Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.

Age-related changes in various tissues have been associated with the onset of a number of age-related diseases, including inflammation and cancer. Bladder cancer, for instance, is a disease that mainly afflicts middle-aged or elderly people and is mostly of urothelial origin. Although research on ag...

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Autores principales:	Martina Perše, Rade Injac, Andreja Erman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b2c29c972ce74d2098d2b717f816fbe6
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spelling	oai:doaj.org-article:b2c29c972ce74d2098d2b717f816fbe62021-11-18T07:52:38ZOxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.1932-620310.1371/journal.pone.0059638https://doaj.org/article/b2c29c972ce74d2098d2b717f816fbe62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23527235/?tool=EBIhttps://doaj.org/toc/1932-6203Age-related changes in various tissues have been associated with the onset of a number of age-related diseases, including inflammation and cancer. Bladder cancer, for instance, is a disease that mainly afflicts middle-aged or elderly people and is mostly of urothelial origin. Although research on age-related changes of long-lived post-mitotic cells such as neurons is rapidly progressing, nothing is known about age-related changes in the urothelium of the urinary bladder, despite all the evidence confirming the important role of oxidative stress in urinary bladder pathology. The purpose of this study was thus to investigate the oxidative status and age-related changes in urothelial cells of the urinary bladder of young (2 months) and aging (20 months) mice by means of various methods. Our results demonstrated that healthy young urothelium possesses a powerful antioxidant defence system that functions as a strong defence barrier against reactive species. In contrast, urothelial cells of aging bladder show significantly decreased total antioxidant capacity and significantly increased levels of lipid peroxides (MDA) and iNOS, markers of oxidative stress. Our study demonstrates for the first time that ultrastructural alterations in mitochondria and accumulation of lipofuscin, known to be one of the aging pigments, can clearly be found in superficial urothelial cells of the urinary bladder in aging mice. Since the presence of lipofuscin in the urothelium has not yet been reported, we applied various methods to confirm our finding. Our results reveal changes in the oxidative status and structural alterations to superficial urothelial cells similar to those of other long-lived post-mitotic cells.Martina PeršeRade InjacAndreja ErmanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59638 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martina Perše Rade Injac Andreja Erman Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.
description	Age-related changes in various tissues have been associated with the onset of a number of age-related diseases, including inflammation and cancer. Bladder cancer, for instance, is a disease that mainly afflicts middle-aged or elderly people and is mostly of urothelial origin. Although research on age-related changes of long-lived post-mitotic cells such as neurons is rapidly progressing, nothing is known about age-related changes in the urothelium of the urinary bladder, despite all the evidence confirming the important role of oxidative stress in urinary bladder pathology. The purpose of this study was thus to investigate the oxidative status and age-related changes in urothelial cells of the urinary bladder of young (2 months) and aging (20 months) mice by means of various methods. Our results demonstrated that healthy young urothelium possesses a powerful antioxidant defence system that functions as a strong defence barrier against reactive species. In contrast, urothelial cells of aging bladder show significantly decreased total antioxidant capacity and significantly increased levels of lipid peroxides (MDA) and iNOS, markers of oxidative stress. Our study demonstrates for the first time that ultrastructural alterations in mitochondria and accumulation of lipofuscin, known to be one of the aging pigments, can clearly be found in superficial urothelial cells of the urinary bladder in aging mice. Since the presence of lipofuscin in the urothelium has not yet been reported, we applied various methods to confirm our finding. Our results reveal changes in the oxidative status and structural alterations to superficial urothelial cells similar to those of other long-lived post-mitotic cells.
format	article
author	Martina Perše Rade Injac Andreja Erman
author_facet	Martina Perše Rade Injac Andreja Erman
author_sort	Martina Perše
title	Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.
title_short	Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.
title_full	Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.
title_fullStr	Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.
title_full_unstemmed	Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.
title_sort	oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b2c29c972ce74d2098d2b717f816fbe6
work_keys_str_mv	AT martinaperse oxidativestatusandlipofuscinaccumulationinurothelialcellsofbladderinagingmice AT radeinjac oxidativestatusandlipofuscinaccumulationinurothelialcellsofbladderinagingmice AT andrejaerman oxidativestatusandlipofuscinaccumulationinurothelialcellsofbladderinagingmice
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Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice.

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