Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling

Abstract Downregulation of Mucin 2 (MUC2) expression is associated with early carcinogenesis events in colon cancer. MUC2 plays a role in the progression of colon cancer, and reduced MUC2 protein expression correlates with increased interleukin-6 (IL-6) expression. However, the interaction between M...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hui-Ping Hsu, Ming-Derg Lai, Jenq-Chang Lee, Meng-Chi Yen, Tzu-Yang Weng, Wei-Ching Chen, Jung-Hua Fang, Yi-Ling Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b26b679bc14e475ebaf6edd19ce6bd06
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b26b679bc14e475ebaf6edd19ce6bd06
record_format	dspace
spelling	oai:doaj.org-article:b26b679bc14e475ebaf6edd19ce6bd062021-12-02T12:30:43ZMucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling10.1038/s41598-017-04952-72045-2322https://doaj.org/article/b26b679bc14e475ebaf6edd19ce6bd062017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04952-7https://doaj.org/toc/2045-2322Abstract Downregulation of Mucin 2 (MUC2) expression is associated with early carcinogenesis events in colon cancer. MUC2 plays a role in the progression of colon cancer, and reduced MUC2 protein expression correlates with increased interleukin-6 (IL-6) expression. However, the interaction between MUC2 and IL-6 in colorectal cancer metastasis remains unclear. We systematically analyzed MUC2 and IL-6 expression and determined the survival of cancer patients with high or low MUC2 and IL-6 expression using the Oncomine and PrognoScan databases, respectively. This analysis identified downregulation of MUC2 and overexpression of IL-6 in colon cancer but not in normal colon tissue, and this expression pattern was correlated with poor survival of colon cancer patients. We examined the effects of MUC2 on colon cancer metastasis and used vector-mediated application of short hairpin RNA (shRNA) to suppress MUC2 expression. MUC2 suppressed the migration of colon cancer cells in vitro and dramatically diminished liver metastases in vivo. Treatment with IL-6 increased signal transducer and activator of transcription 3 (STAT3) phosphorylation, promoted checkpoint kinase 2 (Chk2) activation, attenuated cAMP response element-binding protein (CREB) phosphorylation, and suppressed E-cadherin protein expression in MUC2-silenced HT-29 cancer cells. Most importantly, MUC2 is a potential prognostic indicator for colon cancer.Hui-Ping HsuMing-Derg LaiJenq-Chang LeeMeng-Chi YenTzu-Yang WengWei-Ching ChenJung-Hua FangYi-Ling ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui-Ping Hsu Ming-Derg Lai Jenq-Chang Lee Meng-Chi Yen Tzu-Yang Weng Wei-Ching Chen Jung-Hua Fang Yi-Ling Chen Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling
description	Abstract Downregulation of Mucin 2 (MUC2) expression is associated with early carcinogenesis events in colon cancer. MUC2 plays a role in the progression of colon cancer, and reduced MUC2 protein expression correlates with increased interleukin-6 (IL-6) expression. However, the interaction between MUC2 and IL-6 in colorectal cancer metastasis remains unclear. We systematically analyzed MUC2 and IL-6 expression and determined the survival of cancer patients with high or low MUC2 and IL-6 expression using the Oncomine and PrognoScan databases, respectively. This analysis identified downregulation of MUC2 and overexpression of IL-6 in colon cancer but not in normal colon tissue, and this expression pattern was correlated with poor survival of colon cancer patients. We examined the effects of MUC2 on colon cancer metastasis and used vector-mediated application of short hairpin RNA (shRNA) to suppress MUC2 expression. MUC2 suppressed the migration of colon cancer cells in vitro and dramatically diminished liver metastases in vivo. Treatment with IL-6 increased signal transducer and activator of transcription 3 (STAT3) phosphorylation, promoted checkpoint kinase 2 (Chk2) activation, attenuated cAMP response element-binding protein (CREB) phosphorylation, and suppressed E-cadherin protein expression in MUC2-silenced HT-29 cancer cells. Most importantly, MUC2 is a potential prognostic indicator for colon cancer.
format	article
author	Hui-Ping Hsu Ming-Derg Lai Jenq-Chang Lee Meng-Chi Yen Tzu-Yang Weng Wei-Ching Chen Jung-Hua Fang Yi-Ling Chen
author_facet	Hui-Ping Hsu Ming-Derg Lai Jenq-Chang Lee Meng-Chi Yen Tzu-Yang Weng Wei-Ching Chen Jung-Hua Fang Yi-Ling Chen
author_sort	Hui-Ping Hsu
title	Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling
title_short	Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling
title_full	Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling
title_fullStr	Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling
title_full_unstemmed	Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling
title_sort	mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b26b679bc14e475ebaf6edd19ce6bd06
work_keys_str_mv	AT huipinghsu mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling AT mingderglai mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling AT jenqchanglee mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling AT mengchiyen mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling AT tzuyangweng mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling AT weichingchen mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling AT junghuafang mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling AT yilingchen mucin2silencingpromotescoloncancermetastasisthroughinterleukin6signaling
_version_	1718394355542130688

Mucin 2 silencing promotes colon cancer metastasis through interleukin-6 signaling

Ejemplares similares