Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production

Abstract Microbial organelles are a promising model to promote cellular functions for the production of high-value chemicals. However, the concentrations of enzymes and nanoparticles are limited by the contact surface in single Escherichia coli cells. Herein, the definition of contact surface is to...

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Autores principales:	Qiang Ding, Yadi Liu, Guipeng Hu, Liang Guo, Cong Gao, Xiulai Chen, Wei Chen, Jian Chen, Liming Liu
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Biofilm Contact surface Self-assembly Biohybrid Shikimate L-malate Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b2ade4fe19474382b8c39d066fa2cb33
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spelling	oai:doaj.org-article:b2ade4fe19474382b8c39d066fa2cb332021-12-05T12:03:56ZEngineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production10.1186/s40643-021-00470-72197-4365https://doaj.org/article/b2ade4fe19474382b8c39d066fa2cb332021-11-01T00:00:00Zhttps://doi.org/10.1186/s40643-021-00470-7https://doaj.org/toc/2197-4365Abstract Microbial organelles are a promising model to promote cellular functions for the production of high-value chemicals. However, the concentrations of enzymes and nanoparticles are limited by the contact surface in single Escherichia coli cells. Herein, the definition of contact surface is to improve the amylase and CdS nanoparticles concentration for enhancing the substrate starch and cofactor NADH utilization. In this study, two biofilm-based strategies were developed to improve the contact surface for the production of shikimate and L-malate. First, the contact surface of E. coli was improved by amylase self-assembly with a blue light-inducible biofilm-based SpyTag/SpyCatcher system. This system increased the glucose concentration by 20.7% and the starch-based shikimate titer to 50.96 g L−1, which showed the highest titer with starch as substrate. Then, the contact surface of E. coli was improved using a biofilm-based CdS-biohybrid system by light-driven system, which improved the NADH concentration by 83.3% and increased the NADH-dependent L-malate titer to 45.93 g L−1. Thus, the biofilm-based strategies can regulate cellular functions to increase the efficiency of microbial cell factories based on the optogenetics, light-driven, and metabolic engineering. Graphical AbstractQiang DingYadi LiuGuipeng HuLiang GuoCong GaoXiulai ChenWei ChenJian ChenLiming LiuSpringerOpenarticleBiofilmContact surfaceSelf-assemblyBiohybridShikimateL-malateTechnologyTChemical technologyTP1-1185BiotechnologyTP248.13-248.65ENBioresources and Bioprocessing, Vol 8, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biofilm Contact surface Self-assembly Biohybrid Shikimate L-malate Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65
spellingShingle	Biofilm Contact surface Self-assembly Biohybrid Shikimate L-malate Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Qiang Ding Yadi Liu Guipeng Hu Liang Guo Cong Gao Xiulai Chen Wei Chen Jian Chen Liming Liu Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production
description	Abstract Microbial organelles are a promising model to promote cellular functions for the production of high-value chemicals. However, the concentrations of enzymes and nanoparticles are limited by the contact surface in single Escherichia coli cells. Herein, the definition of contact surface is to improve the amylase and CdS nanoparticles concentration for enhancing the substrate starch and cofactor NADH utilization. In this study, two biofilm-based strategies were developed to improve the contact surface for the production of shikimate and L-malate. First, the contact surface of E. coli was improved by amylase self-assembly with a blue light-inducible biofilm-based SpyTag/SpyCatcher system. This system increased the glucose concentration by 20.7% and the starch-based shikimate titer to 50.96 g L−1, which showed the highest titer with starch as substrate. Then, the contact surface of E. coli was improved using a biofilm-based CdS-biohybrid system by light-driven system, which improved the NADH concentration by 83.3% and increased the NADH-dependent L-malate titer to 45.93 g L−1. Thus, the biofilm-based strategies can regulate cellular functions to increase the efficiency of microbial cell factories based on the optogenetics, light-driven, and metabolic engineering. Graphical Abstract
format	article
author	Qiang Ding Yadi Liu Guipeng Hu Liang Guo Cong Gao Xiulai Chen Wei Chen Jian Chen Liming Liu
author_facet	Qiang Ding Yadi Liu Guipeng Hu Liang Guo Cong Gao Xiulai Chen Wei Chen Jian Chen Liming Liu
author_sort	Qiang Ding
title	Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production
title_short	Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production
title_full	Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production
title_fullStr	Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production
title_full_unstemmed	Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production
title_sort	engineering escherichia coli biofilm to increase contact surface for shikimate and l-malate production
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/b2ade4fe19474382b8c39d066fa2cb33
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Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production

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