3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds

Carla Cunha1,2, Silvia Panseri3,4, Omar Villa1,2, Diego Silva1,2, Fabrizio Gelain1,21Department of Biotechnology and Biosciences, University of Milano-Bicocca; 2Center for Nanomedicine and Tissue Engineering, CNTE &ndash; A.O. Ospedale Niguarda Ca&#39; Granda, Milan; 3Laboratory of B...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cunha C, Panseri S, Villa O, Silva D, Gelain F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b2b0b21ea9b94b2e8bffc4cc9053e319
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b2b0b21ea9b94b2e8bffc4cc9053e319
record_format	dspace
spelling	oai:doaj.org-article:b2b0b21ea9b94b2e8bffc4cc9053e3192021-12-02T01:19:14Z3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds1176-91141178-2013https://doaj.org/article/b2b0b21ea9b94b2e8bffc4cc9053e3192011-05-01T00:00:00Zhttp://www.dovepress.com/3d-culture-of-adult-mouse-neural-stem-cells-within-functionalized-self-a7313https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Carla Cunha1,2, Silvia Panseri3,4, Omar Villa1,2, Diego Silva1,2, Fabrizio Gelain1,21Department of Biotechnology and Biosciences, University of Milano-Bicocca; 2Center for Nanomedicine and Tissue Engineering, CNTE &ndash; A.O. Ospedale Niguarda Ca&#39; Granda, Milan; 3Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna; 4Laboratory of Nano-Biomagnetism, Institute of Science and Technology for Ceramics, National Research Council, Faenza, ItalyAbstract: Three-dimensional (3D) in vitro models of cell culture aim to fill the gap between the standard two-dimensional cell studies and the in vivo environment. Especially for neural tissue regeneration approaches where there is little regenerative capacity, these models are important for mimicking the extracellular matrix in providing support, allowing the natural flow of oxygen, nutrients, and growth factors, and possibly favoring neural cell regrowth. We have previously demonstrated that a new self-assembling nanostructured biomaterial, based on matrigel, was able to support adult neural stem cell (NSC) culture. In this study, we developed a new 3D cell culture system that takes advantage of the nano- and microfiber assembling process, under physiologic conditions, of these biomaterials. The assembled scaffold forms an intricate and biologically active matrix that displays specifically designed functional motifs: RGD (Arg-Gly-Asp), BMHP1 (bone marrow homing peptide 1), and BMHP2, for the culture of adult NSCs. These scaffolds were prepared at different concentrations, and microscopic examination of the cell-embedded scaffolds showed that NSCs are viable and they proliferate and differentiate within the nanostructured environment of the scaffold. Such a model has the potential to be tailored to develop ad hoc designed peptides for specific cell lines.Keywords: biomaterials, tissue engineering, 3D in vitro modelCunha CPanseri SVilla OSilva DGelain FDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 943-955 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cunha C Panseri S Villa O Silva D Gelain F 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
description	Carla Cunha1,2, Silvia Panseri3,4, Omar Villa1,2, Diego Silva1,2, Fabrizio Gelain1,21Department of Biotechnology and Biosciences, University of Milano-Bicocca; 2Center for Nanomedicine and Tissue Engineering, CNTE &ndash; A.O. Ospedale Niguarda Ca&#39; Granda, Milan; 3Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna; 4Laboratory of Nano-Biomagnetism, Institute of Science and Technology for Ceramics, National Research Council, Faenza, ItalyAbstract: Three-dimensional (3D) in vitro models of cell culture aim to fill the gap between the standard two-dimensional cell studies and the in vivo environment. Especially for neural tissue regeneration approaches where there is little regenerative capacity, these models are important for mimicking the extracellular matrix in providing support, allowing the natural flow of oxygen, nutrients, and growth factors, and possibly favoring neural cell regrowth. We have previously demonstrated that a new self-assembling nanostructured biomaterial, based on matrigel, was able to support adult neural stem cell (NSC) culture. In this study, we developed a new 3D cell culture system that takes advantage of the nano- and microfiber assembling process, under physiologic conditions, of these biomaterials. The assembled scaffold forms an intricate and biologically active matrix that displays specifically designed functional motifs: RGD (Arg-Gly-Asp), BMHP1 (bone marrow homing peptide 1), and BMHP2, for the culture of adult NSCs. These scaffolds were prepared at different concentrations, and microscopic examination of the cell-embedded scaffolds showed that NSCs are viable and they proliferate and differentiate within the nanostructured environment of the scaffold. Such a model has the potential to be tailored to develop ad hoc designed peptides for specific cell lines.Keywords: biomaterials, tissue engineering, 3D in vitro model
format	article
author	Cunha C Panseri S Villa O Silva D Gelain F
author_facet	Cunha C Panseri S Villa O Silva D Gelain F
author_sort	Cunha C
title	3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_short	3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_full	3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_fullStr	3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_full_unstemmed	3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
title_sort	3d culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/b2b0b21ea9b94b2e8bffc4cc9053e319
work_keys_str_mv	AT cunhac 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds AT panseris 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds AT villao 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds AT silvad 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds AT gelainf 3dcultureofadultmouseneuralstemcellswithinfunctionalizedselfassemblingpeptidescaffolds
_version_	1718403157854257152

3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds

Ejemplares similares