Centrifugal force assessment in ram sperm: identifying species-specific impact

Abstract Background Centrifugation is routinely employed in handling the ejaculates of some species, but it is not part of the commonly used protocols in ram. However, the development and implementation of new assisted reproductive technologies, alternative preservation models based on washing sperm...

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Autores principales:	Marta Neila-Montero, Marta F. Riesco, Mercedes Alvarez, Rafael Montes-Garrido, Juan Carlos Boixo, Paulino de Paz, Luis Anel-Lopez, Luis Anel
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Centrifugation Ejaculate handling Ovine Semen Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/b273096544cc469f848697ac99a7d6c0
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spelling	oai:doaj.org-article:b273096544cc469f848697ac99a7d6c02021-11-08T11:03:51ZCentrifugal force assessment in ram sperm: identifying species-specific impact10.1186/s13028-021-00609-81751-0147https://doaj.org/article/b273096544cc469f848697ac99a7d6c02021-11-01T00:00:00Zhttps://doi.org/10.1186/s13028-021-00609-8https://doaj.org/toc/1751-0147Abstract Background Centrifugation is routinely employed in handling the ejaculates of some species, but it is not part of the commonly used protocols in ram. However, the development and implementation of new assisted reproductive technologies, alternative preservation models based on washing sperm from a cellular ageing-accelerating substance such as the seminal plasma, and basic studies in spermatology is associated with the use of centrifugation. This requires a specific evaluation of the centrifugation protocols considering the species-specific relationship with the potential damage produced by this procedure. No previous studies have determined the effect of different centrifugation forces on ram sperm. Therefore, we aimed to assess the performance of three centrifugal forces (600×g, 3000×g, and 6000×g for 10 min at room temperature) and their effects on ram sperm motility and functionality. Results Sperm motility and functionality parameters were assessed at 0 h and after 2 h of incubation at 37 °C. As expected, a higher cell packaging degree was obtained at high centrifugation forces (P ≤ 0.0001). Cell packaging was unstable at all centrifugal forces. Thus, there was a high cell resuspension rate after less than 2 min. Regarding sperm quality, there was a change in movement pattern of 3000×g and 6000×g centrifuged sperm after 2 h of incubation at 37 °C, characterized by an increase in rapid progressive motility, linearity, straightness, and beat frequency, and a decrease in medium progressive motility, curvilinear velocity, path velocity, and head lateral amplitude. Non-significant differences were obtained among the different treatments concerning the total viability. However, we observed a significant increase (P ≤ 0.05) in the percentage of viable apoptotic sperm in the samples centrifuged at 6000×g at 0 h. Conclusions Centrifugal forces equal to or greater than 3000×g induced some deleterious effects in ram sperm quality, and lower forces did not provide a successful cell packaging degree.Marta Neila-MonteroMarta F. RiescoMercedes AlvarezRafael Montes-GarridoJuan Carlos BoixoPaulino de PazLuis Anel-LopezLuis AnelBMCarticleCentrifugationEjaculate handlingOvineSemenVeterinary medicineSF600-1100ENActa Veterinaria Scandinavica, Vol 63, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Centrifugation Ejaculate handling Ovine Semen Veterinary medicine SF600-1100
spellingShingle	Centrifugation Ejaculate handling Ovine Semen Veterinary medicine SF600-1100 Marta Neila-Montero Marta F. Riesco Mercedes Alvarez Rafael Montes-Garrido Juan Carlos Boixo Paulino de Paz Luis Anel-Lopez Luis Anel Centrifugal force assessment in ram sperm: identifying species-specific impact
description	Abstract Background Centrifugation is routinely employed in handling the ejaculates of some species, but it is not part of the commonly used protocols in ram. However, the development and implementation of new assisted reproductive technologies, alternative preservation models based on washing sperm from a cellular ageing-accelerating substance such as the seminal plasma, and basic studies in spermatology is associated with the use of centrifugation. This requires a specific evaluation of the centrifugation protocols considering the species-specific relationship with the potential damage produced by this procedure. No previous studies have determined the effect of different centrifugation forces on ram sperm. Therefore, we aimed to assess the performance of three centrifugal forces (600×g, 3000×g, and 6000×g for 10 min at room temperature) and their effects on ram sperm motility and functionality. Results Sperm motility and functionality parameters were assessed at 0 h and after 2 h of incubation at 37 °C. As expected, a higher cell packaging degree was obtained at high centrifugation forces (P ≤ 0.0001). Cell packaging was unstable at all centrifugal forces. Thus, there was a high cell resuspension rate after less than 2 min. Regarding sperm quality, there was a change in movement pattern of 3000×g and 6000×g centrifuged sperm after 2 h of incubation at 37 °C, characterized by an increase in rapid progressive motility, linearity, straightness, and beat frequency, and a decrease in medium progressive motility, curvilinear velocity, path velocity, and head lateral amplitude. Non-significant differences were obtained among the different treatments concerning the total viability. However, we observed a significant increase (P ≤ 0.05) in the percentage of viable apoptotic sperm in the samples centrifuged at 6000×g at 0 h. Conclusions Centrifugal forces equal to or greater than 3000×g induced some deleterious effects in ram sperm quality, and lower forces did not provide a successful cell packaging degree.
format	article
author	Marta Neila-Montero Marta F. Riesco Mercedes Alvarez Rafael Montes-Garrido Juan Carlos Boixo Paulino de Paz Luis Anel-Lopez Luis Anel
author_facet	Marta Neila-Montero Marta F. Riesco Mercedes Alvarez Rafael Montes-Garrido Juan Carlos Boixo Paulino de Paz Luis Anel-Lopez Luis Anel
author_sort	Marta Neila-Montero
title	Centrifugal force assessment in ram sperm: identifying species-specific impact
title_short	Centrifugal force assessment in ram sperm: identifying species-specific impact
title_full	Centrifugal force assessment in ram sperm: identifying species-specific impact
title_fullStr	Centrifugal force assessment in ram sperm: identifying species-specific impact
title_full_unstemmed	Centrifugal force assessment in ram sperm: identifying species-specific impact
title_sort	centrifugal force assessment in ram sperm: identifying species-specific impact
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b273096544cc469f848697ac99a7d6c0
work_keys_str_mv	AT martaneilamontero centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact AT martafriesco centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact AT mercedesalvarez centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact AT rafaelmontesgarrido centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact AT juancarlosboixo centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact AT paulinodepaz centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact AT luisanellopez centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact AT luisanel centrifugalforceassessmentinramspermidentifyingspeciesspecificimpact
_version_	1718442461341155328

Centrifugal force assessment in ram sperm: identifying species-specific impact

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