Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.

Understanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature dur...

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Autores principales:	Valeria Paula Carreira, Marcos A Imberti, Julián Mensch, Juan José Fanara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b2773ea07eb647a0a217cae98681e30f
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spelling	oai:doaj.org-article:b2773ea07eb647a0a217cae98681e30f2021-11-18T09:01:55ZGene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.1932-620310.1371/journal.pone.0070851https://doaj.org/article/b2773ea07eb647a0a217cae98681e30f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936253/?tool=EBIhttps://doaj.org/toc/1932-6203Understanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature during ontogenesis is an important environmental factor affecting development and phenotypic variation in holometabolous insects. In spite of the importance of phenotypic plasticity and genotype by environment interaction (GEI) for fitness related traits, its genetic basis has remained elusive. In this context, we analyzed five different adult morphological traits (face width, head width, thorax length, wing size and wing shape) in 42 co-isogenic single P-element insertional lines of Drosophila melanogaster raised at 17°C and 25°C. Our analyses showed that all lines differed from the control for at least one trait in males or females at either temperature. However, no line showed those differences for all traits in both sexes and temperatures simultaneously. In this sense, the most pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses also revealed extensive genetic variation for all the characters mostly indicated by strong GEIs. Further, our results indicate that GEIs were predominantly explained by changes in ranking order in all cases suggesting that a moderate number of genes are involved in the expression of each character at both temperatures. Most lines displayed a plastic response for at least one trait in either sex. In this regard, P-element insertions affecting plasticity of a large number of traits were associated to the candidate genes Btk29A, CG43340, Drak and jim. Further studies will help to elucidate the relevance of these genes on the morphogenesis of different body structures in natural populations of D. melanogaster.Valeria Paula CarreiraMarcos A ImbertiJulián MenschJuan José FanaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70851 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valeria Paula Carreira Marcos A Imberti Julián Mensch Juan José Fanara Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.
description	Understanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature during ontogenesis is an important environmental factor affecting development and phenotypic variation in holometabolous insects. In spite of the importance of phenotypic plasticity and genotype by environment interaction (GEI) for fitness related traits, its genetic basis has remained elusive. In this context, we analyzed five different adult morphological traits (face width, head width, thorax length, wing size and wing shape) in 42 co-isogenic single P-element insertional lines of Drosophila melanogaster raised at 17°C and 25°C. Our analyses showed that all lines differed from the control for at least one trait in males or females at either temperature. However, no line showed those differences for all traits in both sexes and temperatures simultaneously. In this sense, the most pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses also revealed extensive genetic variation for all the characters mostly indicated by strong GEIs. Further, our results indicate that GEIs were predominantly explained by changes in ranking order in all cases suggesting that a moderate number of genes are involved in the expression of each character at both temperatures. Most lines displayed a plastic response for at least one trait in either sex. In this regard, P-element insertions affecting plasticity of a large number of traits were associated to the candidate genes Btk29A, CG43340, Drak and jim. Further studies will help to elucidate the relevance of these genes on the morphogenesis of different body structures in natural populations of D. melanogaster.
format	article
author	Valeria Paula Carreira Marcos A Imberti Julián Mensch Juan José Fanara
author_facet	Valeria Paula Carreira Marcos A Imberti Julián Mensch Juan José Fanara
author_sort	Valeria Paula Carreira
title	Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.
title_short	Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.
title_full	Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.
title_fullStr	Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.
title_full_unstemmed	Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.
title_sort	gene-by-temperature interactions and candidate plasticity genes for morphological traits in drosophila melanogaster.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b2773ea07eb647a0a217cae98681e30f
work_keys_str_mv	AT valeriapaulacarreira genebytemperatureinteractionsandcandidateplasticitygenesformorphologicaltraitsindrosophilamelanogaster AT marcosaimberti genebytemperatureinteractionsandcandidateplasticitygenesformorphologicaltraitsindrosophilamelanogaster AT julianmensch genebytemperatureinteractionsandcandidateplasticitygenesformorphologicaltraitsindrosophilamelanogaster AT juanjosefanara genebytemperatureinteractionsandcandidateplasticitygenesformorphologicaltraitsindrosophilamelanogaster
_version_	1718421043828228096

Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.

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