Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle

This study aimed to diagnose Lumpy skin desease by isolation and identification of LSD from suspected naturally infected cattle and to study its effect on immuno-biochemical parameters, inflammatory and oxidative stress markers. The study was conducted on 25 cattle, 1-3 years age from two cities in...

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Autores principales:	Morcos Ibrahim Yanni, Ibtisam Mohamad Gamal Elden, Nahed Abd Elhafeeze Kamoura, Mohamed Abdu Ibrahim
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	lumpy skin disease virus (lsdv) sequence cattle phagocytic activity immuno-biochemical Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.140085 https://doaj.org/article/b264434a6a364ddc9cb78cf3e417a736
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spelling	oai:doaj.org-article:b264434a6a364ddc9cb78cf3e417a7362021-12-02T16:34:28ZVirological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattlehttps://dx.doi.org/10.21608/javs.2021.1400851687-40722090-3308https://doaj.org/article/b264434a6a364ddc9cb78cf3e417a7362021-01-01T00:00:00Zhttps://javs.journals.ekb.eg/article_140085.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308This study aimed to diagnose Lumpy skin desease by isolation and identification of LSD from suspected naturally infected cattle and to study its effect on immuno-biochemical parameters, inflammatory and oxidative stress markers. The study was conducted on 25 cattle, 1-3 years age from two cities in the Sharkia governorate. Ten clinically healthy cattle represented the control group and fifteen suspected diseased cases by LSD represented the infected group. The clinical signs of suspected cases showed anorexia, skin nodules all over the body, fever above 40˚C, edema in forelimbs and dewlap, enlarged superficial lymph nodes, retarded milk production, and abortion in some cases. The virological examination of LSDV from the samples of skin nodules was done. Isolation from skin nodules was done on MDBK cell line and embryonated chiken egg through chorioallantoic membrane route followed by identification using fluorescence technique . Molecular studies including phylogenetic analysis for three positive isolates showed similarity to previously locally isolated strains in Egypt and close relation to strains of SPPV. Immuno-biochemical results revealed a significant increase in aspartate aminotransferase , alanine aminotransferase , alkaline phosphatase , Malondialdehyde , Tumor necrosis factor , Interleukin4,immunoglobulin M and immunoglobulin G . Meanwhile, a significant decrease in total protein, albumin, globulin, Glutathione peroxidase, phagocytic assay, calcium, inorganic phosphorous, sodium, and potassium with a non-significant change in magnesium was seen when compared to control ones. It could be concluded that the infection with LSD in cattle induce severe effects on immuno-biochemical, antioxidant parameters, and inflammatory markers causing great economic lossMorcos Ibrahim YanniIbtisam Mohamad Gamal EldenNahed Abd Elhafeeze KamouraMohamed Abdu IbrahimEgyptian Society for Animal Managementarticlelumpy skin disease virus (lsdv)sequencecattlephagocytic activityimmuno-biochemicalZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 1, Pp 28-37 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lumpy skin disease virus (lsdv) sequence cattle phagocytic activity immuno-biochemical Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	lumpy skin disease virus (lsdv) sequence cattle phagocytic activity immuno-biochemical Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Morcos Ibrahim Yanni Ibtisam Mohamad Gamal Elden Nahed Abd Elhafeeze Kamoura Mohamed Abdu Ibrahim Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle
description	This study aimed to diagnose Lumpy skin desease by isolation and identification of LSD from suspected naturally infected cattle and to study its effect on immuno-biochemical parameters, inflammatory and oxidative stress markers. The study was conducted on 25 cattle, 1-3 years age from two cities in the Sharkia governorate. Ten clinically healthy cattle represented the control group and fifteen suspected diseased cases by LSD represented the infected group. The clinical signs of suspected cases showed anorexia, skin nodules all over the body, fever above 40˚C, edema in forelimbs and dewlap, enlarged superficial lymph nodes, retarded milk production, and abortion in some cases. The virological examination of LSDV from the samples of skin nodules was done. Isolation from skin nodules was done on MDBK cell line and embryonated chiken egg through chorioallantoic membrane route followed by identification using fluorescence technique . Molecular studies including phylogenetic analysis for three positive isolates showed similarity to previously locally isolated strains in Egypt and close relation to strains of SPPV. Immuno-biochemical results revealed a significant increase in aspartate aminotransferase , alanine aminotransferase , alkaline phosphatase , Malondialdehyde , Tumor necrosis factor , Interleukin4,immunoglobulin M and immunoglobulin G . Meanwhile, a significant decrease in total protein, albumin, globulin, Glutathione peroxidase, phagocytic assay, calcium, inorganic phosphorous, sodium, and potassium with a non-significant change in magnesium was seen when compared to control ones. It could be concluded that the infection with LSD in cattle induce severe effects on immuno-biochemical, antioxidant parameters, and inflammatory markers causing great economic loss
format	article
author	Morcos Ibrahim Yanni Ibtisam Mohamad Gamal Elden Nahed Abd Elhafeeze Kamoura Mohamed Abdu Ibrahim
author_facet	Morcos Ibrahim Yanni Ibtisam Mohamad Gamal Elden Nahed Abd Elhafeeze Kamoura Mohamed Abdu Ibrahim
author_sort	Morcos Ibrahim Yanni
title	Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle
title_short	Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle
title_full	Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle
title_fullStr	Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle
title_full_unstemmed	Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle
title_sort	virological, molecular and immuno-biochemical studies of lumpy skin disease in naturally infected cattle
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.140085 https://doaj.org/article/b264434a6a364ddc9cb78cf3e417a736
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Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle

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