Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets

Abstract Influenza viruses cause annual seasonal epidemics and sporadic pandemics; vaccination is the most effective countermeasure. Intranasal live attenuated influenza vaccines (LAIVs) are needle-free, mimic the natural route of infection, and elicit robust immunity. However, some LAIVs require re...

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Autores principales:	Jasmina M. Luczo, Tatiana Bousse, Scott K. Johnson, Cheryl A. Jones, Nicholas Pearce, Carlie A. Neiswanger, Min-Xuan Wang, Erin A. Miller, Nikolai Petrovsky, David E. Wentworth, Victor Bronshtein, Mark Papania, Stephen M. Tompkins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/b30473452fc54337aa97734a70ab5f16
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spelling	oai:doaj.org-article:b30473452fc54337aa97734a70ab5f162021-12-02T13:39:34ZIntranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets10.1038/s41541-021-00320-92059-0105https://doaj.org/article/b30473452fc54337aa97734a70ab5f162021-04-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00320-9https://doaj.org/toc/2059-0105Abstract Influenza viruses cause annual seasonal epidemics and sporadic pandemics; vaccination is the most effective countermeasure. Intranasal live attenuated influenza vaccines (LAIVs) are needle-free, mimic the natural route of infection, and elicit robust immunity. However, some LAIVs require reconstitution and cold-chain requirements restrict storage and distribution of all influenza vaccines. We generated a dry-powder, thermostable LAIV (T-LAIV) using Preservation by Vaporization technology and assessed the stability, immunogenicity, and efficacy of T-LAIV alone or combined with delta inulin adjuvant (Advax™) in ferrets. Stability assays demonstrated minimal loss of T-LAIV titer when stored at 25 °C for 1 year. Vaccination of ferrets with T-LAIV alone or with delta inulin adjuvant elicited mucosal antibody and robust serum HI responses in ferrets, and was protective against homologous challenge. These results suggest that the Preservation by Vaporization-generated dry-powder vaccines could be distributed without refrigeration and administered without reconstitution or injection. Given these significant advantages for vaccine distribution and delivery, further research is warranted.Jasmina M. LuczoTatiana BousseScott K. JohnsonCheryl A. JonesNicholas PearceCarlie A. NeiswangerMin-Xuan WangErin A. MillerNikolai PetrovskyDavid E. WentworthVictor BronshteinMark PapaniaStephen M. TompkinsNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Jasmina M. Luczo Tatiana Bousse Scott K. Johnson Cheryl A. Jones Nicholas Pearce Carlie A. Neiswanger Min-Xuan Wang Erin A. Miller Nikolai Petrovsky David E. Wentworth Victor Bronshtein Mark Papania Stephen M. Tompkins Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
description	Abstract Influenza viruses cause annual seasonal epidemics and sporadic pandemics; vaccination is the most effective countermeasure. Intranasal live attenuated influenza vaccines (LAIVs) are needle-free, mimic the natural route of infection, and elicit robust immunity. However, some LAIVs require reconstitution and cold-chain requirements restrict storage and distribution of all influenza vaccines. We generated a dry-powder, thermostable LAIV (T-LAIV) using Preservation by Vaporization technology and assessed the stability, immunogenicity, and efficacy of T-LAIV alone or combined with delta inulin adjuvant (Advax™) in ferrets. Stability assays demonstrated minimal loss of T-LAIV titer when stored at 25 °C for 1 year. Vaccination of ferrets with T-LAIV alone or with delta inulin adjuvant elicited mucosal antibody and robust serum HI responses in ferrets, and was protective against homologous challenge. These results suggest that the Preservation by Vaporization-generated dry-powder vaccines could be distributed without refrigeration and administered without reconstitution or injection. Given these significant advantages for vaccine distribution and delivery, further research is warranted.
format	article
author	Jasmina M. Luczo Tatiana Bousse Scott K. Johnson Cheryl A. Jones Nicholas Pearce Carlie A. Neiswanger Min-Xuan Wang Erin A. Miller Nikolai Petrovsky David E. Wentworth Victor Bronshtein Mark Papania Stephen M. Tompkins
author_facet	Jasmina M. Luczo Tatiana Bousse Scott K. Johnson Cheryl A. Jones Nicholas Pearce Carlie A. Neiswanger Min-Xuan Wang Erin A. Miller Nikolai Petrovsky David E. Wentworth Victor Bronshtein Mark Papania Stephen M. Tompkins
author_sort	Jasmina M. Luczo
title	Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
title_short	Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
title_full	Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
title_fullStr	Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
title_full_unstemmed	Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
title_sort	intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b30473452fc54337aa97734a70ab5f16
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Intranasal powder live attenuated influenza vaccine is thermostable, immunogenic, and protective against homologous challenge in ferrets

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