Pre- and postmenopausal women have different core urinary microbiota

Abstract Recent studies suggest that alterations in the female urinary microbiota is associated to development of bladder disease. However, the normal microbiota composition and variation in healthy women are poorly described. Moreover, the effects of hormonal changes on microbiota during menopause...

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Autores principales:	Nadia Ammitzbøll, Benedikt Paul Josef Bau, Caspar Bundgaard-Nielsen, Annemarie Brusen Villadsen, Ann-Maria Jensen, Peter Derek Christian Leutscher, Karin Glavind, Søren Hagstrøm, Louise Thomsen Schmidt Arenholt, Suzette Sørensen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b2434008c9724b0a9f5ba5a2204c48df
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spelling	oai:doaj.org-article:b2434008c9724b0a9f5ba5a2204c48df2021-12-02T10:48:22ZPre- and postmenopausal women have different core urinary microbiota10.1038/s41598-021-81790-82045-2322https://doaj.org/article/b2434008c9724b0a9f5ba5a2204c48df2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81790-8https://doaj.org/toc/2045-2322Abstract Recent studies suggest that alterations in the female urinary microbiota is associated to development of bladder disease. However, the normal microbiota composition and variation in healthy women are poorly described. Moreover, the effects of hormonal changes on microbiota during menopause is not well understood. The aim of our study was to investigate the urinary microbiota in healthy pre- and postmenopausal women without urinary tract symptoms. Microbiota composition in catheterized urine samples was mapped using 16S rRNA gene sequencing. In total, 41 premenopausal and 42 postmenopausal women were initially included. Samples with first PCR amplification concentration below level of the negative control were excluded, resulting in 34 premenopausal and 20 postmenopausal women included in data analysis. Urine from postmenopausal women showed significantly higher alpha diversity compared to premenopausal women. Lactobacillus was the most abundant bacteria in both groups, however the relative abundance of Lactobacillus accounted for 77.8% in premenopausal versus 42.0% in postmenopausal women. In conclusion, urine from premenopausal mostly presented with Lactobacillus dominated urotypes, whereas urine from postmenopausal women presented a more diverse urinary microbiota with higher abundance of the genera Gardnerella and Prevotella. The clinical and pathophysiological implications of this difference remain to be elucidated.Nadia AmmitzbøllBenedikt Paul Josef BauCaspar Bundgaard-NielsenAnnemarie Brusen VilladsenAnn-Maria JensenPeter Derek Christian LeutscherKarin GlavindSøren HagstrømLouise Thomsen Schmidt ArenholtSuzette SørensenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nadia Ammitzbøll Benedikt Paul Josef Bau Caspar Bundgaard-Nielsen Annemarie Brusen Villadsen Ann-Maria Jensen Peter Derek Christian Leutscher Karin Glavind Søren Hagstrøm Louise Thomsen Schmidt Arenholt Suzette Sørensen Pre- and postmenopausal women have different core urinary microbiota
description	Abstract Recent studies suggest that alterations in the female urinary microbiota is associated to development of bladder disease. However, the normal microbiota composition and variation in healthy women are poorly described. Moreover, the effects of hormonal changes on microbiota during menopause is not well understood. The aim of our study was to investigate the urinary microbiota in healthy pre- and postmenopausal women without urinary tract symptoms. Microbiota composition in catheterized urine samples was mapped using 16S rRNA gene sequencing. In total, 41 premenopausal and 42 postmenopausal women were initially included. Samples with first PCR amplification concentration below level of the negative control were excluded, resulting in 34 premenopausal and 20 postmenopausal women included in data analysis. Urine from postmenopausal women showed significantly higher alpha diversity compared to premenopausal women. Lactobacillus was the most abundant bacteria in both groups, however the relative abundance of Lactobacillus accounted for 77.8% in premenopausal versus 42.0% in postmenopausal women. In conclusion, urine from premenopausal mostly presented with Lactobacillus dominated urotypes, whereas urine from postmenopausal women presented a more diverse urinary microbiota with higher abundance of the genera Gardnerella and Prevotella. The clinical and pathophysiological implications of this difference remain to be elucidated.
format	article
author	Nadia Ammitzbøll Benedikt Paul Josef Bau Caspar Bundgaard-Nielsen Annemarie Brusen Villadsen Ann-Maria Jensen Peter Derek Christian Leutscher Karin Glavind Søren Hagstrøm Louise Thomsen Schmidt Arenholt Suzette Sørensen
author_facet	Nadia Ammitzbøll Benedikt Paul Josef Bau Caspar Bundgaard-Nielsen Annemarie Brusen Villadsen Ann-Maria Jensen Peter Derek Christian Leutscher Karin Glavind Søren Hagstrøm Louise Thomsen Schmidt Arenholt Suzette Sørensen
author_sort	Nadia Ammitzbøll
title	Pre- and postmenopausal women have different core urinary microbiota
title_short	Pre- and postmenopausal women have different core urinary microbiota
title_full	Pre- and postmenopausal women have different core urinary microbiota
title_fullStr	Pre- and postmenopausal women have different core urinary microbiota
title_full_unstemmed	Pre- and postmenopausal women have different core urinary microbiota
title_sort	pre- and postmenopausal women have different core urinary microbiota
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b2434008c9724b0a9f5ba5a2204c48df
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Pre- and postmenopausal women have different core urinary microbiota

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