantiinterferonreceptortreatedrag1deficientmice AT tysonawoods sexualandverticaltransmissionofzikavirusinantiinterferonreceptortreatedrag1deficientmice AT rebeccarosenke sexualandverticaltransmissionofzikavirusinantiinterferonreceptortreatedrag1deficientmice AT danapscott sexualandverticaltransmissionofzikavirusinantiinterferonreceptortreatedrag1deficientmice AT sonjambest sexualandverticaltransmissionofzikavirusinantiinterferonreceptortreatedrag1deficientmice AT karinepeterson sexualandverticaltransmissionofzikavirusinantiinterferonreceptortreatedrag1deficientmice
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Sexual and Vertical Transmission of Zika Virus in anti-interferon receptor-treated Rag1-deficient mice

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