Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.

<h4>Background</h4>Scabies outbreaks are common in nursing homes in the Netherlands. In October 2018, a local public health service (PHS) in The Hague was notified of a new scabies outbreak in a nursing home in that region. The PHS initiated an outbreak investigation. Cases were defined...

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Autores principales:	Raïssa Tjon-Kon-Fat, Laurène Peckeu, Susan Hahné, Eric de Coster, Wendy Tas, Bas Wintermans, Anneke Bergmans, Mariska Petrignani, Ewout Fanoy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/b31ac3a1b7c94f9e88f0559b88fc0d45
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spelling	oai:doaj.org-article:b31ac3a1b7c94f9e88f0559b88fc0d452021-11-25T06:33:08ZShort report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.1935-27271935-273510.1371/journal.pntd.0009485https://doaj.org/article/b31ac3a1b7c94f9e88f0559b88fc0d452021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009485https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Scabies outbreaks are common in nursing homes in the Netherlands. In October 2018, a local public health service (PHS) in The Hague was notified of a new scabies outbreak in a nursing home in that region. The PHS initiated an outbreak investigation. Cases were defined as: possible (reported symptoms), probable (scabies-like lesions) and confirmed (PCR or microscopy in skin flakes). Head-to-toe examinations were performed of all residents and those staff members who reported symptoms suggestive of scabies. Skin scrapings of lesions were tested either with microscopy or by PCR. Experimentally for case finding, skin flakes from bed linens of residents who reported symptoms of itchiness but did not have primary lesions were sent for PCR testing.<h4>Principal findings</h4>All residents (41) and 37/44 staff were included in this outbreak investigation. We identified 30 possible, four probable and six confirmed cases. The overall attack rate for probable/confirmed cases was 10/78 (13%). Of the six confirmed cases, two were confirmed by PCR, three by microscopy, and one showed positive findings with both techniques. Two out of the three bed-linen specimens were PCR-positive.<h4>Conclusions</h4>In this outbreak of scabies in a nursing home, PCR was used on skin flakes from bed linens, which led to the detection of two additional cases. This illustrates the potential of PCR during the investigation of scabies outbreaks.Raïssa Tjon-Kon-FatLaurène PeckeuSusan HahnéEric de CosterWendy TasBas WintermansAnneke BergmansMariska PetrignaniEwout FanoyPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009485 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Raïssa Tjon-Kon-Fat Laurène Peckeu Susan Hahné Eric de Coster Wendy Tas Bas Wintermans Anneke Bergmans Mariska Petrignani Ewout Fanoy Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.
description	<h4>Background</h4>Scabies outbreaks are common in nursing homes in the Netherlands. In October 2018, a local public health service (PHS) in The Hague was notified of a new scabies outbreak in a nursing home in that region. The PHS initiated an outbreak investigation. Cases were defined as: possible (reported symptoms), probable (scabies-like lesions) and confirmed (PCR or microscopy in skin flakes). Head-to-toe examinations were performed of all residents and those staff members who reported symptoms suggestive of scabies. Skin scrapings of lesions were tested either with microscopy or by PCR. Experimentally for case finding, skin flakes from bed linens of residents who reported symptoms of itchiness but did not have primary lesions were sent for PCR testing.<h4>Principal findings</h4>All residents (41) and 37/44 staff were included in this outbreak investigation. We identified 30 possible, four probable and six confirmed cases. The overall attack rate for probable/confirmed cases was 10/78 (13%). Of the six confirmed cases, two were confirmed by PCR, three by microscopy, and one showed positive findings with both techniques. Two out of the three bed-linen specimens were PCR-positive.<h4>Conclusions</h4>In this outbreak of scabies in a nursing home, PCR was used on skin flakes from bed linens, which led to the detection of two additional cases. This illustrates the potential of PCR during the investigation of scabies outbreaks.
format	article
author	Raïssa Tjon-Kon-Fat Laurène Peckeu Susan Hahné Eric de Coster Wendy Tas Bas Wintermans Anneke Bergmans Mariska Petrignani Ewout Fanoy
author_facet	Raïssa Tjon-Kon-Fat Laurène Peckeu Susan Hahné Eric de Coster Wendy Tas Bas Wintermans Anneke Bergmans Mariska Petrignani Ewout Fanoy
author_sort	Raïssa Tjon-Kon-Fat
title	Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.
title_short	Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.
title_full	Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.
title_fullStr	Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.
title_full_unstemmed	Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.
title_sort	short report: the potential of pcr on skin flakes from bed linens for diagnosis of scabies in an outbreak.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b31ac3a1b7c94f9e88f0559b88fc0d45
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Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.

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