Interactions between gastric microbiota and metabolites in gastric cancer

Abstract The development and progression of gastric cancer (GC) is greatly influenced by gastric microbiota and their metabolites. Here, we characterized the gastric microbiome and metabolome profiles of 37 GC tumor tissues and matched non-tumor tissues using 16s rRNA gene sequencing and ultrahigh p...

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Autores principales:	Daofeng Dai, Yan Yang, Jieqing Yu, Tianfeng Dang, Wenjing Qin, Lisong Teng, Jing Ye, Hongqun Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/b2e1d0b747d24b8a9d0e8536082b18cd
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spelling	oai:doaj.org-article:b2e1d0b747d24b8a9d0e8536082b18cd2021-11-28T12:04:27ZInteractions between gastric microbiota and metabolites in gastric cancer10.1038/s41419-021-04396-y2041-4889https://doaj.org/article/b2e1d0b747d24b8a9d0e8536082b18cd2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41419-021-04396-yhttps://doaj.org/toc/2041-4889Abstract The development and progression of gastric cancer (GC) is greatly influenced by gastric microbiota and their metabolites. Here, we characterized the gastric microbiome and metabolome profiles of 37 GC tumor tissues and matched non-tumor tissues using 16s rRNA gene sequencing and ultrahigh performance liquid chromatography tandem mass spectrometry, respectively. Microbial diversity and richness were higher in GC tumor tissues than in non-tumor tissues. The abundance of Helicobacter was increased in non-tumor tissues, while the abundance of Lactobacillus, Streptococcus, Bacteroides, Prevotella, and 6 additional genera was increased in the tumor tissues. The untargeted metabolome analysis revealed 150 discriminative metabolites, among which the relative abundance of the amino acids, carbohydrates and carbohydrate conjugates, glycerophospholipids, and nucleosides was higher in tumor tissues compared to non-tumor tissues. The targeted metabolome analysis further demonstrated that the combination of 1-methylnicotinamide and N-acetyl-D-glucosamine-6-phosphate could serve as a robust biomarker for distinction between GC tumors and non-tumor tissues. Correlation analysis revealed that Helicobacter and Lactobacillus were negatively and positively correlated with the majority of differential metabolites in the classes of amino acids, carbohydrates, nucleosides, nucleotides, and glycerophospholipids, respectively, suggesting that Helicobacter and Lactobacillus might play a role in degradation and synthesis of the majority of differential metabolites in these classes, respectively. Acinetobacter, Comamonas, Faecalibacterium, Sphingomonas, and Streptococcus were also significantly correlated with many differential amino acids, carbohydrates, nucleosides, nucleotides, and glycerophospholipids. In conclusion, the differences in metabolome profiles between GC tumor and matched non-tumor tissues may be partly due to the collective activities of Helicobacter, Lactobacillus, and other bacteria, which eventually affects GC carcinogenesis and progression.Daofeng DaiYan YangJieqing YuTianfeng DangWenjing QinLisong TengJing YeHongqun JiangNature Publishing GrouparticleCytologyQH573-671ENCell Death and Disease, Vol 12, Iss 12, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Daofeng Dai Yan Yang Jieqing Yu Tianfeng Dang Wenjing Qin Lisong Teng Jing Ye Hongqun Jiang Interactions between gastric microbiota and metabolites in gastric cancer
description	Abstract The development and progression of gastric cancer (GC) is greatly influenced by gastric microbiota and their metabolites. Here, we characterized the gastric microbiome and metabolome profiles of 37 GC tumor tissues and matched non-tumor tissues using 16s rRNA gene sequencing and ultrahigh performance liquid chromatography tandem mass spectrometry, respectively. Microbial diversity and richness were higher in GC tumor tissues than in non-tumor tissues. The abundance of Helicobacter was increased in non-tumor tissues, while the abundance of Lactobacillus, Streptococcus, Bacteroides, Prevotella, and 6 additional genera was increased in the tumor tissues. The untargeted metabolome analysis revealed 150 discriminative metabolites, among which the relative abundance of the amino acids, carbohydrates and carbohydrate conjugates, glycerophospholipids, and nucleosides was higher in tumor tissues compared to non-tumor tissues. The targeted metabolome analysis further demonstrated that the combination of 1-methylnicotinamide and N-acetyl-D-glucosamine-6-phosphate could serve as a robust biomarker for distinction between GC tumors and non-tumor tissues. Correlation analysis revealed that Helicobacter and Lactobacillus were negatively and positively correlated with the majority of differential metabolites in the classes of amino acids, carbohydrates, nucleosides, nucleotides, and glycerophospholipids, respectively, suggesting that Helicobacter and Lactobacillus might play a role in degradation and synthesis of the majority of differential metabolites in these classes, respectively. Acinetobacter, Comamonas, Faecalibacterium, Sphingomonas, and Streptococcus were also significantly correlated with many differential amino acids, carbohydrates, nucleosides, nucleotides, and glycerophospholipids. In conclusion, the differences in metabolome profiles between GC tumor and matched non-tumor tissues may be partly due to the collective activities of Helicobacter, Lactobacillus, and other bacteria, which eventually affects GC carcinogenesis and progression.
format	article
author	Daofeng Dai Yan Yang Jieqing Yu Tianfeng Dang Wenjing Qin Lisong Teng Jing Ye Hongqun Jiang
author_facet	Daofeng Dai Yan Yang Jieqing Yu Tianfeng Dang Wenjing Qin Lisong Teng Jing Ye Hongqun Jiang
author_sort	Daofeng Dai
title	Interactions between gastric microbiota and metabolites in gastric cancer
title_short	Interactions between gastric microbiota and metabolites in gastric cancer
title_full	Interactions between gastric microbiota and metabolites in gastric cancer
title_fullStr	Interactions between gastric microbiota and metabolites in gastric cancer
title_full_unstemmed	Interactions between gastric microbiota and metabolites in gastric cancer
title_sort	interactions between gastric microbiota and metabolites in gastric cancer
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/b2e1d0b747d24b8a9d0e8536082b18cd
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_version_	1718408220553248768

Interactions between gastric microbiota and metabolites in gastric cancer

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