Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, respectively.

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Autores principales:	Kendall R. Sanson, Ruth E. Hanna, Mudra Hegde, Katherine F. Donovan, Christine Strand, Meagan E. Sullender, Emma W. Vaimberg, Amy Goodale, David E. Root, Federica Piccioni, John G. Doench
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b2b3b6edfdcb4b859c371ceb1069d5c6
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spelling	oai:doaj.org-article:b2b3b6edfdcb4b859c371ceb1069d5c62021-12-02T16:56:53ZOptimized libraries for CRISPR-Cas9 genetic screens with multiple modalities10.1038/s41467-018-07901-82041-1723https://doaj.org/article/b2b3b6edfdcb4b859c371ceb1069d5c62018-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07901-8https://doaj.org/toc/2041-1723Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, respectively.Kendall R. SansonRuth E. HannaMudra HegdeKatherine F. DonovanChristine StrandMeagan E. SullenderEmma W. VaimbergAmy GoodaleDavid E. RootFederica PiccioniJohn G. DoenchNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Kendall R. Sanson Ruth E. Hanna Mudra Hegde Katherine F. Donovan Christine Strand Meagan E. Sullender Emma W. Vaimberg Amy Goodale David E. Root Federica Piccioni John G. Doench Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
description	Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, respectively.
format	article
author	Kendall R. Sanson Ruth E. Hanna Mudra Hegde Katherine F. Donovan Christine Strand Meagan E. Sullender Emma W. Vaimberg Amy Goodale David E. Root Federica Piccioni John G. Doench
author_facet	Kendall R. Sanson Ruth E. Hanna Mudra Hegde Katherine F. Donovan Christine Strand Meagan E. Sullender Emma W. Vaimberg Amy Goodale David E. Root Federica Piccioni John G. Doench
author_sort	Kendall R. Sanson
title	Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
title_short	Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
title_full	Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
title_fullStr	Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
title_full_unstemmed	Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
title_sort	optimized libraries for crispr-cas9 genetic screens with multiple modalities
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b2b3b6edfdcb4b859c371ceb1069d5c6
work_keys_str_mv	AT kendallrsanson optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT ruthehanna optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT mudrahegde optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT katherinefdonovan optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT christinestrand optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT meaganesullender optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT emmawvaimberg optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT amygoodale optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT davideroot optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT federicapiccioni optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities AT johngdoench optimizedlibrariesforcrisprcas9geneticscreenswithmultiplemodalities
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Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

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