A scaffold-free approach to cartilage tissue generation using human embryonic stem cells

Abstract Articular cartilage functions as a shock absorber and facilitates the free movement of joints. Currently, there are no therapeutic drugs that promote the healing of damaged articular cartilage. Limitations associated with the two clinically relevant cell populations, human articular chondro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lauren A. Griffith, Katherine M. Arnold, Bram G. Sengers, Rahul S. Tare, Franchesca D. Houghton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b31043f49b7b4effa1cec79d6a35537d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b31043f49b7b4effa1cec79d6a35537d
record_format	dspace
spelling	oai:doaj.org-article:b31043f49b7b4effa1cec79d6a35537d2021-12-02T18:51:15ZA scaffold-free approach to cartilage tissue generation using human embryonic stem cells10.1038/s41598-021-97934-92045-2322https://doaj.org/article/b31043f49b7b4effa1cec79d6a35537d2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97934-9https://doaj.org/toc/2045-2322Abstract Articular cartilage functions as a shock absorber and facilitates the free movement of joints. Currently, there are no therapeutic drugs that promote the healing of damaged articular cartilage. Limitations associated with the two clinically relevant cell populations, human articular chondrocytes and mesenchymal stem cells, necessitate finding an alternative cell source for cartilage repair. Human embryonic stem cells (hESCs) provide a readily accessible population of self-renewing, pluripotent cells with perceived immunoprivileged properties for cartilage generation. We have developed a robust method to generate 3D, scaffold-free, hyaline cartilage tissue constructs from hESCs that are composed of numerous chondrocytes in lacunae, embedded in an extracellular matrix containing Type II collagen, sulphated glycosaminoglycans and Aggrecan. The elastic (Young’s) modulus of the hESC-derived cartilage tissue constructs (0.91 ± 0.08 MPa) was comparable to full-thickness human articular cartilage (0.87 ± 0.09 MPa). Moreover, we have successfully scaled up the size of the scaffold-free, 3D hESC-derived cartilage tissue constructs to between 4.5 mm and 6 mm, thus enhancing their suitability for clinical application.Lauren A. GriffithKatherine M. ArnoldBram G. SengersRahul S. TareFranchesca D. HoughtonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lauren A. Griffith Katherine M. Arnold Bram G. Sengers Rahul S. Tare Franchesca D. Houghton A scaffold-free approach to cartilage tissue generation using human embryonic stem cells
description	Abstract Articular cartilage functions as a shock absorber and facilitates the free movement of joints. Currently, there are no therapeutic drugs that promote the healing of damaged articular cartilage. Limitations associated with the two clinically relevant cell populations, human articular chondrocytes and mesenchymal stem cells, necessitate finding an alternative cell source for cartilage repair. Human embryonic stem cells (hESCs) provide a readily accessible population of self-renewing, pluripotent cells with perceived immunoprivileged properties for cartilage generation. We have developed a robust method to generate 3D, scaffold-free, hyaline cartilage tissue constructs from hESCs that are composed of numerous chondrocytes in lacunae, embedded in an extracellular matrix containing Type II collagen, sulphated glycosaminoglycans and Aggrecan. The elastic (Young’s) modulus of the hESC-derived cartilage tissue constructs (0.91 ± 0.08 MPa) was comparable to full-thickness human articular cartilage (0.87 ± 0.09 MPa). Moreover, we have successfully scaled up the size of the scaffold-free, 3D hESC-derived cartilage tissue constructs to between 4.5 mm and 6 mm, thus enhancing their suitability for clinical application.
format	article
author	Lauren A. Griffith Katherine M. Arnold Bram G. Sengers Rahul S. Tare Franchesca D. Houghton
author_facet	Lauren A. Griffith Katherine M. Arnold Bram G. Sengers Rahul S. Tare Franchesca D. Houghton
author_sort	Lauren A. Griffith
title	A scaffold-free approach to cartilage tissue generation using human embryonic stem cells
title_short	A scaffold-free approach to cartilage tissue generation using human embryonic stem cells
title_full	A scaffold-free approach to cartilage tissue generation using human embryonic stem cells
title_fullStr	A scaffold-free approach to cartilage tissue generation using human embryonic stem cells
title_full_unstemmed	A scaffold-free approach to cartilage tissue generation using human embryonic stem cells
title_sort	scaffold-free approach to cartilage tissue generation using human embryonic stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b31043f49b7b4effa1cec79d6a35537d
work_keys_str_mv	AT laurenagriffith ascaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT katherinemarnold ascaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT bramgsengers ascaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT rahulstare ascaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT franchescadhoughton ascaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT laurenagriffith scaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT katherinemarnold scaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT bramgsengers scaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT rahulstare scaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells AT franchescadhoughton scaffoldfreeapproachtocartilagetissuegenerationusinghumanembryonicstemcells
_version_	1718377467286126592

A scaffold-free approach to cartilage tissue generation using human embryonic stem cells

Ejemplares similares