Reconstitution of the complete pathway of ITS2 processing at the pre-ribosome

Excision of internal transcribed spacer 2 (ITS2) within eukaryotic pre-ribosomal RNA is essential for ribosome function. Here, the authors reconstitute the entire cycle of ITS2 processing in vitro using purified components, providing insights into the cleavage process and demonstrating that 26S pre-...

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Autores principales: Lisa Fromm, Sebastian Falk, Dirk Flemming, Jan Michael Schuller, Matthias Thoms, Elena Conti, Ed Hurt
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/b28d8e98866847018326ad389cebdf76
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