Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles

Carmen Irina Vamanu1, Paul Johan H&oslash;l2, Zouhir Ekeland Allouni2, Said Elsayed3, Nils Roar Gjerdet11Departments of Oral Sciences &ndash; Dental Biomaterials, Faculty of Dentistry, University of Bergen, Bergen, Norway; 2Orthopedic Biomaterials, Helse Bergen Haukeland University H...

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Autores principales:	Carmen Irina Vamanu, Paul Johan H&oslash;l, Zouhir Ekel, Allouni, Said Elsayed, Nils Roar Gjerdet
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b296e822f6074577a9d393351147e3de
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spelling	oai:doaj.org-article:b296e822f6074577a9d393351147e3de2021-12-02T00:02:34ZFormation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles1176-91141178-2013https://doaj.org/article/b296e822f6074577a9d393351147e3de2008-03-01T00:00:00Zhttp://www.dovepress.com/formation-of-potential-titanium-antigens-based-on-protein-binding-to-t-a738https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Carmen Irina Vamanu1, Paul Johan H&oslash;l2, Zouhir Ekeland Allouni2, Said Elsayed3, Nils Roar Gjerdet11Departments of Oral Sciences &ndash; Dental Biomaterials, Faculty of Dentistry, University of Bergen, Bergen, Norway; 2Orthopedic Biomaterials, Helse Bergen Haukeland University Hospital, Bergen, Norway; 3Department of Medicine, University of Bergen, Bergen, NorwayAbstract: Degradation products of titanium implants include free ions, organo-metallic complexes, and particles, ranging from nano to macro sizes. The biological effects, especially of nanoparticles, is yet unknown. The main objective of this study was to develop Ti-protein antigens in physiological solutions that can be used in testing of cellular responses. For this purpose, 0.1% TiO2 nanoparticles less than 100 nm were mixed with human serum albumin (HSA), 0.1% and 1%, in cell culture medium (DMEM, pH 7.2). The Ti concentrations in the resulting solutions were analyzed by inductively coupled plasma mass spectrometry. The stability of the nanoparticles in suspension was analyzed by UV-vis spectrophotometer and Dynamic Light Scattering. The concentration of Ti in suspension was dependent on the presence and concentration of HSA. Albumin prevented high aggregation rate of TiO2 nanoparticles in cell culture medium. It is shown that nano TiO2-protein stable aggregates can be produced under physiological conditions at high concentrations, and are candidates for use in cellular tests.Keywords: titanium, nanoparticle, hypersensitivity to Ti Carmen Irina VamanuPaul Johan H&oslash;lZouhir EkelAllouniSaid ElsayedNils Roar GjerdetDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2008, Iss Issue 1, Pp 69-74 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Carmen Irina Vamanu Paul Johan H&oslash;l Zouhir Ekel Allouni Said Elsayed Nils Roar Gjerdet Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
description	Carmen Irina Vamanu1, Paul Johan H&oslash;l2, Zouhir Ekeland Allouni2, Said Elsayed3, Nils Roar Gjerdet11Departments of Oral Sciences &ndash; Dental Biomaterials, Faculty of Dentistry, University of Bergen, Bergen, Norway; 2Orthopedic Biomaterials, Helse Bergen Haukeland University Hospital, Bergen, Norway; 3Department of Medicine, University of Bergen, Bergen, NorwayAbstract: Degradation products of titanium implants include free ions, organo-metallic complexes, and particles, ranging from nano to macro sizes. The biological effects, especially of nanoparticles, is yet unknown. The main objective of this study was to develop Ti-protein antigens in physiological solutions that can be used in testing of cellular responses. For this purpose, 0.1% TiO2 nanoparticles less than 100 nm were mixed with human serum albumin (HSA), 0.1% and 1%, in cell culture medium (DMEM, pH 7.2). The Ti concentrations in the resulting solutions were analyzed by inductively coupled plasma mass spectrometry. The stability of the nanoparticles in suspension was analyzed by UV-vis spectrophotometer and Dynamic Light Scattering. The concentration of Ti in suspension was dependent on the presence and concentration of HSA. Albumin prevented high aggregation rate of TiO2 nanoparticles in cell culture medium. It is shown that nano TiO2-protein stable aggregates can be produced under physiological conditions at high concentrations, and are candidates for use in cellular tests.Keywords: titanium, nanoparticle, hypersensitivity to Ti
format	article
author	Carmen Irina Vamanu Paul Johan H&oslash;l Zouhir Ekel Allouni Said Elsayed Nils Roar Gjerdet
author_facet	Carmen Irina Vamanu Paul Johan H&oslash;l Zouhir Ekel Allouni Said Elsayed Nils Roar Gjerdet
author_sort	Carmen Irina Vamanu
title	Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_short	Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_full	Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_fullStr	Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_full_unstemmed	Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_sort	formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/b296e822f6074577a9d393351147e3de
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_version_	1718404008898461696

Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles

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