The evolution of multiple active site configurations in a designed enzyme

Generation and iterative optimization of designed enzymes can provide valuable insights for a more efficient catalysis. Here the authors have followed the iterative improvement of a designed Kemp eliminase and show that remote point mutations could remodel the designed active site via substantial co...

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Autores principales:	Nan-Sook Hong, Dušan Petrović, Richmond Lee, Ganna Gryn’ova, Miha Purg, Jake Saunders, Paul Bauer, Paul D. Carr, Ching-Yeh Lin, Peter D. Mabbitt, William Zhang, Timothy Altamore, Chris Easton, Michelle L. Coote, Shina C. L. Kamerlin, Colin J. Jackson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b2c884a3737b4e24b72bcbda3206e292
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spelling	oai:doaj.org-article:b2c884a3737b4e24b72bcbda3206e2922021-12-02T14:39:44ZThe evolution of multiple active site configurations in a designed enzyme10.1038/s41467-018-06305-y2041-1723https://doaj.org/article/b2c884a3737b4e24b72bcbda3206e2922018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06305-yhttps://doaj.org/toc/2041-1723Generation and iterative optimization of designed enzymes can provide valuable insights for a more efficient catalysis. Here the authors have followed the iterative improvement of a designed Kemp eliminase and show that remote point mutations could remodel the designed active site via substantial conformational reorganization.Nan-Sook HongDušan PetrovićRichmond LeeGanna Gryn’ovaMiha PurgJake SaundersPaul BauerPaul D. CarrChing-Yeh LinPeter D. MabbittWilliam ZhangTimothy AltamoreChris EastonMichelle L. CooteShina C. L. KamerlinColin J. JacksonNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nan-Sook Hong Dušan Petrović Richmond Lee Ganna Gryn’ova Miha Purg Jake Saunders Paul Bauer Paul D. Carr Ching-Yeh Lin Peter D. Mabbitt William Zhang Timothy Altamore Chris Easton Michelle L. Coote Shina C. L. Kamerlin Colin J. Jackson The evolution of multiple active site configurations in a designed enzyme
description	Generation and iterative optimization of designed enzymes can provide valuable insights for a more efficient catalysis. Here the authors have followed the iterative improvement of a designed Kemp eliminase and show that remote point mutations could remodel the designed active site via substantial conformational reorganization.
format	article
author	Nan-Sook Hong Dušan Petrović Richmond Lee Ganna Gryn’ova Miha Purg Jake Saunders Paul Bauer Paul D. Carr Ching-Yeh Lin Peter D. Mabbitt William Zhang Timothy Altamore Chris Easton Michelle L. Coote Shina C. L. Kamerlin Colin J. Jackson
author_facet	Nan-Sook Hong Dušan Petrović Richmond Lee Ganna Gryn’ova Miha Purg Jake Saunders Paul Bauer Paul D. Carr Ching-Yeh Lin Peter D. Mabbitt William Zhang Timothy Altamore Chris Easton Michelle L. Coote Shina C. L. Kamerlin Colin J. Jackson
author_sort	Nan-Sook Hong
title	The evolution of multiple active site configurations in a designed enzyme
title_short	The evolution of multiple active site configurations in a designed enzyme
title_full	The evolution of multiple active site configurations in a designed enzyme
title_fullStr	The evolution of multiple active site configurations in a designed enzyme
title_full_unstemmed	The evolution of multiple active site configurations in a designed enzyme
title_sort	evolution of multiple active site configurations in a designed enzyme
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b2c884a3737b4e24b72bcbda3206e292
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The evolution of multiple active site configurations in a designed enzyme

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