The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

Abstract In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influe...

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Autores principales:	Omar Haque, Casie A. Pendexter, Benjamin T. Wilks, Ehab O. A. Hafiz, James F. Markmann, Korkut Uygun, Heidi Yeh, Shannon N. Tessier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3138742262e448b8923a2d7e9392381
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spelling	oai:doaj.org-article:b3138742262e448b8923a2d7e93923812021-12-05T12:14:32ZThe effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion10.1038/s41598-021-02417-62045-2322https://doaj.org/article/b3138742262e448b8923a2d7e93923812021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02417-6https://doaj.org/toc/2045-2322Abstract In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24 h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 h of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush.Omar HaqueCasie A. PendexterBenjamin T. WilksEhab O. A. HafizJames F. MarkmannKorkut UygunHeidi YehShannon N. TessierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Omar Haque Casie A. Pendexter Benjamin T. Wilks Ehab O. A. Hafiz James F. Markmann Korkut Uygun Heidi Yeh Shannon N. Tessier The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
description	Abstract In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24 h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 h of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush.
format	article
author	Omar Haque Casie A. Pendexter Benjamin T. Wilks Ehab O. A. Hafiz James F. Markmann Korkut Uygun Heidi Yeh Shannon N. Tessier
author_facet	Omar Haque Casie A. Pendexter Benjamin T. Wilks Ehab O. A. Hafiz James F. Markmann Korkut Uygun Heidi Yeh Shannon N. Tessier
author_sort	Omar Haque
title	The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
title_short	The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
title_full	The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
title_fullStr	The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
title_full_unstemmed	The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
title_sort	effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b3138742262e448b8923a2d7e9392381
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The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion

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