Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing

Abstract Background Urine from clinically healthy dogs is not sterile. Characterizing microbial diversity and abundance within this population of dogs is important to define normal reference ranges for healthy urine. Objectives To establish composition and relative representation of bacterial and fu...

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Autores principales:	Tonatiuh Melgarejo, Brian B. Oakley, Janina A. Krumbeck, Shuiquan Tang, Adam Krantz, Annika Linde
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	culture‐independent microbiome microbiota mycobiome urinalysis Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/b2d912c305e64532b6031c966b17ec11
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spelling	oai:doaj.org-article:b2d912c305e64532b6031c966b17ec112021-11-30T17:01:04ZAssessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing1939-16760891-664010.1111/jvim.16104https://doaj.org/article/b2d912c305e64532b6031c966b17ec112021-05-01T00:00:00Zhttps://doi.org/10.1111/jvim.16104https://doaj.org/toc/0891-6640https://doaj.org/toc/1939-1676Abstract Background Urine from clinically healthy dogs is not sterile. Characterizing microbial diversity and abundance within this population of dogs is important to define normal reference ranges for healthy urine. Objectives To establish composition and relative representation of bacterial and fungal microbiomes in urine of clinically healthy dogs. Animals Fifty clinically healthy dogs. Methods Analytic study. Urine sampling via cystocentesis. Comprehensive evaluation of urine including standard urinalysis, culture and sensitivity, next‐generation sequencing (NGS), and bioinformatics to define bacterial and fungal microbiome. Results Culture did not yield positive results in any samples. Next‐generation sequencing of urine established low presence of bacteria, fungi, or both in all samples. Diversity and abundance of bacterial and fungal communities varied between urine samples from different dogs. Struvite crystals were associated with bacterial community structure (P = .07) and there was a positive correlation between struvite crystals and pH. Conclusions and Clinical Importance The microbiome in urine of clinically healthy dogs has diverse bacterial and fungal species These findings highlight limitations of conventional culture testing and the need for culture‐independent molecular diagnostics to detect microorganisms in urine.Tonatiuh MelgarejoBrian B. OakleyJanina A. KrumbeckShuiquan TangAdam KrantzAnnika LindeWileyarticleculture‐independentmicrobiomemicrobiotamycobiomeurinalysisVeterinary medicineSF600-1100ENJournal of Veterinary Internal Medicine, Vol 35, Iss 3, Pp 1416-1426 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	culture‐independent microbiome microbiota mycobiome urinalysis Veterinary medicine SF600-1100
spellingShingle	culture‐independent microbiome microbiota mycobiome urinalysis Veterinary medicine SF600-1100 Tonatiuh Melgarejo Brian B. Oakley Janina A. Krumbeck Shuiquan Tang Adam Krantz Annika Linde Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing
description	Abstract Background Urine from clinically healthy dogs is not sterile. Characterizing microbial diversity and abundance within this population of dogs is important to define normal reference ranges for healthy urine. Objectives To establish composition and relative representation of bacterial and fungal microbiomes in urine of clinically healthy dogs. Animals Fifty clinically healthy dogs. Methods Analytic study. Urine sampling via cystocentesis. Comprehensive evaluation of urine including standard urinalysis, culture and sensitivity, next‐generation sequencing (NGS), and bioinformatics to define bacterial and fungal microbiome. Results Culture did not yield positive results in any samples. Next‐generation sequencing of urine established low presence of bacteria, fungi, or both in all samples. Diversity and abundance of bacterial and fungal communities varied between urine samples from different dogs. Struvite crystals were associated with bacterial community structure (P = .07) and there was a positive correlation between struvite crystals and pH. Conclusions and Clinical Importance The microbiome in urine of clinically healthy dogs has diverse bacterial and fungal species These findings highlight limitations of conventional culture testing and the need for culture‐independent molecular diagnostics to detect microorganisms in urine.
format	article
author	Tonatiuh Melgarejo Brian B. Oakley Janina A. Krumbeck Shuiquan Tang Adam Krantz Annika Linde
author_facet	Tonatiuh Melgarejo Brian B. Oakley Janina A. Krumbeck Shuiquan Tang Adam Krantz Annika Linde
author_sort	Tonatiuh Melgarejo
title	Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing
title_short	Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing
title_full	Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing
title_fullStr	Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing
title_full_unstemmed	Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing
title_sort	assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/b2d912c305e64532b6031c966b17ec11
work_keys_str_mv	AT tonatiuhmelgarejo assessmentofbacterialandfungalpopulationsinurinefromclinicallyhealthydogsusingnextgenerationsequencing AT brianboakley assessmentofbacterialandfungalpopulationsinurinefromclinicallyhealthydogsusingnextgenerationsequencing AT janinaakrumbeck assessmentofbacterialandfungalpopulationsinurinefromclinicallyhealthydogsusingnextgenerationsequencing AT shuiquantang assessmentofbacterialandfungalpopulationsinurinefromclinicallyhealthydogsusingnextgenerationsequencing AT adamkrantz assessmentofbacterialandfungalpopulationsinurinefromclinicallyhealthydogsusingnextgenerationsequencing AT annikalinde assessmentofbacterialandfungalpopulationsinurinefromclinicallyhealthydogsusingnextgenerationsequencing
_version_	1718406467045818368

Assessment of bacterial and fungal populations in urine from clinically healthy dogs using next‐generation sequencing

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