Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2

Abstract Semi-dwarf traits have been widely introgressed into cereal crops to improve lodging resistance. In sorghum (Sorghum bicolor L. Moench), four major unlinked dwarfing genes, Dw1-Dw4, have been introduced to reduce plant height, and among them, Dw3 and Dw1 have been cloned. Dw3 encodes a gene...

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Autores principales:	Ko Hirano, Mayuko Kawamura, Satoko Araki-Nakamura, Haruka Fujimoto, Kozue Ohmae-Shinohara, Miki Yamaguchi, Akihiro Fujii, Hiroaki Sasaki, Shigemitsu Kasuga, Takashi Sazuka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b304bdc3fd194680b509e9e790e7ad4e
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spelling	oai:doaj.org-article:b304bdc3fd194680b509e9e790e7ad4e2021-12-02T16:06:25ZSorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 210.1038/s41598-017-00096-w2045-2322https://doaj.org/article/b304bdc3fd194680b509e9e790e7ad4e2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00096-whttps://doaj.org/toc/2045-2322Abstract Semi-dwarf traits have been widely introgressed into cereal crops to improve lodging resistance. In sorghum (Sorghum bicolor L. Moench), four major unlinked dwarfing genes, Dw1-Dw4, have been introduced to reduce plant height, and among them, Dw3 and Dw1 have been cloned. Dw3 encodes a gene involved in auxin transport, whereas, Dw1 was recently isolated and identified as a gene encoding a protein of unknown function. In this study, we show that DW1 is a novel component of brassinosteroid (BR) signaling. Sorghum possessing the mutated allele of Dw1 (dw1), showed similar phenotypes to rice BR-deficient mutants, such as reduced lamina joint bending, attenuated skotomorphogenesis, and insensitivity against feedback regulation of BR-related genes. Furthermore, DW1 interacted with a negative regulator of BR signaling, BRASSINOSTEROID INSENSITIVE 2 (BIN2), and inhibited its nuclear localization, indicating that DW1 positively regulates BR signaling by inhibiting the function of BIN2. In contrast to rice and wheat breeding which used gibberellin (GA) deficiency to reduce plant height, sorghum breeding modified auxin and BR signaling. This difference may result from GA deficiency in rice and wheat does not cause deleterious side effects on plant morphology, whereas in sorghum it leads to abnormal culm bending.Ko HiranoMayuko KawamuraSatoko Araki-NakamuraHaruka FujimotoKozue Ohmae-ShinoharaMiki YamaguchiAkihiro FujiiHiroaki SasakiShigemitsu KasugaTakashi SazukaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ko Hirano Mayuko Kawamura Satoko Araki-Nakamura Haruka Fujimoto Kozue Ohmae-Shinohara Miki Yamaguchi Akihiro Fujii Hiroaki Sasaki Shigemitsu Kasuga Takashi Sazuka Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2
description	Abstract Semi-dwarf traits have been widely introgressed into cereal crops to improve lodging resistance. In sorghum (Sorghum bicolor L. Moench), four major unlinked dwarfing genes, Dw1-Dw4, have been introduced to reduce plant height, and among them, Dw3 and Dw1 have been cloned. Dw3 encodes a gene involved in auxin transport, whereas, Dw1 was recently isolated and identified as a gene encoding a protein of unknown function. In this study, we show that DW1 is a novel component of brassinosteroid (BR) signaling. Sorghum possessing the mutated allele of Dw1 (dw1), showed similar phenotypes to rice BR-deficient mutants, such as reduced lamina joint bending, attenuated skotomorphogenesis, and insensitivity against feedback regulation of BR-related genes. Furthermore, DW1 interacted with a negative regulator of BR signaling, BRASSINOSTEROID INSENSITIVE 2 (BIN2), and inhibited its nuclear localization, indicating that DW1 positively regulates BR signaling by inhibiting the function of BIN2. In contrast to rice and wheat breeding which used gibberellin (GA) deficiency to reduce plant height, sorghum breeding modified auxin and BR signaling. This difference may result from GA deficiency in rice and wheat does not cause deleterious side effects on plant morphology, whereas in sorghum it leads to abnormal culm bending.
format	article
author	Ko Hirano Mayuko Kawamura Satoko Araki-Nakamura Haruka Fujimoto Kozue Ohmae-Shinohara Miki Yamaguchi Akihiro Fujii Hiroaki Sasaki Shigemitsu Kasuga Takashi Sazuka
author_facet	Ko Hirano Mayuko Kawamura Satoko Araki-Nakamura Haruka Fujimoto Kozue Ohmae-Shinohara Miki Yamaguchi Akihiro Fujii Hiroaki Sasaki Shigemitsu Kasuga Takashi Sazuka
author_sort	Ko Hirano
title	Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2
title_short	Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2
title_full	Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2
title_fullStr	Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2
title_full_unstemmed	Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2
title_sort	sorghum dw1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of brassinosteroid insensitive 2
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b304bdc3fd194680b509e9e790e7ad4e
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Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2

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