Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia

Background: antibiotic resistance is spreading worldwide. It is important to evaluate whether foods of animal origin constitute a reservoir of resistance genes. Objective: the aim of the present study was to assess the occurrence and characterize extended-spectrum beta-lactamase (ESBL)-producing Ent...

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Autores principales:	Laura Vásquez-Jaramillo, Nicolás F. Ramírez, Ömer Akineden, Jorge A. Fernández Silva
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2017
Materias:	antibiotic resistance ctx-m-12a livestock public health raw milk Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/b26b7fde2d22423b90a09541f1fd6964
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spelling	oai:doaj.org-article:b26b7fde2d22423b90a09541f1fd69642021-11-26T19:19:23ZPresence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia2256-295810.17533/udea.rccp.v30n2a01https://doaj.org/article/b26b7fde2d22423b90a09541f1fd69642017-04-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/327056https://doaj.org/toc/2256-2958Background: antibiotic resistance is spreading worldwide. It is important to evaluate whether foods of animal origin constitute a reservoir of resistance genes. Objective: the aim of the present study was to assess the occurrence and characterize extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae from bulk-tank milk samples of dairy farms located in Entrerríos, Antioquia (Colombia). Methods: a total of 120 randomly selected raw milk samples (one bulk-tank milk sample per dairy farm) were collected between September and October, 2013. A commercial chromogenic agar was used for screening presumptive ESBL-E. Identification of genus and species of isolates was performed with a commercial biochemical identification kit. The ESBL production was confirmed using the double disc synergy test. An antimicrobial susceptibility test was performed by the agar disc diffusion method and the automatized broth microdilution method. The ESBL-positive isolates were analyzed for the presence of bla genes by polymerase chain reaction (PCR) and sequencing. For the exploration of risk factors, information on dairy farm management practices was recorded using a questionnaire and the associations of predictors and results were tested with a logistic regression analysis. Results: the ESBL-E were isolated from 3.3% (4/120; CI 95%: 3-3.5%) of the samples. Farm size was the only factor associated with the presence of ESBL-E (OR = 11.5; CI 95%: 1.14-115.54; p = 0.038). All isolates were resistant to several antibiotics and harbored blaCTX-M-96 (alternate name CTX-M-12a) enzymes. Conclusion: although apparent frequency of ESBL-E was low, the presence of these resistant bacteria in milk may constitute a public health risk and should be further investigated. . Resumo Palavras chave: CTX-M-12A, gado, leite crua, resistência aos antibióticos, saúde pública.Laura Vásquez-JaramilloNicolás F. RamírezÖmer AkinedenJorge A. Fernández SilvaUniversidad de Antioquiaarticleantibiotic resistancectx-m-12alivestockpublic healthraw milkAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 30, Iss 2, Pp 85-100 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance ctx-m-12a livestock public health raw milk Animal culture SF1-1100
spellingShingle	antibiotic resistance ctx-m-12a livestock public health raw milk Animal culture SF1-1100 Laura Vásquez-Jaramillo Nicolás F. Ramírez Ömer Akineden Jorge A. Fernández Silva Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia
description	Background: antibiotic resistance is spreading worldwide. It is important to evaluate whether foods of animal origin constitute a reservoir of resistance genes. Objective: the aim of the present study was to assess the occurrence and characterize extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae from bulk-tank milk samples of dairy farms located in Entrerríos, Antioquia (Colombia). Methods: a total of 120 randomly selected raw milk samples (one bulk-tank milk sample per dairy farm) were collected between September and October, 2013. A commercial chromogenic agar was used for screening presumptive ESBL-E. Identification of genus and species of isolates was performed with a commercial biochemical identification kit. The ESBL production was confirmed using the double disc synergy test. An antimicrobial susceptibility test was performed by the agar disc diffusion method and the automatized broth microdilution method. The ESBL-positive isolates were analyzed for the presence of bla genes by polymerase chain reaction (PCR) and sequencing. For the exploration of risk factors, information on dairy farm management practices was recorded using a questionnaire and the associations of predictors and results were tested with a logistic regression analysis. Results: the ESBL-E were isolated from 3.3% (4/120; CI 95%: 3-3.5%) of the samples. Farm size was the only factor associated with the presence of ESBL-E (OR = 11.5; CI 95%: 1.14-115.54; p = 0.038). All isolates were resistant to several antibiotics and harbored blaCTX-M-96 (alternate name CTX-M-12a) enzymes. Conclusion: although apparent frequency of ESBL-E was low, the presence of these resistant bacteria in milk may constitute a public health risk and should be further investigated. . Resumo Palavras chave: CTX-M-12A, gado, leite crua, resistência aos antibióticos, saúde pública.
format	article
author	Laura Vásquez-Jaramillo Nicolás F. Ramírez Ömer Akineden Jorge A. Fernández Silva
author_facet	Laura Vásquez-Jaramillo Nicolás F. Ramírez Ömer Akineden Jorge A. Fernández Silva
author_sort	Laura Vásquez-Jaramillo
title	Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia
title_short	Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia
title_full	Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia
title_fullStr	Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia
title_full_unstemmed	Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia
title_sort	presence of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in bulk-tank milk of bovine dairy farms in antioquia, colombia
publisher	Universidad de Antioquia
publishDate	2017
url	https://doaj.org/article/b26b7fde2d22423b90a09541f1fd6964
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Presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in bulk-tank milk of bovine dairy farms in Antioquia, Colombia

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