The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures

Abstract Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are locate...

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Autores principales:	B. Mir, X. Solés, C. González, N. Escaja
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b288ee1eb6af4dbaae3b1e08d253ca64
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spelling	oai:doaj.org-article:b288ee1eb6af4dbaae3b1e08d253ca642021-12-02T15:04:54ZThe effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures10.1038/s41598-017-02723-y2045-2322https://doaj.org/article/b288ee1eb6af4dbaae3b1e08d253ca642017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02723-yhttps://doaj.org/toc/2045-2322Abstract Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C+ stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C+ or psC:psC+ hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure.B. MirX. SolésC. GonzálezN. EscajaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q B. Mir X. Solés C. González N. Escaja The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
description	Abstract Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C+ stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C+ or psC:psC+ hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure.
format	article
author	B. Mir X. Solés C. González N. Escaja
author_facet	B. Mir X. Solés C. González N. Escaja
author_sort	B. Mir
title	The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_short	The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_full	The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_fullStr	The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_full_unstemmed	The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_sort	effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b288ee1eb6af4dbaae3b1e08d253ca64
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The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures

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