Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ

Abundant evidence of microplastics (MP) found in the environment, and its toxicity effect in animals calls for human-related research. However, well-established quantitative controlled studies on the potential route of human exposure to MP are still sparse. MP count, size and morphology in 15 polyla...

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Autores principales:	Shujuan Chen, Yue Li, Christopher Mawhorter, Saamon Legoski
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	beverage containers microplastics microscopy morphology quantification Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/b27bb1bdf4654296acdebdd105adc082
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spelling	oai:doaj.org-article:b27bb1bdf4654296acdebdd105adc0822021-11-06T05:46:01ZQuantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ1477-89201996-782910.2166/wh.2020.171https://doaj.org/article/b27bb1bdf4654296acdebdd105adc0822021-02-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/1/79https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829Abundant evidence of microplastics (MP) found in the environment, and its toxicity effect in animals calls for human-related research. However, well-established quantitative controlled studies on the potential route of human exposure to MP are still sparse. MP count, size and morphology in 15 polylactic acid (PLA)-lined plastic cups and 15 PLA-lined paper cups were examined using Nile Red fluorescence tagging, microscopic photography, and morphology assessment and quantification based on ImageJ. In the plastic cups, the count and area of MP fibers were found to be significantly higher compared with blanks (p < 0.05), but not MP particles or total MP. In paper cups, count or area was not significantly different in terms of MP particle, MP fibers or total MP. No interesting trend was observed in the distribution regarding the size of MP particles or fibers. These results indicate that selected paper cups and plastic cups could be considered as safe beverage containers, but further research on the toxicological effects of MPs in different morphologies released from plastic cups on human health is needed.Shujuan ChenYue LiChristopher MawhorterSaamon LegoskiIWA Publishingarticlebeverage containersmicroplasticsmicroscopymorphologyquantificationPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 1, Pp 79-88 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	beverage containers microplastics microscopy morphology quantification Public aspects of medicine RA1-1270
spellingShingle	beverage containers microplastics microscopy morphology quantification Public aspects of medicine RA1-1270 Shujuan Chen Yue Li Christopher Mawhorter Saamon Legoski Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
description	Abundant evidence of microplastics (MP) found in the environment, and its toxicity effect in animals calls for human-related research. However, well-established quantitative controlled studies on the potential route of human exposure to MP are still sparse. MP count, size and morphology in 15 polylactic acid (PLA)-lined plastic cups and 15 PLA-lined paper cups were examined using Nile Red fluorescence tagging, microscopic photography, and morphology assessment and quantification based on ImageJ. In the plastic cups, the count and area of MP fibers were found to be significantly higher compared with blanks (p < 0.05), but not MP particles or total MP. In paper cups, count or area was not significantly different in terms of MP particle, MP fibers or total MP. No interesting trend was observed in the distribution regarding the size of MP particles or fibers. These results indicate that selected paper cups and plastic cups could be considered as safe beverage containers, but further research on the toxicological effects of MPs in different morphologies released from plastic cups on human health is needed.
format	article
author	Shujuan Chen Yue Li Christopher Mawhorter Saamon Legoski
author_facet	Shujuan Chen Yue Li Christopher Mawhorter Saamon Legoski
author_sort	Shujuan Chen
title	Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
title_short	Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
title_full	Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
title_fullStr	Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
title_full_unstemmed	Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
title_sort	quantification of microplastics by count, size and morphology in beverage containers using nile red and imagej
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/b27bb1bdf4654296acdebdd105adc082
work_keys_str_mv	AT shujuanchen quantificationofmicroplasticsbycountsizeandmorphologyinbeveragecontainersusingnileredandimagej AT yueli quantificationofmicroplasticsbycountsizeandmorphologyinbeveragecontainersusingnileredandimagej AT christophermawhorter quantificationofmicroplasticsbycountsizeandmorphologyinbeveragecontainersusingnileredandimagej AT saamonlegoski quantificationofmicroplasticsbycountsizeandmorphologyinbeveragecontainersusingnileredandimagej
_version_	1718443875503177728

Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ

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