Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense

Abstract The plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the...

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Autores principales:	José Francisco Cruz-Pérez, Roxana Lara-Oueilhe, Cynthia Marcos-Jiménez, Ricardo Cuatlayotl-Olarte, María Luisa Xiqui-Vázquez, Sandra Raquel Reyes-Carmona, Beatriz Eugenia Baca, Alberto Ramírez-Mata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3032d74a2f64e419f9323a53cd9b894
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spelling	oai:doaj.org-article:b3032d74a2f64e419f9323a53cd9b8942021-12-02T14:12:09ZExpression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense10.1038/s41598-020-80125-32045-2322https://doaj.org/article/b3032d74a2f64e419f9323a53cd9b8942021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80125-3https://doaj.org/toc/2045-2322Abstract The plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function and expression of the cdgD gene, encoding a multidomain protein that includes GGDEF-EAL domains and CHASE and PAS domains. An insertional cdgD gene mutant was constructed, and analysis of biofilm and extracellular polymeric substance production, as well as the motility phenotype indicated that cdgD encoded a functional diguanylate protein. These results were correlated with a reduced overall cellular concentration of cyclic-di-GMP in the mutant over 48 h compared with that observed in the wild-type strain, which was recovered in the complemented strain. In addition, cdgD gene expression was measured in cells growing under planktonic or biofilm conditions, and differential expression was observed when KNO3 or NH4Cl was added to the minimal medium as a nitrogen source. The transcriptional fusion of the cdgD promoter with the gene encoding the autofluorescent mCherry protein indicated that the cdgD gene was expressed both under abiotic conditions and in association with wheat roots. Reduced colonization of wheat roots was observed for the mutant compared with the wild-type strain grown in the same soil conditions. The Azospirillum-plant association begins with the motility of the bacterium towards the plant rhizosphere followed by the adsorption and adherence of these bacteria to plant roots. Therefore, it is important to study the genes that contribute to this initial interaction of the bacterium with its host plant.José Francisco Cruz-PérezRoxana Lara-OueilheCynthia Marcos-JiménezRicardo Cuatlayotl-OlarteMaría Luisa Xiqui-VázquezSandra Raquel Reyes-CarmonaBeatriz Eugenia BacaAlberto Ramírez-MataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q José Francisco Cruz-Pérez Roxana Lara-Oueilhe Cynthia Marcos-Jiménez Ricardo Cuatlayotl-Olarte María Luisa Xiqui-Vázquez Sandra Raquel Reyes-Carmona Beatriz Eugenia Baca Alberto Ramírez-Mata Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
description	Abstract The plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function and expression of the cdgD gene, encoding a multidomain protein that includes GGDEF-EAL domains and CHASE and PAS domains. An insertional cdgD gene mutant was constructed, and analysis of biofilm and extracellular polymeric substance production, as well as the motility phenotype indicated that cdgD encoded a functional diguanylate protein. These results were correlated with a reduced overall cellular concentration of cyclic-di-GMP in the mutant over 48 h compared with that observed in the wild-type strain, which was recovered in the complemented strain. In addition, cdgD gene expression was measured in cells growing under planktonic or biofilm conditions, and differential expression was observed when KNO3 or NH4Cl was added to the minimal medium as a nitrogen source. The transcriptional fusion of the cdgD promoter with the gene encoding the autofluorescent mCherry protein indicated that the cdgD gene was expressed both under abiotic conditions and in association with wheat roots. Reduced colonization of wheat roots was observed for the mutant compared with the wild-type strain grown in the same soil conditions. The Azospirillum-plant association begins with the motility of the bacterium towards the plant rhizosphere followed by the adsorption and adherence of these bacteria to plant roots. Therefore, it is important to study the genes that contribute to this initial interaction of the bacterium with its host plant.
format	article
author	José Francisco Cruz-Pérez Roxana Lara-Oueilhe Cynthia Marcos-Jiménez Ricardo Cuatlayotl-Olarte María Luisa Xiqui-Vázquez Sandra Raquel Reyes-Carmona Beatriz Eugenia Baca Alberto Ramírez-Mata
author_facet	José Francisco Cruz-Pérez Roxana Lara-Oueilhe Cynthia Marcos-Jiménez Ricardo Cuatlayotl-Olarte María Luisa Xiqui-Vázquez Sandra Raquel Reyes-Carmona Beatriz Eugenia Baca Alberto Ramírez-Mata
author_sort	José Francisco Cruz-Pérez
title	Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_short	Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_full	Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_fullStr	Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_full_unstemmed	Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense
title_sort	expression and function of the cdgd gene, encoding a chase–pas-dgc-eal domain protein, in azospirillum brasilense
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b3032d74a2f64e419f9323a53cd9b894
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Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense

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