Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices

Abstract Nanoplastic particulates (pNP) are widely considered as being potentially harmful to the environment and living organisms while also being technically difficult to detect and identify in the presence of biological matrices. In this study, we describe a method for the extraction and subseque...

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Main Authors:	Andrea Valsesia, Monica Quarato, Jessica Ponti, Francesco Fumagalli, Douglas Gilliland, Pascal Colpo
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/b2b4dd18238f41a39da3df7b0effa9bc
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spelling	oai:doaj.org-article:b2b4dd18238f41a39da3df7b0effa9bc2021-12-02T15:22:58ZCombining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices10.1038/s41598-020-79714-z2045-2322https://doaj.org/article/b2b4dd18238f41a39da3df7b0effa9bc2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79714-zhttps://doaj.org/toc/2045-2322Abstract Nanoplastic particulates (pNP) are widely considered as being potentially harmful to the environment and living organisms while also being technically difficult to detect and identify in the presence of biological matrices. In this study, we describe a method for the extraction and subsequent Raman analysis of pNP present in the tissues of salt-water mussels. The process combines a step of enzymatic digestion/filtering to eliminate the biological matrix with a detection/identification procedure, which uses a micro-machined surface, composed of arrays of cavities with well-defined sub-micron depths and diameters. This sensor surface, exploits capillary forces in a drying droplet of analyte solution to drive the self-assembly of suspended nanoparticles into the cavities leaving the individual particles isolated from each other over the surface. The resulting array, when analysed using confocal Raman microscopy, permits the size selective analysis of the individual sub-micron pNP trapped in the cavities structure.Andrea ValsesiaMonica QuaratoJessica PontiFrancesco FumagalliDouglas GillilandPascal ColpoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrea Valsesia Monica Quarato Jessica Ponti Francesco Fumagalli Douglas Gilliland Pascal Colpo Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices
description	Abstract Nanoplastic particulates (pNP) are widely considered as being potentially harmful to the environment and living organisms while also being technically difficult to detect and identify in the presence of biological matrices. In this study, we describe a method for the extraction and subsequent Raman analysis of pNP present in the tissues of salt-water mussels. The process combines a step of enzymatic digestion/filtering to eliminate the biological matrix with a detection/identification procedure, which uses a micro-machined surface, composed of arrays of cavities with well-defined sub-micron depths and diameters. This sensor surface, exploits capillary forces in a drying droplet of analyte solution to drive the self-assembly of suspended nanoparticles into the cavities leaving the individual particles isolated from each other over the surface. The resulting array, when analysed using confocal Raman microscopy, permits the size selective analysis of the individual sub-micron pNP trapped in the cavities structure.
format	article
author	Andrea Valsesia Monica Quarato Jessica Ponti Francesco Fumagalli Douglas Gilliland Pascal Colpo
author_facet	Andrea Valsesia Monica Quarato Jessica Ponti Francesco Fumagalli Douglas Gilliland Pascal Colpo
author_sort	Andrea Valsesia
title	Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices
title_short	Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices
title_full	Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices
title_fullStr	Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices
title_full_unstemmed	Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices
title_sort	combining microcavity size selection with raman microscopy for the characterization of nanoplastics in complex matrices
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b2b4dd18238f41a39da3df7b0effa9bc
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Combining microcavity size selection with Raman microscopy for the characterization of Nanoplastics in complex matrices

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