TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells

Mesenchymal stem cells (MSCs) are promising cells for regenerative medicine therapies because they can differentiate towards multiple cell lineages. However, the occurrence of cellular senescence and the acquiring of the senescence-associated secretory phenotype (SASP) limit their clinical use. Sinc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	C Voskamp, LA Anderson, WJLM Koevoet, S Barnhoorn, PG Mastroberardino, GJVM van Osch, R Narcisi
Formato:	article
Lenguaje:	EN
Publicado:	AO Research Institute Davos 2021
Materias:	mesenchymal stem cells cellular senescence secretory phenotype regenerative medicine metabolism Surgery RD1-811 Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/b24bd650f1c143828171c2dfd52711ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b24bd650f1c143828171c2dfd52711ad
record_format	dspace
spelling	oai:doaj.org-article:b24bd650f1c143828171c2dfd52711ad2021-11-26T10:42:16ZTWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells10.22203/eCM.v042a251473-2262https://doaj.org/article/b24bd650f1c143828171c2dfd52711ad2021-11-01T00:00:00Zhttps://www.ecmjournal.org/papers/vol042/pdf/v042a25.pdfhttps://doaj.org/toc/1473-2262Mesenchymal stem cells (MSCs) are promising cells for regenerative medicine therapies because they can differentiate towards multiple cell lineages. However, the occurrence of cellular senescence and the acquiring of the senescence-associated secretory phenotype (SASP) limit their clinical use. Since the transcription factor TWIST1 influences expansion of MSCs, its role in regulating cellular senescence was investigated. The present study demonstrated that silencing of TWIST1 in MSCs increased the occurrence of senescence, characterised by a SASP profile different from irradiation-induced senescent MSCs. Knowing that senescence alters cellular metabolism, cellular bioenergetics was monitored by using the Seahorse XF apparatus. Both TWIST1-silencing-induced and irradiation-induced senescent MSCs had a higher oxygen consumption rate compared to control MSCs, while TWIST1-silencing-induced senescent MSCs had a low extracellular acidification rate compared to irradiation-induced senescent MSCs. Overall, data indicated how TWIST1 regulation influenced senescence in MSCs and that TWIST1 silencing-induced senescence was characterised by a specific SASP profile and metabolic state.C VoskampLA AndersonWJLM KoevoetS BarnhoornPG MastroberardinoGJVM van OschR Narcisi AO Research Institute Davosarticlemesenchymal stem cellscellular senescencesecretory phenotyperegenerative medicinemetabolismSurgeryRD1-811Diseases of the musculoskeletal systemRC925-935ENEuropean Cells & Materials, Vol 42, Pp 401-414 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesenchymal stem cells cellular senescence secretory phenotype regenerative medicine metabolism Surgery RD1-811 Diseases of the musculoskeletal system RC925-935
spellingShingle	mesenchymal stem cells cellular senescence secretory phenotype regenerative medicine metabolism Surgery RD1-811 Diseases of the musculoskeletal system RC925-935 C Voskamp LA Anderson WJLM Koevoet S Barnhoorn PG Mastroberardino GJVM van Osch R Narcisi TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells
description	Mesenchymal stem cells (MSCs) are promising cells for regenerative medicine therapies because they can differentiate towards multiple cell lineages. However, the occurrence of cellular senescence and the acquiring of the senescence-associated secretory phenotype (SASP) limit their clinical use. Since the transcription factor TWIST1 influences expansion of MSCs, its role in regulating cellular senescence was investigated. The present study demonstrated that silencing of TWIST1 in MSCs increased the occurrence of senescence, characterised by a SASP profile different from irradiation-induced senescent MSCs. Knowing that senescence alters cellular metabolism, cellular bioenergetics was monitored by using the Seahorse XF apparatus. Both TWIST1-silencing-induced and irradiation-induced senescent MSCs had a higher oxygen consumption rate compared to control MSCs, while TWIST1-silencing-induced senescent MSCs had a low extracellular acidification rate compared to irradiation-induced senescent MSCs. Overall, data indicated how TWIST1 regulation influenced senescence in MSCs and that TWIST1 silencing-induced senescence was characterised by a specific SASP profile and metabolic state.
format	article
author	C Voskamp LA Anderson WJLM Koevoet S Barnhoorn PG Mastroberardino GJVM van Osch R Narcisi
author_facet	C Voskamp LA Anderson WJLM Koevoet S Barnhoorn PG Mastroberardino GJVM van Osch R Narcisi
author_sort	C Voskamp
title	TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells
title_short	TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells
title_full	TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells
title_fullStr	TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells
title_full_unstemmed	TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells
title_sort	twist1 controls cellular senescence and energy metabolism in mesenchymal stem cells
publisher	AO Research Institute Davos
publishDate	2021
url	https://doaj.org/article/b24bd650f1c143828171c2dfd52711ad
work_keys_str_mv	AT cvoskamp twist1controlscellularsenescenceandenergymetabolisminmesenchymalstemcells AT laanderson twist1controlscellularsenescenceandenergymetabolisminmesenchymalstemcells AT wjlmkoevoet twist1controlscellularsenescenceandenergymetabolisminmesenchymalstemcells AT sbarnhoorn twist1controlscellularsenescenceandenergymetabolisminmesenchymalstemcells AT pgmastroberardino twist1controlscellularsenescenceandenergymetabolisminmesenchymalstemcells AT gjvmvanosch twist1controlscellularsenescenceandenergymetabolisminmesenchymalstemcells AT rnarcisi twist1controlscellularsenescenceandenergymetabolisminmesenchymalstemcells
_version_	1718409615873409024

TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells

Ejemplares similares