Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)

Abstract. Mansyur, Karti PMH, Abdullah L, Husni A, Lestari P. 2019. Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR). Biodiversitas 20: 2403-2409. Napiergrass is one of the tropical grasses which has a very important role in developing ruminant li...

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Autores principales:	Mansyur M Mansyur, Panca Dewi MH Karti, Luki Abdullah, Ali Husni, Puji Lestari
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	est-ssr, forage, genetic diversity, napiergrass Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b24672135f484065b62eab7857089cbe
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spelling	oai:doaj.org-article:b24672135f484065b62eab7857089cbe2021-11-21T21:43:54ZGenetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)1412-033X2085-472210.13057/biodiv/d200839https://doaj.org/article/b24672135f484065b62eab7857089cbe2019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4071https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Mansyur, Karti PMH, Abdullah L, Husni A, Lestari P. 2019. Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR). Biodiversitas 20: 2403-2409. Napiergrass is one of the tropical grasses which has a very important role in developing ruminant livestock, its productivity is high and its nutritional content is quite good. Plant breeding to produce new varieties that have better productivity continues. One of them is through mutation breeding and in vitro culture. The purpose of this research was to look at the genetic diversity among napiergrasses using the Expressed Sequence Tag Simple Sequence Repeat (EST-SSR). This study used 14 SSR molecular markers. The results showed that mutant DNA of napiergrass can be clearly amplified by all the EST-SSR primers used. The average number of alleles was 4.57, the average frequency of the main allele was 42%, and the average value of gene diversity was 0.66. While the PIC average value was 0.60. There were five markers that were very informative and have PIC values ??above 0.7, among others, namely ICMP3045, ICMP3018, PSMP2090, PSMP2209, and PSMP2019. Phylogenetic analysis shows that 37 numbers of napiergrass mutants split into two main clusters at a coefficient of 0.56. The first cluster consists of 26 lines while the second cluster consists of 11 mutants. The parent napiergrass is in the first cluster. There are two pairs of mutants that have the same diversity, namely R20-11 with R 20-20-3 and R100-1 with R100-3.Mansyur M MansyurPanca Dewi MH KartiLuki AbdullahAli HusniPuji LestariMBI & UNS Soloarticleest-ssr, forage, genetic diversity, napiergrassBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	est-ssr, forage, genetic diversity, napiergrass Biology (General) QH301-705.5
spellingShingle	est-ssr, forage, genetic diversity, napiergrass Biology (General) QH301-705.5 Mansyur M Mansyur Panca Dewi MH Karti Luki Abdullah Ali Husni Puji Lestari Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)
description	Abstract. Mansyur, Karti PMH, Abdullah L, Husni A, Lestari P. 2019. Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR). Biodiversitas 20: 2403-2409. Napiergrass is one of the tropical grasses which has a very important role in developing ruminant livestock, its productivity is high and its nutritional content is quite good. Plant breeding to produce new varieties that have better productivity continues. One of them is through mutation breeding and in vitro culture. The purpose of this research was to look at the genetic diversity among napiergrasses using the Expressed Sequence Tag Simple Sequence Repeat (EST-SSR). This study used 14 SSR molecular markers. The results showed that mutant DNA of napiergrass can be clearly amplified by all the EST-SSR primers used. The average number of alleles was 4.57, the average frequency of the main allele was 42%, and the average value of gene diversity was 0.66. While the PIC average value was 0.60. There were five markers that were very informative and have PIC values ??above 0.7, among others, namely ICMP3045, ICMP3018, PSMP2090, PSMP2209, and PSMP2019. Phylogenetic analysis shows that 37 numbers of napiergrass mutants split into two main clusters at a coefficient of 0.56. The first cluster consists of 26 lines while the second cluster consists of 11 mutants. The parent napiergrass is in the first cluster. There are two pairs of mutants that have the same diversity, namely R20-11 with R 20-20-3 and R100-1 with R100-3.
format	article
author	Mansyur M Mansyur Panca Dewi MH Karti Luki Abdullah Ali Husni Puji Lestari
author_facet	Mansyur M Mansyur Panca Dewi MH Karti Luki Abdullah Ali Husni Puji Lestari
author_sort	Mansyur M Mansyur
title	Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)
title_short	Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)
title_full	Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)
title_fullStr	Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)
title_full_unstemmed	Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)
title_sort	genetic diversity of mutant napiergrass using expressed sequence tag simple sequence repeat (est-ssr)
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/b24672135f484065b62eab7857089cbe
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Genetic diversity of mutant napiergrass using Expressed Sequence Tag Simple Sequence Repeat (EST-SSR)

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