Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes.
Rhodococcus equi is one of the most widespread causes of disease in foals aged from 1 to 6 months. R. equi possesses antioxidant defense mechanisms to protect it from reactive oxygen metabolites such as hydrogen peroxide (H(2)O(2)) generated during the respiratory burst of phagocytic cells. These de...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b31e12297d6a453d8010da434d4c04dd |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b31e12297d6a453d8010da434d4c04dd |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b31e12297d6a453d8010da434d4c04dd2021-11-18T07:09:35ZRhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes.1932-620310.1371/journal.pone.0042396https://doaj.org/article/b31e12297d6a453d8010da434d4c04dd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22879963/?tool=EBIhttps://doaj.org/toc/1932-6203Rhodococcus equi is one of the most widespread causes of disease in foals aged from 1 to 6 months. R. equi possesses antioxidant defense mechanisms to protect it from reactive oxygen metabolites such as hydrogen peroxide (H(2)O(2)) generated during the respiratory burst of phagocytic cells. These defense mechanisms include enzymes such as catalase, which detoxify hydrogen peroxide. Recently, an analysis of the R. equi 103 genome sequence revealed the presence of four potential catalase genes. We first constructed ΔkatA-, ΔkatB-, ΔkatC-and ΔkatD-deficient mutants to study the ability of R. equi to survive exposure to H(2)O(2)in vitro and within mouse peritoneal macrophages. Results showed that ΔkatA and, to a lesser extent ΔkatC, were affected by 80 mM H(2)O(2). Moreover, katA deletion seems to significantly affect the ability of R. equi to survive within murine macrophages. We finally investigated the expression of the four catalases in response to H(2)O(2) assays with a real time PCR technique. Results showed that katA is overexpressed 367.9 times (± 122.6) in response to exposure to 50 mM of H(2)O(2) added in the stationary phase, and 3.11 times (± 0.59) when treatment was administered in the exponential phase. In untreated bacteria, katB, katC and katD were overexpressed from 4.3 to 17.5 times in the stationary compared to the exponential phase. Taken together, our results show that KatA is the major catalase involved in the extreme H(2)O(2) resistance capability of R. equi.Pauline BidaudLaurent HébertCorinne BarbeyAnne-Cécile AppourchauxRiccardo TorelliMaurizio SanguinettiClaire LaugierSandrine PetryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42396 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Pauline Bidaud Laurent Hébert Corinne Barbey Anne-Cécile Appourchaux Riccardo Torelli Maurizio Sanguinetti Claire Laugier Sandrine Petry Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes. |
| description |
Rhodococcus equi is one of the most widespread causes of disease in foals aged from 1 to 6 months. R. equi possesses antioxidant defense mechanisms to protect it from reactive oxygen metabolites such as hydrogen peroxide (H(2)O(2)) generated during the respiratory burst of phagocytic cells. These defense mechanisms include enzymes such as catalase, which detoxify hydrogen peroxide. Recently, an analysis of the R. equi 103 genome sequence revealed the presence of four potential catalase genes. We first constructed ΔkatA-, ΔkatB-, ΔkatC-and ΔkatD-deficient mutants to study the ability of R. equi to survive exposure to H(2)O(2)in vitro and within mouse peritoneal macrophages. Results showed that ΔkatA and, to a lesser extent ΔkatC, were affected by 80 mM H(2)O(2). Moreover, katA deletion seems to significantly affect the ability of R. equi to survive within murine macrophages. We finally investigated the expression of the four catalases in response to H(2)O(2) assays with a real time PCR technique. Results showed that katA is overexpressed 367.9 times (± 122.6) in response to exposure to 50 mM of H(2)O(2) added in the stationary phase, and 3.11 times (± 0.59) when treatment was administered in the exponential phase. In untreated bacteria, katB, katC and katD were overexpressed from 4.3 to 17.5 times in the stationary compared to the exponential phase. Taken together, our results show that KatA is the major catalase involved in the extreme H(2)O(2) resistance capability of R. equi. |
| format |
article |
| author |
Pauline Bidaud Laurent Hébert Corinne Barbey Anne-Cécile Appourchaux Riccardo Torelli Maurizio Sanguinetti Claire Laugier Sandrine Petry |
| author_facet |
Pauline Bidaud Laurent Hébert Corinne Barbey Anne-Cécile Appourchaux Riccardo Torelli Maurizio Sanguinetti Claire Laugier Sandrine Petry |
| author_sort |
Pauline Bidaud |
| title |
Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes. |
| title_short |
Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes. |
| title_full |
Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes. |
| title_fullStr |
Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes. |
| title_full_unstemmed |
Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes. |
| title_sort |
rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/b31e12297d6a453d8010da434d4c04dd |
| work_keys_str_mv |
AT paulinebidaud rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes AT laurenthebert rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes AT corinnebarbey rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes AT annececileappourchaux rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes AT riccardotorelli rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes AT mauriziosanguinetti rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes AT clairelaugier rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes AT sandrinepetry rhodococcusequisextremeresistancetohydrogenperoxideismainlyconferredbyoneofitsfourcatalasegenes |
| _version_ |
1718423875555950592 |