Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters

With the exception of inactivated vaccines, all SARS-CoV-2 vaccines currently used for clinical application focus on the spike envelope glycoprotein as a virus-specific antigen. Compared to other SARS-CoV-2 genes, mutations in the spike protein gene are more rapidly selected and spread within the po...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jakob Trimpert, Susanne Herwig, Julia Stein, Daria Vladimirova, Julia M. Adler, Azza Abdelgawad, Theresa C. Firsching, Tizia Thoma, Jalid Sehouli, Klaus Osterrieder, Achim D. Gruber, Birgit Sawitzki, Leif Erik Sander, Günter Cichon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 vaccine genes dwarf hamster animal model adenoviral vectors Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b30d56503a0141bb824f2651c751f2d7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b30d56503a0141bb824f2651c751f2d7
record_format	dspace
spelling	oai:doaj.org-article:b30d56503a0141bb824f2651c751f2d72021-11-25T19:14:13ZDeciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters10.3390/v131122901999-4915https://doaj.org/article/b30d56503a0141bb824f2651c751f2d72021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2290https://doaj.org/toc/1999-4915With the exception of inactivated vaccines, all SARS-CoV-2 vaccines currently used for clinical application focus on the spike envelope glycoprotein as a virus-specific antigen. Compared to other SARS-CoV-2 genes, mutations in the spike protein gene are more rapidly selected and spread within the population, which carries the risk of impairing the efficacy of spike-based vaccines. It is unclear to what extent the loss of neutralizing antibody epitopes can be compensated by cellular immune responses, and whether the use of other SARS-CoV-2 antigens might cause a more diverse immune response and better long-term protection, particularly in light of the continued evolution towards new SARS-CoV-2 variants. To address this question, we explored immunogenicity and protective effects of adenoviral vectors encoding either the full-length spike protein (S), the nucleocapsid protein (N), the receptor binding domain (RBD) or a hybrid construct of RBD and the membrane protein (M) in a highly susceptible COVID-19 hamster model. All adenoviral vaccines provided life-saving protection against SARS-CoV-2-infection. The most efficient protection was achieved after exposure to full-length spike. However, the nucleocapsid protein, which triggered a robust T-cell response but did not facilitate the formation of neutralizing antibodies, controlled early virus replication efficiently and prevented severe pneumonia. Although the full-length spike protein is an excellent target for vaccines, it does not appear to be the only option for future vaccine design.Jakob TrimpertSusanne HerwigJulia SteinDaria VladimirovaJulia M. AdlerAzza AbdelgawadTheresa C. FirschingTizia ThomaJalid SehouliKlaus OsterriederAchim D. GruberBirgit SawitzkiLeif Erik SanderGünter CichonMDPI AGarticleSARS-CoV-2vaccine genesdwarf hamsteranimal modeladenoviral vectorsMicrobiologyQR1-502ENViruses, Vol 13, Iss 2290, p 2290 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 vaccine genes dwarf hamster animal model adenoviral vectors Microbiology QR1-502
spellingShingle	SARS-CoV-2 vaccine genes dwarf hamster animal model adenoviral vectors Microbiology QR1-502 Jakob Trimpert Susanne Herwig Julia Stein Daria Vladimirova Julia M. Adler Azza Abdelgawad Theresa C. Firsching Tizia Thoma Jalid Sehouli Klaus Osterrieder Achim D. Gruber Birgit Sawitzki Leif Erik Sander Günter Cichon Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
description	With the exception of inactivated vaccines, all SARS-CoV-2 vaccines currently used for clinical application focus on the spike envelope glycoprotein as a virus-specific antigen. Compared to other SARS-CoV-2 genes, mutations in the spike protein gene are more rapidly selected and spread within the population, which carries the risk of impairing the efficacy of spike-based vaccines. It is unclear to what extent the loss of neutralizing antibody epitopes can be compensated by cellular immune responses, and whether the use of other SARS-CoV-2 antigens might cause a more diverse immune response and better long-term protection, particularly in light of the continued evolution towards new SARS-CoV-2 variants. To address this question, we explored immunogenicity and protective effects of adenoviral vectors encoding either the full-length spike protein (S), the nucleocapsid protein (N), the receptor binding domain (RBD) or a hybrid construct of RBD and the membrane protein (M) in a highly susceptible COVID-19 hamster model. All adenoviral vaccines provided life-saving protection against SARS-CoV-2-infection. The most efficient protection was achieved after exposure to full-length spike. However, the nucleocapsid protein, which triggered a robust T-cell response but did not facilitate the formation of neutralizing antibodies, controlled early virus replication efficiently and prevented severe pneumonia. Although the full-length spike protein is an excellent target for vaccines, it does not appear to be the only option for future vaccine design.
format	article
author	Jakob Trimpert Susanne Herwig Julia Stein Daria Vladimirova Julia M. Adler Azza Abdelgawad Theresa C. Firsching Tizia Thoma Jalid Sehouli Klaus Osterrieder Achim D. Gruber Birgit Sawitzki Leif Erik Sander Günter Cichon
author_facet	Jakob Trimpert Susanne Herwig Julia Stein Daria Vladimirova Julia M. Adler Azza Abdelgawad Theresa C. Firsching Tizia Thoma Jalid Sehouli Klaus Osterrieder Achim D. Gruber Birgit Sawitzki Leif Erik Sander Günter Cichon
author_sort	Jakob Trimpert
title	Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
title_short	Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
title_full	Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
title_fullStr	Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
title_full_unstemmed	Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
title_sort	deciphering the role of humoral and cellular immune responses in different covid-19 vaccines—a comparison of vaccine candidate genes in roborovski dwarf hamsters
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b30d56503a0141bb824f2651c751f2d7
work_keys_str_mv	AT jakobtrimpert decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT susanneherwig decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT juliastein decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT dariavladimirova decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT juliamadler decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT azzaabdelgawad decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT theresacfirsching decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT tiziathoma decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT jalidsehouli decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT klausosterrieder decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT achimdgruber decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT birgitsawitzki decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT leiferiksander decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters AT guntercichon decipheringtheroleofhumoralandcellularimmuneresponsesindifferentcovid19vaccinesacomparisonofvaccinecandidategenesinroborovskidwarfhamsters
_version_	1718410176845840384

Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters

Ejemplares similares