Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk

Cryptomeria fortunei Hooibrenk is an important fast-growing coniferous timber species that is widely used in landscaping. Recently, research on timber quality has gained substantial attention in the field of tree breeding. Wood is the secondary xylem formed by the continuous inward division and diff...

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Autores principales:	Hailiang Hu, Zhenhao Guo, Junjie Yang, Jiebing Cui, Yingting Zhang, Jin Xu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Cryptomeria fortunei transcriptome miRNA vascular cambium regulatory genes Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/b24f9df4882f4213b7fec9d503d72ff7
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spelling	oai:doaj.org-article:b24f9df4882f4213b7fec9d503d72ff72021-11-18T14:56:19ZTranscriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk1664-462X10.3389/fpls.2021.751771https://doaj.org/article/b24f9df4882f4213b7fec9d503d72ff72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.751771/fullhttps://doaj.org/toc/1664-462XCryptomeria fortunei Hooibrenk is an important fast-growing coniferous timber species that is widely used in landscaping. Recently, research on timber quality has gained substantial attention in the field of tree breeding. Wood is the secondary xylem formed by the continuous inward division and differentiation of the vascular cambium; therefore, the development of the vascular cambium is particularly important for wood quality. In this study, we analyzed the transcriptomes of the cambial zone in C. fortunei during different developmental stages using Illumina HiSeq sequencing, focusing on general transcriptome and microRNA (miRNA) data. We performed functional annotation of the differentially expressed genes (DEGs) in the different stages identified by transcriptome sequencing and generated 15 miRNA libraries yielding 4.73 Gb of clean reads. The most common length of the filtered miRNAs was 21nt, accounting for 33.1% of the total filtered reads. We annotated a total of 32 known miRNA families. Some miRNAs played roles in hormone signal transduction (miR159, miR160, and miR166), growth and development (miR166 and miR396), and the coercion response (miR394 and miR395), and degradome sequencing showed potential cleavage sites between miRNAs and target genes. Differential expression of miRNAs and target genes and functional validation of the obtained transcriptome and miRNA data provide a theoretical basis for further elucidating the molecular mechanisms of cellular growth and differentiation, as well as wood formation in the vascular cambium, which will help improve the wood quality of C. fortunei.Hailiang HuHailiang HuHailiang HuZhenhao GuoZhenhao GuoZhenhao GuoZhenhao GuoJunjie YangJunjie YangJunjie YangJiebing CuiJiebing CuiJiebing CuiYingting ZhangYingting ZhangYingting ZhangJin XuJin XuJin XuFrontiers Media S.A.articleCryptomeria fortuneitranscriptomemiRNAvascular cambiumregulatory genesPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cryptomeria fortunei transcriptome miRNA vascular cambium regulatory genes Plant culture SB1-1110
spellingShingle	Cryptomeria fortunei transcriptome miRNA vascular cambium regulatory genes Plant culture SB1-1110 Hailiang Hu Hailiang Hu Hailiang Hu Zhenhao Guo Zhenhao Guo Zhenhao Guo Zhenhao Guo Junjie Yang Junjie Yang Junjie Yang Jiebing Cui Jiebing Cui Jiebing Cui Yingting Zhang Yingting Zhang Yingting Zhang Jin Xu Jin Xu Jin Xu Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk
description	Cryptomeria fortunei Hooibrenk is an important fast-growing coniferous timber species that is widely used in landscaping. Recently, research on timber quality has gained substantial attention in the field of tree breeding. Wood is the secondary xylem formed by the continuous inward division and differentiation of the vascular cambium; therefore, the development of the vascular cambium is particularly important for wood quality. In this study, we analyzed the transcriptomes of the cambial zone in C. fortunei during different developmental stages using Illumina HiSeq sequencing, focusing on general transcriptome and microRNA (miRNA) data. We performed functional annotation of the differentially expressed genes (DEGs) in the different stages identified by transcriptome sequencing and generated 15 miRNA libraries yielding 4.73 Gb of clean reads. The most common length of the filtered miRNAs was 21nt, accounting for 33.1% of the total filtered reads. We annotated a total of 32 known miRNA families. Some miRNAs played roles in hormone signal transduction (miR159, miR160, and miR166), growth and development (miR166 and miR396), and the coercion response (miR394 and miR395), and degradome sequencing showed potential cleavage sites between miRNAs and target genes. Differential expression of miRNAs and target genes and functional validation of the obtained transcriptome and miRNA data provide a theoretical basis for further elucidating the molecular mechanisms of cellular growth and differentiation, as well as wood formation in the vascular cambium, which will help improve the wood quality of C. fortunei.
format	article
author	Hailiang Hu Hailiang Hu Hailiang Hu Zhenhao Guo Zhenhao Guo Zhenhao Guo Zhenhao Guo Junjie Yang Junjie Yang Junjie Yang Jiebing Cui Jiebing Cui Jiebing Cui Yingting Zhang Yingting Zhang Yingting Zhang Jin Xu Jin Xu Jin Xu
author_facet	Hailiang Hu Hailiang Hu Hailiang Hu Zhenhao Guo Zhenhao Guo Zhenhao Guo Zhenhao Guo Junjie Yang Junjie Yang Junjie Yang Jiebing Cui Jiebing Cui Jiebing Cui Yingting Zhang Yingting Zhang Yingting Zhang Jin Xu Jin Xu Jin Xu
author_sort	Hailiang Hu
title	Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk
title_short	Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk
title_full	Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk
title_fullStr	Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk
title_full_unstemmed	Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk
title_sort	transcriptome and microrna sequencing identified mirnas and target genes in different developmental stages of the vascular cambium in cryptomeria fortunei hooibrenk
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b24f9df4882f4213b7fec9d503d72ff7
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Transcriptome and microRNA Sequencing Identified miRNAs and Target Genes in Different Developmental Stages of the Vascular Cambium in Cryptomeria fortunei Hooibrenk

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