Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats

Wei Li,1 Kaizheng Gong,1 Yuan Ding,2 Birenda Chaurasiya,2 Yue Ni,1 Yong Wu,1 Pei Zhao,1 Yan Shen,2 Zhengang Zhang,1 Thomas J Webster3 1Department of Cardiology, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225000, China; 2Department of Pharmaceutics, School of Pharmacy, China Pharm...

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Autores principales:	Li W, Gong K, Ding Y, Chaurasiya B, Ni Y, Wu Y, Zhao P, Shen Y, Zhang Z, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Heart failure Nanosuspension Dilated cardiomyopathy Autoimmune myocarditis Triptolide Methotrexate Immunoregulation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b2eaa65a116b46b9b3dfff37aa7a0164
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spelling	oai:doaj.org-article:b2eaa65a116b46b9b3dfff37aa7a01642021-12-02T02:32:48ZEffects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats1178-2013https://doaj.org/article/b2eaa65a116b46b9b3dfff37aa7a01642019-01-01T00:00:00Zhttps://www.dovepress.com/effects-of-triptolide-and-methotrexate-nanosuspensions-on-left-ventric-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Li,1 Kaizheng Gong,1 Yuan Ding,2 Birenda Chaurasiya,2 Yue Ni,1 Yong Wu,1 Pei Zhao,1 Yan Shen,2 Zhengang Zhang,1 Thomas J Webster3 1Department of Cardiology, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225000, China; 2Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, China; 3Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA Purpose: This study was carried out to investigate the effects of a triptolide (TP) nanosuspension and methotrexate (MTX) nanosuspension on left ventricular remodeling and cardiac function for autoimmune myocarditis (EAM) in rats. The regulating effects on inflammatory cytokines in the peripheral serum and related mechanisms are also discussed. Methods: First, TP and MTX were prepared as a nanosuspension, and the EAM model was successfully established in rats with cardiac myosin. Then, the effect of TP and MTX suspensions was tested in an EAM model. Results: Results revealed that both TP and MTX suspensions could reduce the degree of myocardial fibrosis and delay the remodeling process of the left ventricle which could further improve cardiac function. Finally, it was found that inflammatory cytokines in the peripheral serum were regulated by the nonspecific immune system and the inhibition of nuclear factor-κB signaling might have partly occurred due to this mechanism. Conclusion: In summary, this study provided a complete foundation for EAM therapy of profound clinical relevance. Keywords: inflammatory cytokines, heart failure, dilated cardiomyopathy, immunoregulation, suspensionLi WGong KDing YChaurasiya BNi YWu YZhao PShen YZhang ZWebster TJDove Medical PressarticleHeart failureNanosuspensionDilated cardiomyopathyAutoimmune myocarditisTriptolideMethotrexateImmunoregulationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 851-863 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Heart failure Nanosuspension Dilated cardiomyopathy Autoimmune myocarditis Triptolide Methotrexate Immunoregulation Medicine (General) R5-920
spellingShingle	Heart failure Nanosuspension Dilated cardiomyopathy Autoimmune myocarditis Triptolide Methotrexate Immunoregulation Medicine (General) R5-920 Li W Gong K Ding Y Chaurasiya B Ni Y Wu Y Zhao P Shen Y Zhang Z Webster TJ Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats
description	Wei Li,1 Kaizheng Gong,1 Yuan Ding,2 Birenda Chaurasiya,2 Yue Ni,1 Yong Wu,1 Pei Zhao,1 Yan Shen,2 Zhengang Zhang,1 Thomas J Webster3 1Department of Cardiology, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225000, China; 2Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, China; 3Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA Purpose: This study was carried out to investigate the effects of a triptolide (TP) nanosuspension and methotrexate (MTX) nanosuspension on left ventricular remodeling and cardiac function for autoimmune myocarditis (EAM) in rats. The regulating effects on inflammatory cytokines in the peripheral serum and related mechanisms are also discussed. Methods: First, TP and MTX were prepared as a nanosuspension, and the EAM model was successfully established in rats with cardiac myosin. Then, the effect of TP and MTX suspensions was tested in an EAM model. Results: Results revealed that both TP and MTX suspensions could reduce the degree of myocardial fibrosis and delay the remodeling process of the left ventricle which could further improve cardiac function. Finally, it was found that inflammatory cytokines in the peripheral serum were regulated by the nonspecific immune system and the inhibition of nuclear factor-κB signaling might have partly occurred due to this mechanism. Conclusion: In summary, this study provided a complete foundation for EAM therapy of profound clinical relevance. Keywords: inflammatory cytokines, heart failure, dilated cardiomyopathy, immunoregulation, suspension
format	article
author	Li W Gong K Ding Y Chaurasiya B Ni Y Wu Y Zhao P Shen Y Zhang Z Webster TJ
author_facet	Li W Gong K Ding Y Chaurasiya B Ni Y Wu Y Zhao P Shen Y Zhang Z Webster TJ
author_sort	Li W
title	Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats
title_short	Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats
title_full	Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats
title_fullStr	Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats
title_full_unstemmed	Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats
title_sort	effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/b2eaa65a116b46b9b3dfff37aa7a0164
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Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats

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